FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11904546
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7584856.371389551520906No Hit
TCAGTGCACTACAGAACTTTGT2678522.2499976059565814No Hit
AAACCGTTACCATTACTGAGC2067631.7368406993429235No Hit
TTCAAGTAATCCAGGATAGGCT1857631.5604375000945017No Hit
TATTGCACTTGTCCCGGCCTGT1638171.376087756727556No Hit
TGAGGGGCAGAGAGCGAGACTTT1467191.2324619519299602No Hit
CGCGACCTCAGATCAGA1340781.1262756261347555No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1282941.0776891449703332No Hit
TCCTGTACTGAGCTGCCCCGA958170.8048773972564767No Hit
TAGCTTATCAGACTGATGTTGAC945760.7944528081961295No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT830770.6978594563791009No Hit
TCCTGTACTGAGCTGCCCCGAG799190.6713317752730764No Hit
TCAGTGCACTACAGAACTTTGC756970.6358663320717984No Hit
AAGCTGCCAGTTGAAGAACTGT739960.6215776729326763No Hit
CGCGACCTCAGATCAGACGT688290.5781740857652194No Hit
CCCATAAAGTAGAAAGCACT673960.5661366674546009No Hit
TGAGGTAGTAGGTTGTGTGGTT661180.5554012727574827No Hit
TGAGGTAGTAGGTTGTGTGGT615170.5167521718173881No Hit
TTCAAGTAATCCAGGATAGGCC592000.49728901883364557No Hit
TGAGGTAGTAGGTTGTATAGT564430.4741297988180314No Hit
TATTGCACTTGTCCCGGCCTGC553750.46515843611339736No Hit
TTCACAGTGGCTAAGTTCCG545300.4580603073817347No Hit
TGGAGTGTGACAATGGTGTTT545050.45785030357310563No Hit
GGCTGGTCCGATGGTAGT512460.4304742070802196No Hit
TGAGGTAGTAGTTTGTACAGT508180.4268789418764899No Hit
TAGCTTATCAGACTGATGTTGA501080.42091483371142424No Hit
TGAGGGGCAGAGAGCGAGACT488380.4102466402330673No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT483480.40613056558393745No Hit
TGAGGTAGTAGTTTGTGCTGTT473840.39803281872320034No Hit
TGAGGTAGTAGGTTGTATAGTT467500.39270712213636705No Hit
TGAGGTAGTAGATTGTATAGTT460840.38711262067448854No Hit
CGCGACCTCAGATC442390.37161433959766293No Hit
TGTAAACATCCCCGACTGGAAGCT439580.36925389678867215No Hit
TCCTGTACTGAGCTGCCCCGAGT439010.3687750881049979No Hit
AAACCGTTACCATTACTGAG434450.36494461863560357No Hit
TGTAAACATCCTTGACTGGAAGCT382240.3210874232415079No Hit
TGAGGGGCAGAGAGCGAGACTTC370990.3116372518531996No Hit
GGCTGGTCCGATGGTAGTGGGTT357750.3005154501482039No Hit
GTTTCCGTAGTGTAGTGGTTATC355660.29875981830806486No Hit
TCCTGTACTGAGCTGCCCCGAGA350030.2940305325377381No Hit
TGGCTCAGTTCAGCAGGAAC349910.29392973070959616No Hit
GGCTGGTCCGATGGTAGTGGGT325110.2730973528935921No Hit
TAGCTTATCAGACTGATGTTGAT319790.2686284718459654No Hit
TGAGGTAGTAGATTGTATAGT316530.26589002218144225No Hit
TAGCTTATCAGACTGATGTTG309770.26021151919611213No Hit
TGAGGGGCAGAGAGCGAGACTT291130.24455363522472842No Hit
CGCGACCTCAGATCAGAC285480.2398075491497114No Hit
TCCTGTACTGAGCTGCCCCG284190.23872392949718535No Hit
AGCTCGGTCTGAGGCCCCTCAGT281180.2361954836412913No Hit
TGAGAACTGAATTCCATGGGTT267840.22498968041284395No Hit
TGAGATGAAGCACTGTAGCT266580.22393126121735343No Hit
GGCTGGTCCGATGGTAGTG260970.21921877575171705No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC259800.218235957927333No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT238560.20039403434620692No Hit
TTCAAGTAATTCAGGATAGGT221240.1858449704843847No Hit
AAGCTGCCAGTTGAAGAACTGC218320.18339212599959712No Hit
TGAGGTAGTAGGTTGTGTGGTC215050.18064527618272885No Hit
CGCGACCTCAGATCAGACGC201910.16960747600118475No Hit
CCCATAAAGTAGAAAGCACC199000.16716303166874233No Hit
TGTAAACATCCCCGACTGGAAG194800.16363496768377392No Hit
AGAAGCCGGCGGGAGC194170.16310575808602865No Hit
TGAGGTAGTAGGTTGTGTGGC183960.1545292025416173No Hit
AAACCGTTACCATTACTGAGTT180620.15172355165833287No Hit
TGTAAACATCCCCGACTGGAAGC180150.1513287444981102No Hit
TGAGGTAGTAGTTTGTGCTGT175860.1477250791420353No Hit
TCGTACCGTGAGTAATAATGCG174320.14643145568088023No Hit
TACAGTACTGTGATAACTGAAG173930.14610384973941887No Hit
AAACCGTTACCATTACTG173480.14572584288388654No Hit
TGAGGTAGTAGGTTGTGTGG165990.1394341287773595No Hit
ACCAATATTACTGTGCTGCTT165820.13929132618749174No Hit
TTCAAGTAATCCAGGATAGGCA162640.13662007774172993No Hit
TCCCTGGTGGTCTAGTGGTTAGGATT162010.13609086814398466No Hit
GCATTGGTGGTTCAGTGGTAGAATT161060.13529285367119417No Hit
AAGCTGCCAGTTGAAGAACT158850.13343642000291317No Hit
TATTGCACTTGTCCCGGCCTG157990.13271400690122917No Hit
TGTCTGAGCGTCGCT157050.13192439258078384No Hit
TGAGGTAGTAGGTTGTATAGC154940.13015196043595448No Hit
TGAGATGAAGCACTGTAGCTC154310.1296227508382092No Hit
TGAGGGGCAGAGAGCGAGACC151430.12720350696280228No Hit
TGGAGTGTGACAATGGTGTTC151400.12717830650576678No Hit
TGTAAACATCCCCGACTGGAAGCC148120.12442305653655335No Hit
TCACAGTGAACCGGTCTCTTT148030.12434745516544689No Hit
CAACGGAATCCCAAAAGCAGCT146850.12335623718871766No Hit
AAACCGTTACCATTACTGAGA145650.12234821890729809No Hit
GGCTGGTCCGATGGTAGC144890.12170980732906572No Hit
TGAGGTAGTAGTTTGTGCTGTC144520.12139900169229469No Hit
TACAGTATAGATGATGTACT144460.12134860077822371No Hit
CCTGTCTGAGCGTCGCT143950.1209201930086204No Hit
TGAGGTAGTAGGTTGTATAGTC143270.12034898264914931No Hit
GGCTGGTCCGATGGTAGTGGG143110.12021458021162672No Hit
TGAGGTAGTAGGTTGTAT138710.11651851317975503No Hit
TGAGGTAGTAGTTTGTACAGC138540.11637571058988727No Hit
TGAGGTAGTAGATTGTATAGTC136800.1149140840818289No Hit
AACCCGTAGATCCGATCTTGT136560.11471248042554501No Hit
ACGCCTGTCTGAGCGTCGCT134080.1126292426439446No Hit
GGCTGGTCCGATGGTAGTGGGTTATC131260.11026039968260865No Hit
GTCTGAGCGTCGCT130850.10991599343645696No Hit
TGAGGTAGTAGGTTGTATAG129510.10879037302220514No Hit
TGTAAACATCCCCGACTGGAAGT126940.10663153386949827No Hit
TGTAAACATCCTTGACTGGAAGCC126840.10654753234604662No Hit
ACCAATATTACTGTGCTGCTTT124750.10479190050590757No Hit
TCCTGTACTGAGCTGCCCCGAGC124700.10474989974418175No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG124330.10443909410741074No Hit
AAAAGCTGGGTTGAGAGGGCGA124310.10442229380272042No Hit
AACATTCAACGCTGTCGGTGA122850.10319587156032663No Hit
TTCACAGTGGCTAAGTTCCA119720.10056662387629063No Hit
TGAGGTAGTAGTTTGTACAGTT119290.10020541732544862No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)