FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11230622
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9566078.517845227094279No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2910432.5915127407903142No Hit
TCAGTGCACTACAGAACTTTGT2840262.5290317847043555No Hit
AAACCGTTACCATTACTGAGC1872171.6670225389119144No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1741421.5505997797806748No Hit
TATTGCACTTGTCCCGGCCTGT1638431.458895152913169No Hit
TTCAAGTAATCCAGGATAGGCT1484411.3217522591357806No Hit
TCCTGTACTGAGCTGCCCCGAG1262171.1238647334047926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1146411.0207894095269165No Hit
TCCTGTACTGAGCTGCCCCGA1026770.9142592458369625No Hit
TGAGGGGCAGAGAGCGAGACTTT891890.7941590412356502No Hit
AAGCTGCCAGTTGAAGAACTGT853150.759664068472788No Hit
AAACCGTTACCATTACTGAGG826660.7360767729516673No Hit
TAGCTTATCAGACTGATGTTGAC722850.643641999525939No Hit
TGAGGTAGTAGGTTGTATAGTT649870.5786589558441197No Hit
CGCGACCTCAGATCAGA642760.5723280509307499No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT614700.5473427918774223No Hit
TCAGTGCACTACAGAACTTTGC590720.5259904571625685No Hit
TGAGGTAGTAGGTTGTATAGT578380.5150026418839491No Hit
TGGAGTGTGACAATGGTGTTT556490.4955112904699312No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT546750.4868385740344569No Hit
TGAGGGGCAGAGAGCGAGACT531570.4733219584810174No Hit
CCCATAAAGTAGAAAGCACT521650.4644889659717868No Hit
TGAGGTAGTAGATTGTATAGTT516160.45960054572222264No Hit
TAGCTTATCAGACTGATGTTGA504050.4488175276489583No Hit
TGAGGTAGTAGTTTGTGCTGTT503730.44853259240672505No Hit
AAACCGTTACCATTACTGAG495100.44084824509274734No Hit
CATTGCACTTGTCTCGGTCTGA484660.43155223281488775No Hit
TATTGCACTTGTCCCGGCCTGC418720.37283776446219985No Hit
TCCTGTACTGAGCTGCCCCG410040.3651088960166231No Hit
TGAGGTAGTAGATTGTATAGT402750.358617715029497No Hit
TGAGGTAGTAGTTTGTACAGT402540.35843072627678146No Hit
TTCACAGTGGCTAAGTTCCG401550.3575492078711224No Hit
TGAGGTAGTAGGTTGTGTGGTT399200.355456714685972No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC392730.34969568025706854No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC365500.32544947198828345No Hit
TTCAAGTAATCCAGGATAGGCC363140.3233480745768133No Hit
TGAGGTAGTAGGTTGTGTGGT348330.31016091539720597No Hit
TCGTACCGTGAGTAATAATGCG332170.29577168566442713No Hit
TCCTGTACTGAGCTGCCCCGAGT323270.28784692423981506No Hit
TGGCTCAGTTCAGCAGGAAC323200.28778459465557654No Hit
TCCTGTACTGAGCTGCCCCGAGA313380.27904064440954385No Hit
AAACCGTTACCATTACTGAGTT312860.2785776246409148No Hit
TAGCTTATCAGACTGATGTTG300300.26739391638326No Hit
CGCGACCTCAGATC299570.2667439078619154No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT290230.2584273604792326No Hit
TGAGAACTGAATTCCATGGGTT270100.24050315289749755No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC262280.23354004791542268No Hit
CGCGACCTCAGATCAGACGT259290.2308776842458058No Hit
TCAGTGCACTACAGAACTTTGG255360.22737832330212876No Hit
AAAAGCTGGGTTGAGAGGGCGA248690.22143920434682957No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG246920.21986315628822695No Hit
TGAGATGAAGCACTGTAGCT242560.215980913612799No Hit
TGTAAACATCCTTGACTGGAAGCT228540.203497188312455No Hit
TGTAAACATCCCCGACTGGAAGCT226310.2015115458431421No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC225610.20088825000075688No Hit
TGAGGTAGTAGTTTGTACAGTT224350.19976631748446347No Hit
GCATTGGTGGTTCAGTGGTAGAATT215540.19192169409672946No Hit
TGTAAACATCCCCGACTGGAAG206490.18386336927732053No Hit
AAACCGTTACCATTACTG205360.18285719170318437No Hit
TTCAAGTAATCCAGGATAGGCG202650.18044414637052159No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT190390.16952756490246043No Hit
TGAGGGGCAGAGAGCGAGACTT186970.16648231950109263No Hit
AAGCTGCCAGTTGAAGAACTGC186270.16585902365870742No Hit
TGAGGTAGTAGGTTGTATAG175510.15627807613861458No Hit
CATTGCACTTGTCTCGGTCTG173950.15488901683272752No Hit
TATTGCACTTGTCCCGGCCTGG171850.15301912930557185No Hit
TTCAAGTAATTCAGGATAGGT171130.15237802501054706No Hit
TAGCAGCACGTAAATATTGGCG170410.15173692071552225No Hit
TGAGGTAGTAGGTTGTGTGG169620.15103348683625895No Hit
AAACCGTTACCATTACTGAGA168460.15000059658316342No Hit
CTAGACTGAAGCTCCTTGAGG167130.1488163344826315No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG164800.1467416497501207No Hit
TGAGGTAGTAGTTTGTGCTGT162860.14501422984408166No Hit
TGAGATGAAGCACTGTAGCTC160530.14293954511157086No Hit
TACAGTACTGTGATAACTGAAG153920.1370538515141904No Hit
TGGCTCAGTTCAGCAGGAACAG152750.1360120570347751No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT151630.13501478368695874No Hit
AGCTCGGTCTGAGGCCCCTCAGT146820.13073185082714028No Hit
TCAGTGCATCACAGAACTTTGT143680.12793592376272658No Hit
TTCAAGTAATCCAGGATAGGCA141150.12568315450382No Hit
AAGCTGCCAGTTGAAGAACT138310.12315435422899997No Hit
TGAGGGGCAGAGAGCGAGACTTC137700.12261119642349284No Hit
TAGCTTATCAGACTGATGTTGAT137440.12237968653917833No Hit
TATTGCACTTGTCCCGGCCTG135190.12037623561722582No Hit
TGAGGTAGTAGGTTGTATAGTC134760.1199933538854749No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT132720.11817689171623798No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA130030.11578165483621476No Hit
CAACGGAATCCCAAAAGCAGCTG127150.11321723765611558No Hit
GCGCGGGACATGTGGCGTACGGAAG122170.10878293294886072No Hit
TCCTGTACTGAGCTGCCCCGAA118860.10583563403701059No Hit
AGGAAACTCTGGTG117980.10505206212086918No Hit
CGGACAGGATTGACAGA117930.10500754098927023No Hit
GGCTGGTCCGATGGTAGT117740.10483836068919425No Hit
TGAGGTAGTAGGTTGTATAGC114510.10196229558790243No Hit
TGAGGGGCAGAGAGCGAGACC114040.10154379695087236No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)