FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13532977
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT192185714.20128771370852No Hit
AAACCGTTACCATTACTGAGC3851872.8462843024118047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2437011.8007937204060867No Hit
TCCTGTACTGAGCTGCCCCGAG2324191.7174269933363517No Hit
TATTGCACTTGTCCCGGCCTGT2289151.6915346859748597No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2195371.6222372948686754No Hit
TCCTGTACTGAGCTGCCCCGA1892381.398347163377282No Hit
TCAGTGCACTACAGAACTTTGT1888121.3951992972425802No Hit
TGAGGTAGTAGGTTGTATAGT1767661.3061871013303281No Hit
TGGAGTGTGACAATGGTGTTT1608501.1885780933493053No Hit
AAACCGTTACCATTACTGAGG1549971.1453281861042104No Hit
TTCAAGTAATCCAGGATAGGCT1434121.0597224838259904No Hit
TGAGGTAGTAGGTTGTGTGGT1331240.9837007777372265No Hit
CGCGACCTCAGATCAGA1302580.9625228802206639No Hit
TGAGGTAGTAGGTTGTGTGGTT1137140.840273355965949No Hit
TGAGGTAGTAGGTTGTATAGTT1129400.8345539935521947No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1085180.8018782563511341No Hit
TGAGGTAGTAGATTGTATAGT966930.7144991083632227No Hit
TGAGGGGCAGAGAGCGAGACTTT884340.6534704078784734No Hit
TGAGGGGCAGAGAGCGAGACT842020.6221986485309182No Hit
TCCTGTACTGAGCTGCCCCGAGT762500.5634384806831491No Hit
TCCTGTACTGAGCTGCCCCGAGA753760.5569801825570234No Hit
TGAGGTAGTAGTTTGTGCTGTT746330.5514898902141044No Hit
TCCTGTACTGAGCTGCCCCG742620.5487484387212067No Hit
TGAGGTAGTAGATTGTATAGTT705840.5215703832201888No Hit
AAGCTGCCAGTTGAAGAACTGT687430.5079665767554322No Hit
AAACCGTTACCATTACTGAG647390.47837959083208376No Hit
TAGCTTATCAGACTGATGTTGAC618650.4571425784585313No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT605180.44718911441288933No Hit
TGAGGTAGTAGTTTGTACAGT602940.4455338984171775No Hit
TATTGCACTTGTCCCGGCCTGC582070.4301123100999876No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT554310.4095994547245591No Hit
AAACCGTTACCATTACTGAGA508880.376029605311529No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC505880.373812798174415No Hit
CCCATAAAGTAGAAAGCACT501340.3704580300402491No Hit
TCGTACCGTGAGTAATAATGCG499090.36879542468741355No Hit
AAACCGTTACCATTACTG476110.35181468201712013No Hit
TGAGGTAGTAGGTTGTATAG461790.34123312261596245No Hit
TGAGGTAGTAGGTTGTGTGG454850.33610490877210536No Hit
TCAGTGCACTACAGAACTTTGC411050.30373952457024056No Hit
TAGCTTATCAGACTGATGTTGA392400.289958373534515No Hit
TGTAAACATCCCCGACTGGAAGCT385130.28458631090557535No Hit
CATTGCACTTGTCTCGGTCTGA377470.2789260633488108No Hit
TATTGCACTTGTCCCGGCCTG360350.2662754839530134No Hit
TGAGGTAGTAGGTTGTATAGC359520.26566216731174525No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC354510.26196009939276477No Hit
TTCAAGTAATCCAGGATAGGCC347490.25677277069191795No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT320700.23697668295748966No Hit
TGGAGTGTGACAATGGTGTTC317390.23453080574954055No Hit
TAGCTTATCAGACTGATGTTG316470.23385098489415893No Hit
TTCAAGTAATTCAGGATAGGT309180.2284641435509718No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG308290.22780649076696133No Hit
TCCTGTACTGAGCTGCCCCGAA303920.22457734170389856No Hit
TGAGGTAGTAGGTTGTGTGGC302380.2234393807068467No Hit
TGAGGTAGTAGGTTGTGTGGTC263090.19440659656777662No Hit
TGAGGTAGTAGGTTGTATAGTC256360.18943355922351748No Hit
AAAAGCTGGGTTGAGAGGGCGA248070.18330778216795904No Hit
CATTGCACTTGTCTCGGTCTG238810.17646523747140042No Hit
TGAGGTAGTAGGTTGTAT236420.1746991811188329No Hit
TGAGGGGCAGAGAGCGAGACTT233780.17274839083817256No Hit
TGAGGTAGTAGATTGTATAG230840.1705759198438008No Hit
TATTGCACTTGTCCCGGCCTGG221360.16357080929052048No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA215420.1591815311590347No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT210290.15539079095456973No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG209880.15508782731249746No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT209750.1549917656698892No Hit
TGAGGTAGTAGTTTGTACAGTT209600.1548809253130335No Hit
TGGCTCAGTTCAGCAGGAAC207010.1529670818179917No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT206460.15256066717618746No Hit
CGCGACCTCAGATCAGACGT204190.15088328310910454No Hit
AAACCGTTACCATTACTGAGTT203270.15020346225372289No Hit
AAGCTGCCAGTTGAAGAACT199250.14723294068999007No Hit
TCCTGTACTGAGCTGCCCCA197930.14625754554965992No Hit
TGAGGTAGTAGTTTGTGCTGT197260.1457624586223711No Hit
TGAGGGGCAGAGAGCGAGACC194950.1440555171267933No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC193090.1426810967017826No Hit
CTAGACTGAAGCTCCTTGAGG192530.14226729270285465No Hit
TGAGGTAGTAGATTGTATAGC191230.1413066762767719No Hit
TGTAAACATCCCCGACTGGAAG190520.14078203192098826No Hit
TGAGGTAGTAGATTGTAT189860.1402943343508232No Hit
TTCAAGTAATCCAGGATAGGCA187160.13829920792742054No Hit
TTCAAGTAATCCAGGATAGGCG183830.13583855200522396No Hit
ACCAATATTACTGTGCTGCTT183260.13541735864917231No Hit
CAACGGAATCCCAAAAGCAGCT181250.13393209786730592No Hit
TGTAAACATCCTTGACTGGAAGCT177560.13120542508865565No Hit
TGGAGTGTGACAATGGTGTTTG173970.12855264588124252No Hit
TTCACAGTGGCTAAGTTCCG173590.12827185031054142No Hit
TACAGTACTGTGATAACTGAAG171350.1266166343148296No Hit
AGCTCGGTCTGAGGCCCCTCAGT171180.1264910152437265No Hit
TGAGGTAGTAGTTTGTGCTGTC164650.12166576504194161No Hit
TATTGCACTTGTCCCGGCCTGTA162730.12024700847418865No Hit
TATTGCACTTGTCCCGGCCTGA161790.11955240890455959No Hit
TCAGTGCACTACAGAACTTTGG160110.11831099690777572No Hit
TCCTGTACTGAGCTGCCCCGAGC158690.11726170819620842No Hit
TGAGGGGCAGAGAGCGAGACTTC156910.11594640262818742No Hit
TGTAAACATCCCCGACTGGAAGC156350.11553259862925946No Hit
TGGCTCAGTTCAGCAGGAACAG155410.11483799905963041No Hit
TCCTGTACTGAGCTGCCCCGG155030.1145572034889293No Hit
AAACCGTTACTATTACTGAGT153570.11347835734886713No Hit
AAGCTGCCAGTTGAAGAACTGC152350.1125768557797741No Hit
AACCCGTAGATCCGATCTTGT150400.11113593114064999No Hit
TAGCTTATCAGACTGATGTTGAT148370.10963589164453616No Hit
AGAGGTAGTAGGTTGCATAGT144970.10712351022247359No Hit
CGCGACCTCAGATC142530.10532050708428752No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT139130.10280812566222494No Hit
TGAGGTAGTAGTTTGTGCT138930.10266033851975068No Hit
TGAGGTAGTAGATTGTATAGTC136650.10097556509554402No Hit
AGACCGTTACCATTACTGAGT135970.10047308881113151No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT135380.1000371167408324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)