FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11632727
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT133130511.444479011671124No Hit
AAACCGTTACCATTACTGAGC2498192.147553191955764No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2314321.9894905124138131No Hit
TCCTGTACTGAGCTGCCCCGA2172671.8677219881460299No Hit
TATTGCACTTGTCCCGGCCTGT2112031.8155931966769272No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1954971.68057756362717No Hit
TGAGGTAGTAGGTTGTATAGT1859001.5980775616929719No Hit
TCCTGTACTGAGCTGCCCCGAG1761981.5146749339170429No Hit
TTCAAGTAATCCAGGATAGGCT1688791.4517576145301099No Hit
TGAGGTAGTAGGTTGTGTGGT1431881.2309065621500443No Hit
CGCGACCTCAGATCAGA1382201.1881994651812942No Hit
TGAGGTAGTAGGTTGTATAGTT1337141.1494639219161595No Hit
TGAGGTAGTAGGTTGTGTGGTT1318121.1331134995259495No Hit
TGGAGTGTGACAATGGTGTTT1231541.0586855515478013No Hit
TCAGTGCACTACAGAACTTTGT1201641.0329822061499423No Hit
TGAGGTAGTAGATTGTATAGT1055100.9070100243906695No Hit
TGAGGTAGTAGATTGTATAGTT956870.822567227787603No Hit
TGAGGGGCAGAGAGCGAGACTTT826600.7105814483568642No Hit
TCCTGTACTGAGCTGCCCCGAGA773730.6651320881165698No Hit
TCCTGTACTGAGCTGCCCCGAGT759220.6526586586275084No Hit
TGAGGGGCAGAGAGCGAGACT703230.6045272101717852No Hit
TGAGGTAGTAGTTTGTGCTGTT699580.601389510817197No Hit
TAGCTTATCAGACTGATGTTGAC626030.5381627197130991No Hit
AAGCTGCCAGTTGAAGAACTGT613990.5278126100612522No Hit
TGAGGTAGTAGTTTGTACAGT612670.5266778804316476No Hit
TCCTGTACTGAGCTGCCCCG544270.46787825417032486No Hit
TCGTACCGTGAGTAATAATGCG515210.44289700944585053No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC503320.4326758463428223No Hit
TATTGCACTTGTCCCGGCCTGC492440.4233229233351733No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT422570.3632596208954272No Hit
TTCAAGTAATCCAGGATAGGCC381340.3278165128434631No Hit
CCCATAAAGTAGAAAGCACT378940.3257533680623641No Hit
TGTAAACATCCCCGACTGGAAGCT369150.31733745664279756No Hit
AAACCGTTACCATTACTGAG366070.3146897541737204No Hit
TGAGGTAGTAGGTTGTATAG360120.30957487440391235No Hit
TGAGGTAGTAGGTTGTGTGG359770.30927399912333536No Hit
TGAGGTAGTAGGTTGTATAGC351390.30207018526266455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC334550.28759378604861957No Hit
TAGCTTATCAGACTGATGTTGA324630.27906612095341016No Hit
TTCAAGTAATTCAGGATAGGT314780.27059863091431613No Hit
TGAGGTAGTAGGTTGTGTGGC299400.2573773114421064No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG298990.25702485754200194No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT298160.2563113533052052No Hit
CATTGCACTTGTCTCGGTCTGA292290.25126524502810044No Hit
TGAGGTAGTAGGTTGTGTGGTC287990.24756877729529803No Hit
CGCGACCTCAGATCAGACGT286050.24590106859724292No Hit
AAACCGTTACCATTACTGAGA282430.24278915855241853No Hit
TGAGGTAGTAGGTTGTATAGTC279030.2398663701125282No Hit
TATTGCACTTGTCCCGGCCTG272380.23414973978156625No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT271340.23325571037642337No Hit
AAACCGTTACCATTACTG270980.23294623865925848No Hit
TAGCTTATCAGACTGATGTTG263140.22620663237433491No Hit
TCCTGTACTGAGCTGCCCCGAA257130.2210401739849994No Hit
TGAGGTAGTAGGTTGTAT245500.21104251823325693No Hit
AAAAGCTGGGTTGAGAGGGCGA244490.21017427813787773No Hit
TCAGTGCACTACAGAACTTTGC236880.20363238989447616No Hit
TGGAGTGTGACAATGGTGTTC227490.19556033593842612No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC211840.18210691267834275No Hit
TGAGGGGCAGAGAGCGAGACTT211110.18147937280742513No Hit
TGAGGTAGTAGTTTGTACAGTT209870.18041341467052396No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT205900.1770006293451226No Hit
TGGCTCAGTTCAGCAGGAAC202480.17406064803205645No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT200320.17220381772906732No Hit
TGAGGTAGTAGATTGTATAGC197920.17014067294796825No Hit
TGAGGTAGTAGTTTGTGCTGT197370.16966786893563304No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA195500.16806033529369338No Hit
TGAGGTAGTAGATTGTATAGTC192580.16555017581002288No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT189750.16311738425564357No Hit
TGAGGTAGTAGATTGTAT185200.1592060056081433No Hit
AAGCTGCCAGTTGAAGAACT180100.15482182294830782No Hit
AGCTCGGTCTGAGGCCCCTCAGT177410.1525093815061593No Hit
TGAGGTAGTAGATTGTATAG175250.15065255120317017No Hit
TTCAAGTAATCCAGGATAGGCA174070.14963817168579646No Hit
CAACGGAATCCCAAAAGCAGCT160720.138161928840933No Hit
TCCTGTACTGAGCTGCCCCA156460.13449984685448219No Hit
TAGCTTATCAGACTGATGTTGAT155020.13326195998582274No Hit
TGAGGGGCAGAGAGCGAGACC152780.13133635819013031No Hit
TCCTGTACTGAGCTGCCCCGAGC151190.1299695247726522No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT151020.12982338535065768No Hit
GGCTGGTCCGATGGTAGTGGGTT150200.12911847755044883No Hit
ACCAATATTACTGTGCTGCTT148250.12744217241580585No Hit
CTAGACTGAAGCTCCTTGAGG144380.12411535145628363No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT144320.12406377283675615No Hit
TATTGCACTTGTCCCGGCCTGTA144240.12399500134405285No Hit
AGAGGTAGTAGGTTGCATAGT143890.1236941260634759No Hit
TCGTACCGTGAGTAATAATGCA143690.12352219733171765No Hit
TGTAAACATCCTTGACTGGAAGCT140670.12092607348216802No Hit
TGAGGTAGTAGTTTGTGCTGTC140540.12081431980652516No Hit
TACCCTGTAGAACCGAATTTGT140500.12077993406017351No Hit
TGAGGGGCAGAGAGCGAGACTTC139730.12011800844290424No Hit
TACAGTATAGATGATGTACT133850.11506330372921157No Hit
CATTGCACTTGTCTCGGTCTG133700.11493435718039288No Hit
AGAGGTAGTAGGTTGCATAGTT133670.11490856787062913No Hit
AAACCGTTACCATTACTGAGG131890.11337840215798066No Hit
TGAGAACTGAATTCCATGGGTT129790.11157315047451902No Hit
TGTAAACATCCCCGACTGGAAGC127350.1094756199470683No Hit
TATTGCACTTGTCCCGGCCTGA126060.10836667962722756No Hit
AAGCTGCCAGTTGAAGAACTGC125870.10820334733205722No Hit
CGCGACCTCAGATC125670.10803141860029897No Hit
AACCCGTAGATCCGATCTTGT125050.10749843953184839No Hit
TGGCTCAGTTCAGCAGGAACAG122460.10527196245557899No Hit
TGAGGTAGTAGTTTGTGCT122070.10493670142865039No Hit
TTCACAGTGGCTAAGTTCCG118830.10215145597416667No Hit
TGAGGTAGTAGTTTGTACAGC117550.10105111209091386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)