FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14969926
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT195637113.068675155775653No Hit
AAACCGTTACCATTACTGAGC3476422.322269328519059No Hit
TCCTGTACTGAGCTGCCCCGAG2790531.864090710936046No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2740831.830890814022728No Hit
TGAGGTAGTAGGTTGTATAGT2537881.6953190015768949No Hit
TATTGCACTTGTCCCGGCCTGT2499551.6697143325892192No Hit
TCCTGTACTGAGCTGCCCCGA2442281.6314576304518806No Hit
TCAGTGCACTACAGAACTTTGT2388841.5957593911953873No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2169571.4492857212520622No Hit
TTCAAGTAATCCAGGATAGGCT1885311.2593983430512616No Hit
TGAGGTAGTAGGTTGTGTGGT1782901.1909878512425511No Hit
TGAGGTAGTAGGTTGTATAGTT1682221.123733009769053No Hit
TGAGGTAGTAGGTTGTGTGGTT1611591.076551747817591No Hit
CGCGACCTCAGATCAGA1502411.003618855564149No Hit
TGAGGTAGTAGATTGTATAGT1333370.8906991257004209No Hit
TCCTGTACTGAGCTGCCCCGAGA1121830.7493891419369741No Hit
TCCTGTACTGAGCTGCCCCGAGT1110120.741566791980134No Hit
TGAGGTAGTAGATTGTATAGTT1005700.6718136081634605No Hit
TGAGGTAGTAGTTTGTGCTGTT998380.6669238044329678No Hit
TGAGGGGCAGAGAGCGAGACT917030.6125815184390357No Hit
TGAGGGGCAGAGAGCGAGACTTT893910.5971372203175888No Hit
TGGAGTGTGACAATGGTGTTT869810.581038276341513No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC784000.5237166837030457No Hit
TGAGGTAGTAGTTTGTACAGT780130.521131500583236No Hit
AAGCTGCCAGTTGAAGAACTGT739900.49425762024474934No Hit
TCCTGTACTGAGCTGCCCCG718160.4797351703675757No Hit
TAGCTTATCAGACTGATGTTGAC662910.44282784029794137No Hit
TCGTACCGTGAGTAATAATGCG624300.4170361296375146No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT582760.3892871614729425No Hit
CATTGCACTTGTCTCGGTCTGA576580.38515888455293634No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC569160.3802022802250325No Hit
TATTGCACTTGTCCCGGCCTGC566810.3786324661858716No Hit
CCCATAAAGTAGAAAGCACT528200.3528407555254448No Hit
TGAGGTAGTAGGTTGTATAG502370.33558616121415696No Hit
CGCGACCTCAGATCAGACGT492820.3292067041613967No Hit
AAACCGTTACCATTACTGAG468110.31270027654111315No Hit
TGAGGTAGTAGGTTGTATAGC455900.3045439235972175No Hit
TCAGTGCACTACAGAACTTTGC453380.30286054854245775No Hit
TTCAAGTAATTCAGGATAGGT432540.2889393040419839No Hit
TGTAAACATCCCCGACTGGAAGCT415520.2775698423626142No Hit
TTCAAGTAATCCAGGATAGGCC410920.27449701488170347No Hit
TAGCTTATCAGACTGATGTTGA400310.2674094714963855No Hit
TGAGGTAGTAGGTTGTGTGG399240.26669470510408666No Hit
AAACCGTTACCATTACTG381400.25477747852594596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT362870.24239932782566861No Hit
TGAGGTAGTAGGTTGTGTGGC362390.2420786849580953No Hit
TCCTGTACTGAGCTGCCCCGAA355250.2373091223029426No Hit
TGAGGTAGTAGTTTGTACAGTT352590.23553222641180724No Hit
AAACCGTTACCATTACTGAGA337330.225338455246873No Hit
TGAGGTAGTAGGTTGTATAGTC334640.2235415191765143No Hit
TGAGGTAGTAGGTTGTGTGGTC334360.22335447750376322No Hit
CGCGACCTCAGATC328360.21934644165909706No Hit
AAAAGCTGGGTTGAGAGGGCGA327410.21871183598369154No Hit
TATTGCACTTGTCCCGGCCTG322220.21524488497805533No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT296350.19796357042780305No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG281870.18829084392267537No Hit
TGAGGTAGTAGGTTGTAT273950.18300023660771603No Hit
CAACGGAATCCCAAAAGCAGCT269930.1803148525917897No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC266030.1777096292927567No Hit
TAGCTTATCAGACTGATGTTG261390.17461008157288152No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC251940.1682974251175323No Hit
TATTGCACTTGTCCCGGCCTGTA250820.16754925842652796No Hit
TGAGGGGCAGAGAGCGAGACTT249200.1664670887484681No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT245240.1638217850909884No Hit
TGGCTCAGTTCAGCAGGAAC243470.1626394145168119No Hit
AGAGGTAGTAGGTTGCATAGT240710.16079571802826545No Hit
CATTGCACTTGTCTCGGTCTG238740.15947974625926675No Hit
TGAGGTAGTAGATTGTATAGC237040.158344136103278No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA220230.1471149556784716No Hit
TGAGGTAGTAGATTGTATAG219510.1466339913771117No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT215200.14375488562869315No Hit
AGCTCGGTCTGAGGCCCCTCAGT213830.14283971744416105No Hit
TCCTGTACTGAGCTGCCCCGAGC210920.14089582005949794No Hit
TGAGGTAGTAGTTTGTGCTGT210510.14062193761011244No Hit
CTAGACTGAAGCTCCTTGAGG205850.13750902977075505No Hit
AAGCTGCCAGTTGAAGAACT204980.13692786457327846No Hit
AAACCGTTACCATTACTGAGTT204760.13678090325897405No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT203850.136173017822533No Hit
TGAGGTAGTAGATTGTAT199990.13359451476246442No Hit
TCCTGTACTGAGCTGCCCCA199110.13300666950524673No Hit
TGAGGTAGTAGATTGTATAGTC198970.1329131486688712No Hit
TGTAAACATCCTTGACTGGAAGCT196470.13124313373359361No Hit
TACAGTATAGATGATGTACT194380.12984700124770157No Hit
TTCAAGTAATCCAGGATAGGCA194080.12964659945546825No Hit
TGAGGTAGTAGTTTGTGCTGTC192290.1284508687618095No Hit
TGAGGGGCAGAGAGCGAGACC192100.1283239476267284No Hit
TGGCTCAGTTCAGCAGGAACAG189170.12636669012258311No Hit
AGAGGTAGTAGGTTGCATAGTT182490.12190441021552145No Hit
TTCACAGTGGCTAAGTTCCG176790.11809677616308857No Hit
ACCAATATTACTGTGCTGCTT171520.11457638467952345No Hit
TGAGGTAGTAGTTTGTGCT171130.11431586234962017No Hit
TCGTACCGTGAGTAATAATGCA164870.11013414495168514No Hit
AAACCGTTACCATTACTGAGG164740.1100473041750507No Hit
TGTAAACATCCCCGACTGGAAG164170.10966654076980742No Hit
AAACCGTTACTATTACTGAGT162730.10870461216708753No Hit
TGTAAACATCCCCGACTGGAAGC162290.10841068953847868No Hit
GTTTCCGTAGTGTAGTGGTTATC160100.10694775645517553No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT158770.10605930850960787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)