FastQCFastQC Report
Wed 8 May 2024
EGAF00002167710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15615202
Total Bases345.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8725865.588054512519274No Hit
TCAGTGCACTACAGAACTTTGT3889032.490540948493654No Hit
TGAGGTAGTAGGTTGTATAGT2122701.3593804294046277No Hit
TCCTGTACTGAGCTGCCCCGAG2114741.3542828328445575No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2008741.2864002655873423No Hit
TTCAAGTAATCCAGGATAGGCT1998631.279925805634791No Hit
AAACCGTTACCATTACTGAGC1837591.176795535530056No Hit
TGAGGTAGTAGATTGTATAGT1719481.101157705164493No Hit
TGAGGTAGTAGGTTGTATAGTT1646571.0544660261199312No Hit
TGAGGTAGTAGATTGTATAGTT1611711.032141627114398No Hit
TATTGCACTTGTCCCGGCCTGT1570261.0055969817105153No Hit
CGCGACCTCAGATCAGA1486300.9518288652301776No Hit
TCCTGTACTGAGCTGCCCCGA1387490.888550785318051No Hit
TGAGGTAGTAGTTTGTGCTGTT1305970.8363452486877851No Hit
TAGCTTATCAGACTGATGTTGAC1228370.7866500862428806No Hit
TGAGGTAGTAGTTTGTACAGT1209410.7745080723259296No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1068320.6841538137002646No Hit
TCAGTGCACTACAGAACTTTGC888370.5689135497574735No Hit
AAACCGTTACCATTACTGAGG869720.5569700603296711No Hit
TGAGGGGCAGAGAGCGAGACTTT832400.5330702734425081No Hit
TGAGGTAGTAGGTTGTGTGGTT810800.5192375993599058No Hit
TAGCTTATCAGACTGATGTTGA783210.501568919825693No Hit
TGAGGTAGTAGGTTGTGTGGT758340.4856421325833633No Hit
AAACCGTTACCATTACTGAG749890.48023073925012305No Hit
AAGCTGCCAGTTGAAGAACTGT711770.4558186311006416No Hit
CGCGACCTCAGATCAGACGT710660.4551077853491745No Hit
CCCATAAAGTAGAAAGCACT689120.44131353536124607No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT681910.4366962399845996No Hit
CGCGACCTCAGATC643460.41207279931441165No Hit
TCCTGTACTGAGCTGCCCCGAGT619520.3967415855395275No Hit
TGGCTCAGTTCAGCAGGAAC581190.37219499305868725No Hit
TCCTGTACTGAGCTGCCCCG560750.35910518480644693No Hit
TCCTGTACTGAGCTGCCCCGAGA551960.3534760549367213No Hit
CATTGCACTTGTCTCGGTCTGA534170.3420833108659113No Hit
TAGCTTATCAGACTGATGTTG522740.33476352083053423No Hit
TTCAAGTAATCCAGGATAGGCC514530.32950582387598953No Hit
TGAGGTAGTAGTTTGTACAGTT509210.32609888748157084No Hit
TTCACAGTGGCTAAGTTCCG506960.3246579839312998No Hit
TGAGGTAGTAGGTTGTATAG505440.3236845735328944No Hit
AAATGGATTTTTGGAGCAGGGAGA500430.32047616162762416No Hit
TGAGGGGCAGAGAGCGAGACT482850.30921790188817283No Hit
AAATGGATTTTTGGAAATAGGAGA480150.3074888176278475No Hit
TGAGGTAGTAGGTTGTATAGC453260.29026841919816343No Hit
TGAGGTAGTAGTTTGTGCTGT431760.2764997852733509No Hit
TATTGCACTTGTCCCGGCCTGC429170.27484114518659447No Hit
TCAGTGCACTACAGAACTTTGG406860.26055378598368434No Hit
TGAGGTAGTAGATTGTATAG382240.24478709913582933No Hit
TACAGTACTGTGATAACTGAAG377800.24194371612996105No Hit
TGAGGTAGTAGGTTGTATAGTC376250.24095109368421877No Hit
TGAGGTAGTAGATTGTATAGC359500.23022436725442297No Hit
AAATGGATTTTTGGAGCAGGGAG351250.2249410542367624No Hit
TTCAAGTAATTCAGGATAGGT347100.2222833876884846No Hit
CGCGACCTCAGATCAGAC345480.22124593713228943No Hit
TCGTACCGTGAGTAATAATGCG341000.21837693806330524No Hit
AAAAGCTGGGTTGAGAGGGCGA329180.21080739141254787No Hit
TGAGGTAGTAGATTGTATAGTC308820.19776881528653936No Hit
TGTAAACATCCCCGACTGGAAGCT307970.19722447394532583No Hit
TTCAAGTAATCCAGGATAGGCG305800.19583480252128665No Hit
TGAGGTAGTAGTTTGTGCTGTC298610.19123031517619815No Hit
AAACCGTTACCATTACTGAGTT294580.1886494968172682No Hit
TAGCTTATCAGACTGATGTTGAT292990.18763125830840996No Hit
AAATGGATTTTTGGAGCAGGGA290860.1862672029474867No Hit
AAATGGATTTTTGGAGCAGGGAGAT286000.18315485127890116No Hit
AAATGGATTTTTGGAGCAGGG278830.17856317196537067No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT269550.17262024532247486No Hit
TGTAAACATCCCCGACTGGAAG269450.17255620516468503No Hit
CATTGCACTTGTCTCGGTCTG267260.17115372570908785No Hit
TGAGGTAGTAGATTGTAT267080.17103845342506616No Hit
TACAGTATAGATGATGTACT264590.16944385349609953No Hit
AAATGGATTTTTGGAAATAGGAG263040.1684512310503572No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC262120.16786206159869083No Hit
TGAGGTAGTAGTTTGTACAGC261000.16714481183144478No Hit
TGAGGGGCAGAGAGCGAGACTT255730.16376989551592097No Hit
TGAGGTAGTAGGTTGTAT255220.16344329071119285No Hit
TGAGGTAGTAGGTTGTGTGG250690.16054227156331374No Hit
TGAGAACTGAATTCCATGGGTT249530.1597994057329518No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC248940.15942156880199182No Hit
AAACCGTTACCATTACTG237720.15223626309797336No Hit
TGTAAACATCCTTGACTGGAAGCT237190.15189685026168728No Hit
TCCTGTACTGAGCTGCCCCGAA231340.14815050103098248No Hit
TGAGATGAAGCACTGTAGCT230360.1475229074846422No Hit
TGGAGTGTGACAATGGTGTTT222560.14252777517703583No Hit
TTCAAGTAATCCAGGATAGGCA220600.14127258808435522No Hit
AAACCGTTACCATTACTGAGA196910.12610147470394553No Hit
TGAGGTAGTAGGTTGTATAGG195870.12543545706293135No Hit
TGAGGTAGTAGGTTGTGTGGTC193380.12384085713396471No Hit
TGAGATGAAGCACTGTAGCTC191550.12266892224641089No Hit
CAACGGAATCCCAAAAGCAGCT189600.1214201391695093No Hit
TATTGCACTTGTCCCGGCCTGG188730.1208629897967378No Hit
TGAGGTAGTAGGTTGTGTGGC183640.11760334576523569No Hit
TACAGTACTGTGATAACTGA183100.11725752891317064No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT182530.11689250001376865No Hit
ACCAATATTACTGTGCTGCTT182110.11662353135105136No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT174860.111980619911289No Hit
TATTGCACTTGTCCCGGCCTG173280.11096878541820977No Hit
CGCGACCTCAGATCAGACGC170910.1094510336785909No Hit
AGAGGTAGTAGGTTGCATAGT170790.1093741854892431No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC169890.10879782406913467No Hit
AAGCTGCCAGTTGAAGAACTGC168290.10777318154449747No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT166590.10668449886207043No Hit
TCAGTGCATCACAGAACTTTGT164760.10551256397451662No Hit
TACAGTACTGTGATAACTGAA163620.10478250617571262No Hit
AGCTCGGTCTGAGGCCCCTCAGT162320.10394998412444488No Hit
AAGCTGCCAGTTGAAGAACT159100.10188789104361248No Hit
TGGAGAGAAAGGCAGTTCCTGA158430.10145882198642067No Hit
GGCTGGTCCGATGGTAGT157930.10113862119747154No Hit
CGCGACCTCAGATCAGG156510.10022925095685602No Hit
TTTGGAGCAGGGAGA156350.10012678670439229No Hit
TGAGGTAGTAGTTTGTGCTG156250.10006274654660247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)