FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15917194
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT12874898.088668140879605No Hit
TCAGTGCACTACAGAACTTTGT5944463.7346155358789996No Hit
TTCAAGTAATCCAGGATAGGCT2615421.6431413727821624No Hit
AAACCGTTACCATTACTGAGC2321001.4581715847655057No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2285521.4358812237885648No Hit
TATTGCACTTGTCCCGGCCTGT2070911.3010521829412898No Hit
TGAGGTAGTAGGTTGTATAGT1991371.2510810636598386No Hit
TCCTGTACTGAGCTGCCCCGAG1858641.167693250456079No Hit
TGAGGTAGTAGGTTGTATAGTT1646761.0345793360312125No Hit
TGAGGTAGTAGATTGTATAGT1476090.9273556633160343No Hit
TAGCTTATCAGACTGATGTTGAC1434550.9012580986322087No Hit
TGAGGTAGTAGATTGTATAGTT1434530.901245533603473No Hit
TGAGGTAGTAGTTTGTGCTGTT1276640.8020509142503383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1209000.7595559870665647No Hit
TGGAGTGTGACAATGGTGTTT1183830.7437428984028215No Hit
TCCTGTACTGAGCTGCCCCGA1161460.7296889137620614No Hit
TCAGTGCACTACAGAACTTTGC1158340.7277287692793089No Hit
AAGCTGCCAGTTGAAGAACTGT1143650.7184997556730163No Hit
TAGCTTATCAGACTGATGTTGA1120910.7042133180006476No Hit
TGAGGTAGTAGTTTGTACAGT1061150.6666690121386973No Hit
CATTGCACTTGTCTCGGTCTGA926830.5822822791504584No Hit
TGAGGGGCAGAGAGCGAGACTTT906830.5697172504148658No Hit
TGAGGTAGTAGGTTGTGTGGTT833930.5239177206736313No Hit
CCCATAAAGTAGAAAGCACT829430.521090589208123No Hit
TGAGGTAGTAGGTTGTGTGGT814820.5119118357167727No Hit
CGCGACCTCAGATCAGA800040.5026262794811698No Hit
AAACCGTTACCATTACTGAG787500.4947480064639534No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC776940.4881136712915605No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT732540.4602193074985453No Hit
CGCGACCTCAGATC726390.45635556116235054No Hit
TGGCTCAGTTCAGCAGGAAC718660.4514991775560441No Hit
TCCTGTACTGAGCTGCCCCGAGT670880.4214813239067137No Hit
CGCGACCTCAGATCAGACGT669320.42050125166533747No Hit
TTCAAGTAATCCAGGATAGGCC607710.38179468064534494No Hit
TTCACAGTGGCTAAGTTCCG596360.3746640268378962No Hit
TCCTGTACTGAGCTGCCCCGAGA590840.3711960789068726No Hit
TAGCTTATCAGACTGATGTTG563520.35403224965405333No Hit
TGAGGGGCAGAGAGCGAGACT530580.3333376473265326No Hit
TCGTACCGTGAGTAATAATGCG528840.332244489826536No Hit
TATTGCACTTGTCCCGGCCTGC504170.3167455268811827No Hit
AAACCGTTACCATTACTGAGTT473260.29732627497032454No Hit
TGAGGTAGTAGTTTGTACAGTT463350.29110030323183844No Hit
TGAGGTAGTAGGTTGTATAG460330.289202983892764No Hit
TCCTGTACTGAGCTGCCCCG413630.2598636417951556No Hit
TGAGGTAGTAGTTTGTGCTGT412830.2593610406457319No Hit
TTCAAGTAATTCAGGATAGGT405120.254517222068161No Hit
AAAAGCTGGGTTGAGAGGGCGA393380.24714155020036824No Hit
TGAGATGAAGCACTGTAGCT387840.24366103724060914No Hit
TGAGGTAGTAGGTTGTATAGC371000.23308128304524023No Hit
TGAGAACTGAATTCCATGGGTT366510.23026043409409974No Hit
TGAGATGAAGCACTGTAGCTC357280.22446167333262385No Hit
TGTAAACATCCTTGACTGGAAGCT341660.2146483858901261No Hit
TGTAAACATCCCCGACTGGAAGCT330540.2076622299131367No Hit
TACAGTATAGATGATGTACT324890.20411260929533181No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC319450.20069492147925067No Hit
TGAGGTAGTAGGTTGTATAGTC318620.2001734727867236No Hit
CATTGCACTTGTCTCGGTCTG312050.19604586084708145No Hit
TACAGTACTGTGATAACTGAAG301750.18957487104825133No Hit
TGAGGTAGTAGATTGTATAG299560.18819900040170398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT294990.18532789133562108No Hit
TGTAAACATCCCCGACTGGAAG275270.17293877300232693No Hit
TGAGGTAGTAGATTGTATAGTC275180.17288223037301675No Hit
AAACCGTTACCATTACTG273650.17192100567474394No Hit
TGAGGTAGTAGATTGTATAGC269340.16921324198222376No Hit
TGAGGGGCAGAGAGCGAGACTT258180.16220195594776315No Hit
TCAGTGCATCACAGAACTTTGT255680.1606313273558141No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC247070.15522208248514155No Hit
TGAGGTAGTAGTTTGTGCTGTC245390.1541666200713518No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC241660.15182324221216378No Hit
TGAGGTAGTAGGTTGTAT238560.14987566275814693No Hit
AAGCTGCCAGTTGAAGAACTGC237800.14939819166619445No Hit
GCATTGGTGGTTCAGTGGTAGAATT237060.14893328560297753No Hit
TGAGGTAGTAGGTTGTGTGG226680.14241203568920502No Hit
TGAGGTAGTAGATTGTAT225880.14190943453978133No Hit
CTAGACTGAAGCTCCTTGAGG224890.1412874656173695No Hit
TGAAAAGAACTTTGAAGAGAG224860.1412686180742661No Hit
TGGCTCAGTTCAGCAGGAACAG219950.13818390351967816No Hit
TAGCAGCACGTAAATATTGGCG216320.13590335080416813No Hit
AGAGGTAGTAGGTTGCATAGT210180.13204588698234124No Hit
AGCTCGGTCTGAGGCCCCTCAGT208520.13100298959728707No Hit
TGAGGTAGTAGTTTGTACAGC200680.12607749833293483No Hit
AGAGGTAGTAGGTTGCATAGTT199190.1251414036921332No Hit
TGGAGTGTGACAATGGTGTTC195950.1231058690369672No Hit
CAACGGAATCCCAAAAGCAGCTG193090.12130906992777749No Hit
TTCAAGTAATCCAGGATAGGCA192880.12117713712605374No Hit
TGGAGAGAAAGGCAGTTCCTGA191450.12027873757145889No Hit
TAGCTTATCAGACTGATGTTGAT190690.11980126647950637No Hit
GGCTGGTCCGATGGTAGTGGGT182880.11489462275825753No Hit
TACAGTACTGTGATAACTGA179740.11292191324676949No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG177650.11160886774390008No Hit
AGGAAACTCTGGTG177630.11159630271516449No Hit
TGAGGTAGTAGGTTGTGTGGTC175840.11047173264332898No Hit
CAACGGAATCCCAAAAGCAGCT175650.11035236487034084No Hit
TGAGGTAGTAGGTTGTGTGGC175530.11027697469792729No Hit
TCCTGTACTGAGCTGCCCCGAA172410.10831683021517485No Hit
ACCAATATTACTGTGCTGCTT171030.10744984323241898No Hit
AACCCGTAGATCCGATCTTGT168820.106061407557136No Hit
GGCTGGTCCGATGGTAGT168720.10599858241345805No Hit
TATTGCACTTGTCCCGGCCTG165710.10410754558875138No Hit
CGCGACCTCAGATCAGAC165130.1037431597554192No Hit
TACAGTACTGTGATAACTGAA160920.10109822120657698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)