FastQCFastQC Report
Wed 8 May 2024
EGAF00002167713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9853971
Total Bases215 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT144805214.695111239925506No Hit
AAACCGTTACCATTACTGAGC2598102.6366020358696No Hit
TCAGTGCACTACAGAACTTTGT1787261.8137459507441211No Hit
CGCGACCTCAGATCAGA1661541.68616286774134No Hit
TCCTGTACTGAGCTGCCCCGAG1619591.6435911979038704No Hit
TCCTGTACTGAGCTGCCCCGA1403631.4244308208335503No Hit
CGCGACCTCAGATCAGACGT1374861.3952344694336933No Hit
TTCAAGTAATCCAGGATAGGCT1232411.2506734594611655No Hit
TATTGCACTTGTCCCGGCCTGT1140091.1569853412395876No Hit
TGAGGTAGTAGGTTGTATAGTT778920.7904630529154185No Hit
AAACCGTTACCATTACTGAG748280.7593689894155362No Hit
TGAGGGGCAGAGAGCGAGACT721830.7325270188028765No Hit
CGCGACCTCAGATC689700.6999208745388027No Hit
TGAGGTAGTAGATTGTATAGTT685060.695212112964408No Hit
TGAGGTAGTAGGTTGTGTGGT683670.6938015141306991No Hit
AAGCTGCCAGTTGAAGAACTGT673930.6839171741016895No Hit
TGAGGTAGTAGGTTGTATAGT663400.673231126821867No Hit
TGAGGTAGTAGTTTGTACAGT630670.6400160909748973No Hit
CATTGCACTTGTCTCGGTCTGA545550.5536346717480699No Hit
TGAGGTAGTAGTTTGTGCTGTT531830.539711350885851No Hit
TGAGGGGCAGAGAGCGAGACTTT515010.52264209017867No Hit
TGAGGTAGTAGATTGTATAGT509310.5168576201411593No Hit
TGAGGTAGTAGGTTGTGTGGTT508520.5160559128903465No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC482010.4891530531193972No Hit
TCCTGTACTGAGCTGCCCCG475610.48265820956850797No Hit
TGAGGTAGTAGTTTGTACAGTT439470.4459826398920801No Hit
AAATGGATTTTTGGAGCAGGGA408990.4150509474809699No Hit
CCCATAAAGTAGAAAGCACT406800.41282849320339987No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT394600.4004476976845172No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG383540.3892237961731367No Hit
TAGCTTATCAGACTGATGTTGAC349400.3545778651063617No Hit
TCAGTGCACTACAGAACTTTGC346100.3512289614004344No Hit
TCCTGTACTGAGCTGCCCCGAGA338510.34352648287680165No Hit
AAACCGTTACCATTACTGAGA325670.33049620300283006No Hit
AAAAGCTGGGTTGAGAGGGCGA311960.31658303033365937No Hit
AAACCGTTACCATTACTGAGTT305640.31016937232715625No Hit
TGAGGTAGTAGTTTGTGCTGT292330.29666212737991615No Hit
AAATGGATTTTTGGAGCAGGGAGA286420.29066454528839186No Hit
TTCAAGTAATCCAGGATAGGCC273820.2778778220475786No Hit
CGCGACCTCAGATCAGACGC273180.27722833769248967No Hit
TAGCTTATCAGACTGATGTTGA271410.27543210752294683No Hit
TATTGCACTTGTCCCGGCCTGC267030.270987198967807No Hit
TCGTACCGTGAGTAATAATGCG263190.2670902928372734No Hit
TGAGGTAGTAGGTTGTATAG259090.262929533687485No Hit
TCCTGTACTGAGCTGCCCCGAA258370.26219886378800994No Hit
TCCTGTACTGAGCTGCCCCGAGT257860.26168130594254846No Hit
AAATGGATTTTTGGAGCAGGG255510.25929648057620625No Hit
AAATGGATTTTTGGAGCAGGGAG237810.2413341788807781No Hit
AAACCGTTACCATTACTG231290.23471755701330965No Hit
TTCAAGTAATTCAGGATAGGT226180.2295318303656465No Hit
TGAGAACTGAATTCCATGGGTT221950.22523914470623063No Hit
TGGCTCAGTTCAGCAGGAAC218950.22219468679175128No Hit
CGCGACCTCAGATCAGACG217600.22082468073023556No Hit
TGTAAACATCCCCGACTGGAAGCT199650.2026086742086008No Hit
TTCACAGTGGCTAAGTTCCG196050.19895532471122554No Hit
TGAGGTAGTAGGTTGTGTGG192540.19539330895128473No Hit
CATTGCACTTGTCTCGGTCTG186280.18904054010307114No Hit
TGGAGTGTGACAATGGTGTTT185160.1879039424816655No Hit
TGAGGGGCAGAGAGCGAGACTT181440.1841288146677111No Hit
TAGCTTATCAGACTGATGTTG179820.18248480739389228No Hit
AAACCGTTACCATTACTGAGG174770.1773599699045187No Hit
TGAGGTAGTAGGTTGTATAGTC158540.1608894525871854No Hit
AAATGGATTTTTGGAAATAGGAGA155970.15828136697378142No Hit
CGCGACCTCAGATT154280.15656632234862475No Hit
TGAGGGGCAGAGAGCGAGACC152390.15464831386250275No Hit
CGCGACCTCAGATCAGAC147610.1497974775854323No Hit
TCCTGTACTGAGCTGCCCCA146100.14826510043514438No Hit
CTAGACTGAAGCTCCTTGAGG142610.14472338106130006No Hit
TGAGGTAGTAGGTTGTGTGGC141100.1431910039110121No Hit
AAGCTGCCAGTTGAAGAACT139450.14151655205804847No Hit
TGAGGTAGTAGATTGTATAG136820.1388475772863549No Hit
TGAGGTAGTAGATTGTATAGTC136440.13846194595052086No Hit
AAGCTGCCAGTTGAAGAACTGC134920.13691942060718468No Hit
CAACGGAATCCCAAAAGCAGCT134690.13668601216707457No Hit
TGTAAACATCCCCGACTGGAAG130480.13241362289375522No Hit
TTCAAGTAATCCAGGATAGGCA129270.13118569153491522No Hit
TGAGATGAAGCACTGTAGCT127250.12913575653916579No Hit
AAATGGATTTTTGGAGCAGGGAGAT125570.12743086010705734No Hit
GGCTGGTCCGATGGTAGT121080.12287432142838658No Hit
TGAGGTAGTAGGTTGTATAGC121060.12285402504229005No Hit
AAAAGCTGGGTTGAGAGGGCGT120760.12254957925084212No Hit
GGGTGCTGTAGGCTT119430.12119986957542293No Hit
TGTAAACATCCTTGACTGGAAGCT117820.11956601049465236No Hit
AAATGGATTTTTGGAAATAGGAG117760.11950512133636276No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA117230.11896726710480475No Hit
AGCTCGGTCTGAGGCCCCTCAGT116960.1186932658925016No Hit
TGAGGTAGTAGTTTGTGCT116450.11817570804704013No Hit
TGAGGTAGTAGTTTGTACAGC115390.11709999958392407No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT115180.11688688752991053No Hit
AAATGGATTTTTGGAAATAGGA115110.11681585017857268No Hit
TGAGGTAGTAGTTTGTGCTGTC110990.11263479464268769No Hit
TGAGGTAGTAGGTTGTGTGGTC110570.11220857053466059No Hit
AGACCGTTACCATTACTGAGT109370.11099078736886885No Hit
TAGCTTATCAGACTGATGTTGAT108380.10998611625709068No Hit
AAAAGCTGGGTTGAGAGGGCG107880.10947870660467744No Hit
TGAGGTAGTAGTTTGTGCTG106750.10833196079022356No Hit
TGGCTCAGTTCAGCAGGAACAG104980.10653573062068074No Hit
TATTGCACTTGTCCCGGCCTG104580.10612980289875017No Hit
AAACCGTTACCATTACTGAA103090.10461772213455874No Hit
TGAGGTAGTAGGTTGTAT102860.10438431369444866No Hit
GGCTGGTCCGATGGTAGTGGGTT99720.1011977810772936No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)