FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10615839
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10024269.442739287963956No Hit
TCAGTGCACTACAGAACTTTGT2568262.4192718069669295No Hit
AAACCGTTACCATTACTGAGC1847941.7407385323006501No Hit
CGCGACCTCAGATCAGA1764351.6619976998520796No Hit
TGAGGGGCAGAGAGCGAGACTTT1515631.4277062792681765No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1295071.2199412594708718No Hit
TCCTGTACTGAGCTGCCCCGAG1191051.1219555986107175No Hit
TCCTGTACTGAGCTGCCCCGA1162501.0950618222450434No Hit
TGAGGGGCAGAGAGCGAGACT1154031.0870831782584496No Hit
TGAGGTAGTAGGTTGTATAGT1144301.0779176285548415No Hit
TTCAAGTAATCCAGGATAGGCT1084221.0213229496038891No Hit
TGAGGTAGTAGGTTGTATAGTT993740.9360918152583134No Hit
TGAGGTAGTAGGTTGTGTGGT975710.919107759641042No Hit
TATTGCACTTGTCCCGGCCTGT809190.762247807262337No Hit
AAGCTGCCAGTTGAAGAACTGT776020.7310020432676118No Hit
CGCGACCTCAGATCAGACGT769000.7243892828442481No Hit
TGAGGTAGTAGATTGTATAGT685670.6458933674483948No Hit
TGAGGTAGTAGGTTGTGTGGTT671490.632535968188666No Hit
TGAGGTAGTAGATTGTATAGTT668160.6293991459365577No Hit
AAATGGATTTTTGGAGCAGGGAGA655050.6170496745476264No Hit
AAACCGTTACCATTACTGAG599530.5647504639058675No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT595190.5606622331028193No Hit
TCAGTGCACTACAGAACTTTGC497260.46841328320823256No Hit
TGGCTCAGTTCAGCAGGAAC482010.4540479560777061No Hit
TGAGGTAGTAGTTTGTGCTGTT472040.4446563290946669No Hit
AAATGGATTTTTGGAGCAGGG468940.4417361642353468No Hit
TGGAGTGTGACAATGGTGTTT459220.4325800344183818No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT453610.4272954780116767No Hit
AAATGGATTTTTGGAGCAGGGA421360.3969163435881045No Hit
AAAAGCTGGGTTGAGAGGGCGA406010.38245681759114847No Hit
AAATGGATTTTTGGAGCAGGGAG403170.3797815697845455No Hit
TTCACAGTGGCTAAGTTCCG396530.3735267650536147No Hit
TGAGGTAGTAGTTTGTACAGT388500.3659625960793113No Hit
TCGTACCGTGAGTAATAATGCG383690.36143163060404365No Hit
TCCTGTACTGAGCTGCCCCG382380.36019762545381484No Hit
TGAGGTAGTAGGTTGTGTGG375680.3538863014030262No Hit
TGAGGGGCAGAGAGCGAGACTT354960.3343682962787962No Hit
TAGCTTATCAGACTGATGTTGAC343390.3234694874328821No Hit
CCCATAAAGTAGAAAGCACT318520.3000422293518204No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG304870.28718408408416896No Hit
TAGCTTATCAGACTGATGTTGA283600.2671479851945757No Hit
TGAGGGGCAGAGAGCGAGACTTC259240.24420114133230542No Hit
TGAGGGGCAGAGAGCGAGACC247760.23338711146617805No Hit
TCCTGTACTGAGCTGCCCCGAGA246010.2317386313036586No Hit
AAACCGTTACCATTACTGAGA245660.23140893527115475No Hit
CGCGACCTCAGATC244440.23025970910071264No Hit
TTCAAGTAATCCAGGATAGGCC241240.22724534537496283No Hit
TGAGGTAGTAGGTTGTATAG236600.22287451797262564No Hit
CATTGCACTTGTCTCGGTCTGA226620.21347347110294343No Hit
AAATGGATTTTTGGAAATAGGAGA213900.201491375293088No Hit
TGAGGTAGTAGGTTGTATAGC211440.19917408317891785No Hit
TGAGGTAGTAGGTTGTATAGTC208230.19615029956652508No Hit
TCCTGTACTGAGCTGCCCCGAGT206700.19470905691015095No Hit
TGAGAACTGAATTCCATGGGTT205440.19352215119313698No Hit
TAGCTTATCAGACTGATGTTG201000.18933972152365913No Hit
TGAGGTAGTAGTTTGTACAGTT199040.18749342374163738No Hit
TGAGGTAGTAGGTTGTGTGGC196860.18543988845347031No Hit
AAACCGTTACCATTACTG196470.18507251287439458No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT194200.1829341986064408No Hit
TGGCTCAGTTCAGCAGGAACAG191030.1799480945406199No Hit
TCCTGTACTGAGCTGCCCCGAA190730.17966549794133085No Hit
TATTGCACTTGTCCCGGCCTGC183610.17295853865153757No Hit
AAAAGCTGGGTTGAGAGGGCGT182360.17178105282116657No Hit
AAATGGATTTTTGGAGCAGGGAGAT180530.17005721356550338No Hit
CTAGACTGAAGCTCCTTGAGG174060.163962546907503No Hit
AGCTCGGTCTGAGGCCCCTCAGT170570.16067500646910715No Hit
TGTAAACATCCCCGACTGGAAGCT163730.15423180400531694No Hit
AGAAGCCGGCGGGAGC160270.15097252322685No Hit
CGCGACCTCAGATCAGACGC158920.14970083853004928No Hit
TTCAAGTAATTCAGGATAGGT157460.14832553508017596No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC157370.14824075610038925No Hit
AAGCTGCCAGTTGAAGAACTGC153180.14429382359698562No Hit
GGCTGGTCCGATGGTAGTGGGTTATC149670.14098744338530378No Hit
TGGACGGAGGTTTGACGAGGAAT147760.13918824503649688No Hit
TGAGGTAGTAGGTTGTGTGGTC147430.13887738877727893No Hit
TGAGGTAGTAGTTTGTGCTGT147290.13874551036427737No Hit
AATCTGACTAGTTTTGCAATCTGC144660.13626808017717676No Hit
AAAAGCTGGGTTGAGAGGGCG142170.1339225284030777No Hit
TGTAAACATCCCCGACTGGAAG140120.13199145164126924No Hit
AAACCGTTACCATTACTGAGTT139460.13136973912283334No Hit
TGAGGTAGTAGATTGTATAGTC139240.13116250161668805No Hit
AAACCGTTACCATTACTGAGG137760.12976835839352877No Hit
TGGAATGTAATAGAAGTATGCC137540.12956112088738345No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC135410.12755468503243125No Hit
AAATGGATTTTTGGAAATAGGAG133710.12595330430312668No Hit
AAGCTGCCAGTTGAAGAACT132260.12458742073989629No Hit
GGCTGGTCCGATGGTAGTGGGTT131470.12384324969510181No Hit
TTCAAGTAATCCAGGATAGGCA129380.12187449338672147No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC128500.1210455433621403No Hit
TGAGGTAGTAGATTGTATAGC125050.1177956824703163No Hit
CGTGTATTTGACAAGCTGAGTTG123080.11593996480165156No Hit
TGGAATGTAATAGAAGTATGCT123030.11589286536843674No Hit
TGAGGTAGTAGGTTGTAT122580.11546897046950316No Hit
TGAGATGAAGCACTGTAGCT122010.11493203693085398No Hit
GGCTGGTCCGATGGTAGT121550.11449872214527744No Hit
TCCTGTACTGAGCTGCCCCA118450.11157855728595734No Hit
TCGTACCGTGAGTAATAATGCA117150.11035397202237149No Hit
AAAAGCTGGGTTGAGAGGGCGAT116260.10951560211114732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT114360.10772582364898338No Hit
GGCTGGTCCGATGGTAGTGGGT112910.106359940085753No Hit
TGAGGTAGTAGATTGTATAG111710.10522955368859682No Hit
CAACGGAATCCCAAAAGCAGCT110520.1041085871780836No Hit
TTCACAGTGGCTAAGTTCCA108130.10185723427041424No Hit
CGCGACCTCAGATCAGAC107520.1012826211851932No Hit
AAATGGATTTTTGGAAATAGGA106270.10010513535482217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)