FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10125639
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT141043013.92929374630085No Hit
AAACCGTTACCATTACTGAGC2665102.6320314204367743No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2104252.0781404511853525No Hit
TCCTGTACTGAGCTGCCCCGAG1763021.7411444354277297No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1513911.49512539406155No Hit
TCAGTGCACTACAGAACTTTGT1447891.4299245706863537No Hit
TCCTGTACTGAGCTGCCCCGA1431171.4134120325640682No Hit
TTCAAGTAATCCAGGATAGGCT1399081.381720205509993No Hit
CGCGACCTCAGATCAGA1313321.297024316193773No Hit
AAACCGTTACCATTACTGAGG1168971.1544654120100468No Hit
AAACCGTTACCATTACTGAG1086941.0734532408275665No Hit
TATTGCACTTGTCCCGGCCTGT966190.9542015076776883No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG960460.9485426055580295No Hit
TGAGGGGCAGAGAGCGAGACTTT931430.9198728100024107No Hit
AAGCTGCCAGTTGAAGAACTGT832560.8222295896584898No Hit
TGAGGGGCAGAGAGCGAGACT791200.7813827848296784No Hit
TGAGGTAGTAGGTTGTATAGTT790770.7809581202727057No Hit
TAGCTTATCAGACTGATGTTGAC684370.6758783322217986No Hit
TGAGGTAGTAGGTTGTATAGT643700.6357129658681294No Hit
TCCTGTACTGAGCTGCCCCG622710.6149834099359063No Hit
TGAGGTAGTAGATTGTATAGTT621840.6141242049020313No Hit
TGAGGTAGTAGGTTGTGTGGT578350.571173829128216No Hit
TGAGGTAGTAGTTTGTGCTGTT539720.5330231504401846No Hit
TCGTACCGTGAGTAATAATGCG487010.4809671764912812No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT444140.4386291077531008No Hit
TGAGGTAGTAGGTTGTGTGGTT439100.4336516441085842No Hit
TGAGGTAGTAGATTGTATAGT435590.4301851962132958No Hit
CGCGACCTCAGATCAGACGT416040.4108777727509345No Hit
AAAAGCTGGGTTGAGAGGGCGA372230.36761136753937207No Hit
TGAGGTAGTAGTTTGTACAGT364350.35982914263485No Hit
AAACCGTTACCATTACTGAGA333610.32947056477127024No Hit
TAGCTTATCAGACTGATGTTGA323770.31975265956054727No Hit
TTCAAGTAATCCAGGATAGGCC323480.319466257882589No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC321130.31714541669913376No Hit
CATTGCACTTGTCTCGGTCTGA296360.29268276303352314No Hit
AAACCGTTACCATTACTG294550.2908952215262661No Hit
TCAGTGCACTACAGAACTTTGC294410.29075695864725176No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC292500.2888706579406988No Hit
CCCATAAAGTAGAAAGCACT292030.2884064897040078No Hit
TGAGGTAGTAGGTTGTGTGG289750.2861547799600598No Hit
TGGCTCAGTTCAGCAGGAAC287330.28376480733709747No Hit
TCCTGTACTGAGCTGCCCCGAGA285330.28178962335117813No Hit
TAGCTTATCAGACTGATGTTG279600.27613072123151927No Hit
TGTAAACATCCCCGACTGGAAGCT270170.26681772873790977No Hit
TGAGGTAGTAGGTTGTATAG261430.2581861747194424No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT255290.2521223598826701No Hit
TTCACAGTGGCTAAGTTCCG238200.23524441272298963No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC234560.2316495778686165No Hit
TATTGCACTTGTCCCGGCCTGC232300.22941761996452767No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA230850.22798561157473615No Hit
TGAGAACTGAATTCCATGGGTT230680.22781772093593303No Hit
TGGAGTGTGACAATGGTGTTT224670.2218822930582455No Hit
TCCTGTACTGAGCTGCCCCGAGT217830.21512716382640149No Hit
TGAGGTAGTAGTTTGTACAGTT215140.21247054136534No Hit
TGAGGGGCAGAGAGCGAGACTT212710.21007069282244803No Hit
TCCTGTACTGAGCTGCCCCGAA212100.20946826170674268No Hit
AAACCGTTACCATTACTGAGTT205050.2025057381563771No Hit
TTCAAGTAATCCAGGATAGGCG202300.19978986017573805No Hit
CTAGACTGAAGCTCCTTGAGG191930.18954853120874643No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG188290.18595369635437328No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC185280.18298104445556473No Hit
TGAGGTAGTAGTTTGTGCTGT183820.18153916014584365No Hit
AAAAGCTGGGTTGAGAGGGCG182630.18036392567422166No Hit
AAGCTGCCAGTTGAAGAACTGC177300.1751000603517467No Hit
AAGCTGCCAGTTGAAGAACT176780.17458651251540766No Hit
TGAGGGGCAGAGAGCGAGACC171800.1696683043904686No Hit
TGAGGTAGTAGGTTGTATAGTC163440.16141203532932588No Hit
TGTAAACATCCCCGACTGGAAG162280.16026642861749268No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT160810.158814668387842No Hit
TTCAAGTAATTCAGGATAGGT159590.15760980615643122No Hit
CATTGCACTTGTCTCGGTCTG158700.15673084928269712No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT157800.15584201648903342No Hit
TTCAAGTAATCCAGGATAGGCA155430.15350142346571904No Hit
TAGCTTATCAGACTGATGTTGAT154420.1525039555528298No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT153750.15184226891754685No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG151490.149610311013458No Hit
TGAGGGGCAGAGAGCGAGACTTC150080.1482178063033849No Hit
AAAAGCTGGGTTGAGAGGGCGT149440.14758574742789074No Hit
TATTGCACTTGTCCCGGCCTG146010.1441983068920391No Hit
TCCTGTACTGAGCTGCCCCA145620.14381314601478484No Hit
TGTAAACATCCTTGACTGGAAGCT141530.13977389476357985No Hit
AAATGGATTTTTGGAGCAGGGAGA140020.13828263085421078No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT139810.13807523653568926No Hit
TGGCTCAGTTCAGCAGGAACAG139220.13749255725984305No Hit
CGCGACCTCAGATC138520.1368012428647713No Hit
AGCTCGGTCTGAGGCCCCTCAGT134340.13267310833419993No Hit
TCAGTGCACTACAGAACTTTGG132810.13116209258497166No Hit
TACAGTACTGTGATAACTGAAG132730.1310830852255349No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA132590.13094482234652055No Hit
TGAGGTAGTAGATTGTATAG132450.1308065594675062No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT130810.12918690859905238No Hit
TGAGGTAGTAGGTTGTGTGGC124580.12303421048291373No Hit
TGAGGTAGTAGATTGTATAGTC123960.12242190344727874No Hit
CAACGGAATCCCAAAAGCAGCTG123380.12184910009136213No Hit
TGAGGTAGTAGGTTGTATAGC122910.12138493185467111No Hit
TCCTGTACTGAGCTGCCCCGG119680.11819500971741143No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC115900.11446191198402392No Hit
TCGTACCGTGAGTAATAATGCA115580.11414588254627683No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT114180.1127632537561333No Hit
CAACGGAATCCCAAAAGCAGCT114100.11268424639669654No Hit
GGCTGGTCCGATGGTAGTGGGTT112170.11077819385028442No Hit
TGAGGTAGTAGTTTGTGCTGTC109920.1085561118661252No Hit
CGCGACCTCAGATCAGAC108260.10691670915781216No Hit
AAATGGATTTTTGGAGCAGGG107420.10608713188372605No Hit
AAACCGTTACCATTACTGAA104600.10330212246357984No Hit
AAAAGCTGGGTTGAGAGGGCGAT103490.10220589535139461No Hit
TGTAAACATCCCCGACTGGAAGC102320.1010504127196318No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)