FastQCFastQC Report
Wed 8 May 2024
EGAF00002167716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13432344
Total Bases321.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC9239786.878754743029214No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5744774.276818699699769No Hit
AAACCGTTACCATTACTGAGT5078073.780479415953016No Hit
TCAGTGCACTACAGAACTTTGT3874472.884433275383656No Hit
CGCGACCTCAGATCAGA3811112.8372635483427167No Hit
TTCAAGTAATCCAGGATAGGCT2316071.7242485749322678No Hit
TAGCTTATCAGACTGATGTTGAC2068041.5395972586765199No Hit
TCCTGTACTGAGCTGCCCCGAG1721691.281749484676688No Hit
TGAGGTAGTAGGTTGTATAGTT1427401.0626589074847992No Hit
TGAGGGGCAGAGAGCGAGACTTT1228210.9143675891564421No Hit
TGGAGTGTGACAATGGTGTTT1115530.8304805177711351No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1113150.8287086751202917No Hit
TGAGGTAGTAGATTGTATAGTT1069040.7958700283435266No Hit
AAGCTGCCAGTTGAAGAACTGT1043780.7770646731501218No Hit
TCCTGTACTGAGCTGCCCCGA1000340.7447248224137202No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT964430.7179908435936424No Hit
TATTGCACTTGTCCCGGCCTGT946500.7046424659761543No Hit
TGAGGTAGTAGGTTGTATAGT935610.6965351691409928No Hit
TCGTACCGTGAGTAATAATGCG903320.6724961778822818No Hit
TGAGGTAGTAGTTTGTGCTGTT863620.6429406513114911No Hit
TAGCTTATCAGACTGATGTTGA861190.641131585075546No Hit
CATTGCACTTGTCTCGGTCTGA773130.5755734069943415No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC768220.5719180509373495No Hit
TGAGGTAGTAGGTTGTGTGGTT640460.47680434628535423No Hit
TGGCTCAGTTCAGCAGGAAC630060.46906184058418987No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC618490.46044830299164463No Hit
TGAGGTAGTAGATTGTATAGT594410.44252142440664116No Hit
CGCGACCTCAGATC564400.42017982862856995No Hit
TCCTGTACTGAGCTGCCCCGAGA552070.41100049254247806No Hit
AAACCGTTACCATTACTGAGC547400.40752380969397445No Hit
CTAGACTGAAGCTCCTTGAGG537770.40035454720337715No Hit
CGCGACCTCAGATCAGACGT533790.39739154982927777No Hit
AAAAGCTGGGTTGAGAGGGCGA530970.39529213962953896No Hit
TGAGGTAGTAGGTTGTGTGGT523230.38952992865578784No Hit
TTCACAGTGGCTAAGTTCCG521710.3883983316686946No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT514380.38294135409277785No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT504520.3756008631107125No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA484700.36084543397637825No Hit
AAACCGTTACCATTACTGAG458870.3416157299128134No Hit
TGAGGGGCAGAGAGCGAGACT444980.3312750179715469No Hit
TCAGTGCACTACAGAACTTTGC442690.32957017777388664No Hit
TGTAAACATCCCCGACTGGAAGCT440450.3279025611613282No Hit
CCCATAAAGTAGAAAGCACT414260.3084048472850308No Hit
TGAGAACTGAATTCCATGGGTT378870.28205799375001117No Hit
TCCTGTACTGAGCTGCCCCG368300.2741889278595009No Hit
TCCTGTACTGAGCTGCCCCGAGT367890.27388369446166655No Hit
TGAGGTAGTAGTTTGTACAGT351380.2615924666610682No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT350730.2611085600547455No Hit
TGGCTCAGTTCAGCAGGAACAG342420.2549220002108344No Hit
TAGCTTATCAGACTGATGTTG340960.2538350715258632No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT337050.25092418717090625No Hit
TTCAAGTAATCCAGGATAGGCC324180.24134283636571546No Hit
CAACGGAATCCCAAAAGCAGCTG320450.2385659569171248No Hit
CGCGACCTCAGATCAGAC312300.23249851254553933No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC301270.22428698967209298No Hit
TGTAAACATCCCCGACTGGAAG295380.21990205134710666No Hit
TGAGATGAAGCACTGTAGCTC294450.21920969266421408No Hit
TGTAAACATCCCCGACTGGAAGC294310.21910546662592917No Hit
AGCTCGGTCTGAGGCCCCTCAGT270230.2011785880409257No Hit
TGAGGTAGTAGGTTGTATAG265620.1977465734945442No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG263850.19642885858194223No Hit
TGAGGTAGTAGTTTGTACAGTT258820.192684165920706No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA257710.19185780233144714No Hit
TGTAAACATCCTTGACTGGAAGCT256640.19106121761026965No Hit
TGAGATGAAGCACTGTAGCT253700.1888724708062867No Hit
CATTGCACTTGTCTCGGTCTG251820.18747286400646082No Hit
AAATGGATTTTTGGAGCAGGGAGA220920.16446868841357845No Hit
TGAGGTAGTAGGTTGTGTGG216070.16085800065870856No Hit
TGAGGGGCAGAGAGCGAGACTT207960.1548203351552045No Hit
CGTGTATTTGACAAGCTGAGTTG206060.1534058389213379No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC204520.152259352500204No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA191210.14235043414611775No Hit
CGCGACCTCAGATCAGACG188830.14057859149527438No Hit
CTAGACTGAAGCTCCTTGAGGA186590.13891097488271592No Hit
GGCTGGTCCGATGGTAGTGGGTTATC181810.13535240014698852No Hit
TAGCTTATCAGACTGATGTTGACT179730.13380389900675563No Hit
AACATTCAACGCTGTCGGTGA178150.1326276337175403No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA177190.13191294088358668No Hit
TCAGTGCATCACAGAACTTTGT176840.1316523757878744No Hit
AAACCGTTACCATTACTGAGTT175110.1303644397433538No Hit
TGGAGTGTGACAATGGTGTTTG171710.1278332359564347No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC168430.1253913687737598No Hit
TACAGTACTGTGATAACTGAAG165060.12288249913790177No Hit
AGAGGTAGTAGGTTGCATAGTT163780.12192957535929692No Hit
TGGCTCAGTTCAGCAGGAACA160830.1197333838382936No Hit
TGAAAAGAACTTTGAAGAGAG160050.11915269591070626No Hit
TAGCTTATCAGACTGATGTTGACA159480.11872834704054631No Hit
AAACCGTTACCATTACTG158790.11821466156614213No Hit
AAATGGATTTTTGGAGCAGGGA157220.11704584099394716No Hit
TCGTACCGTGAGTAATAATGC155570.11581746268558935No Hit
AAAAGCTGGGTTGAGAGGGCG150770.11224399851582122No Hit
TGAGGTAGTAGGTTGTGTGGTTT148740.1107327209606901No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT148720.11071783152664941No Hit
CAACGGAATCCCAAAAGCAGCT146440.10902043604600954No Hit
GGCTGGTCCGATGGTAGTG142180.10584898659534032No Hit
TTCAAGTAATTCAGGATAGGT140160.10434515375722957No Hit
TGAGGTAGTAGATTGTATAG140020.10424092771894465No Hit
GGCTGGTCCGATGGTAGTGGGT138620.10319866733609562No Hit
TGAGGTAGTAGTTTGTGCTGT138000.1027370948808339No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)