FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12141549
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5971874.918540459705759No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5706234.699754537085837No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3643723.001033887850718No Hit
TTCAAGTAATCCAGGATAGGCT2985882.459224930855198No Hit
TGGAGTGTGACAATGGTGTTT2251371.854269171091761No Hit
TCAGTGCACTACAGAACTTTGT2198111.8104032689733411No Hit
CGCGACCTCAGATCAGA1902411.5668593850751662No Hit
TGAGGTAGTAGGTTGTATAGTT1829471.50678467796819No Hit
TAGCTTATCAGACTGATGTTGAC1804031.4858318324951783No Hit
TCCTGTACTGAGCTGCCCCGAG1755311.4457051567308257No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1738381.431761301626341No Hit
TATTGCACTTGTCCCGGCCTGT1659331.3666542876860275No Hit
TCCTGTACTGAGCTGCCCCGA1457121.2001104636648914No Hit
TGAGGTAGTAGATTGTATAGTT1447231.1919648802636302No Hit
TCGTACCGTGAGTAATAATGCG1429771.1775845075451246No Hit
TGAGGTAGTAGGTTGTATAGT998800.8226297978948156No Hit
AAGCTGCCAGTTGAAGAACTGT870930.7173137463761832No Hit
TGAGGGGCAGAGAGCGAGACTTT861870.7098517660308417No Hit
AAACCGTTACCATTACTGAGC706080.5815402960528347No Hit
TAGCTTATCAGACTGATGTTGA696730.5738394664469912No Hit
CATTGCACTTGTCTCGGTCTGA673720.5548880130533592No Hit
TGAGGTAGTAGATTGTATAGT673390.5546162190672705No Hit
TCCTGTACTGAGCTGCCCCGAGA661530.5448481079308745No Hit
TGAGGTAGTAGTTTGTGCTGTT656790.5409441579488745No Hit
TGAGGTAGTAGGTTGTGTGGTT656710.5408782684977016No Hit
CTAGACTGAAGCTCCTTGAGG560840.46191799744826634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC560770.4618603441784899No Hit
TGAGGTAGTAGGTTGTGTGGT507280.41780500988794755No Hit
TGTAAACATCCCCGACTGGAAGCT497940.41011241646350066No Hit
AAAAGCTGGGTTGAGAGGGCGA466570.3842755154222909No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC450880.3713529468109876No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC450310.37088348447138003No Hit
CGCGACCTCAGATCAGACGT444090.3657605796426799No Hit
TTCAAGTAATCCAGGATAGGCC436430.3594516646928658No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT425750.3506554229612712No Hit
TCCTGTACTGAGCTGCCCCGAGT423000.3483904730771996No Hit
TCCTGTACTGAGCTGCCCCG410460.338062301605833No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA395710.3259139340458124No Hit
TGGCTCAGTTCAGCAGGAAC380590.31346082777411677No Hit
TGGCTCAGTTCAGCAGGAACAG373170.3073495811778217No Hit
TGAGGGGCAGAGAGCGAGACT356840.29389989695713453No Hit
CGCGACCTCAGATC343750.2831187355089536No Hit
TGAGAACTGAATTCCATGGGTT342740.2822868811878946No Hit
TGAGGTAGTAGTTTGTACAGTT340060.28007958457359927No Hit
TAGCTTATCAGACTGATGTTG335820.2765874436614307No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT329060.2710197850373128No Hit
TGTAAACATCCCCGACTGGAAGC328380.27045972470234236No Hit
CCCATAAAGTAGAAAGCACT328000.2701467498092706No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG325090.267750021022853No Hit
TGAGGTAGTAGTTTGTACAGT324300.2670993626925197No Hit
AAACCGTTACCATTACTGAG309170.25463802023942744No Hit
CAACGGAATCCCAAAAGCAGCTG305970.2520024421925077No Hit
TTCACAGTGGCTAAGTTCCG304850.2510799898760858No Hit
TGAGGTAGTAGGTTGTATAG291790.24032353697209474No Hit
AGCTCGGTCTGAGGCCCCTCAGT282350.2325485817336816No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT278050.2290070237331332No Hit
TCAGTGCACTACAGAACTTTGC277610.22864463175168176No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT260790.21479137464256No Hit
TGGAGTGTGACAATGGTGTTTG258270.21271585693061076No Hit
TGTAAACATCCCCGACTGGAAG224910.18523995579147273No Hit
TATTGCACTTGTCCCGGCCTGC224500.18490227235421114No Hit
TCGTACCGTGAGTAATAATGC215970.1778768096228908No Hit
TATTGCACTTGTCCCGGCCTG214430.17660843768781068No Hit
CTAGACTGAAGCTCCTTGAGGA213190.17558715119462928No Hit
AGAGGTAGTAGGTTGCATAGTT202240.1665685325653259No Hit
TGAGGTAGTAGGTTGTGTGG200900.16546488425817826No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT197420.16259869313215308No Hit
TGAGGTAGTAGGTTGTATAGTC194750.16039963269925442No Hit
CATTGCACTTGTCTCGGTCTG194600.1602760899783051No Hit
CGCGACCTCAGATCAGAC182900.15063975774425487No Hit
TTCAAGTAATTCAGGATAGGT182530.1503350190325798No Hit
TGGAGTGTGACAATGGTGTTC182410.1502361848558203No Hit
TCGTACCGTGAGTAATAATGCA181910.1498243757859891No Hit
CAACGGAATCCCAAAAGCAGCT181420.1494208028975545No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT176400.1452862398364492No Hit
TGTAAACATCCTTGACTGGAAGCT171610.14134110894746627No Hit
AACCCGTAGATCCGATCTTGT168430.1387220032633398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT164450.1354440030674834No Hit
CGCGACCTCAGATCAGACG161220.13278371647637383No Hit
TAGCTTATCAGACTGATGTTGACT160540.13222365614140338No Hit
TGAGGTAGTAGATTGTATAGTTT159990.13177066616458905No Hit
TGAGGTAGTAGGTTGTGTGGTTT158870.13084821384816717No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC157690.12987634444336552No Hit
TGAGGTAGTAGGTTGTATAGTTT156750.12910214339208284No Hit
AAACCGTTACCATTACTG154910.127586686015104No Hit
TGAGGTAGTAGATTGTATAGTC152940.12596415827996907No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA152510.12561000247991422No Hit
TAGCTTATCAGACTGATGTTGACA151430.12472049488907883No Hit
TGAGGGGCAGAGAGCGAGACTT149110.12280970080506204No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC147940.121846067581657No Hit
TGAGGTAGTAGATTGTATAG147160.12120364543272032No Hit
TTCAAGTAATTCAGGATAGGTT145890.12015765039534906No Hit
TCAGTGCATCACAGAACTTTGT144380.11891398700445883No Hit
TATTGCACTTGTCCCGGCCTGTA142010.1169620120134589No Hit
TGGCTCAGTTCAGCAGGAACA137500.11324749420358145No Hit
TACCCTGTAGAACCGAATTTGT134410.11070251415202458No Hit
TGAGATGAAGCACTGTAGCTC126750.10439359920221053No Hit
TACAGTACTGTGATAACTGAAG121430.10001195069920649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)