FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13157206
Sequences flagged as poor quality0
Sequence length14-50
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5527124.200831088302486No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5485614.16928183688847No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3791272.881516030075078No Hit
TTCAAGTAATCCAGGATAGGCT2437651.8527109783034486No Hit
TAGCTTATCAGACTGATGTTGAC2381791.8102551559958855No Hit
TCAGTGCACTACAGAACTTTGT1922381.4610852790478464No Hit
CGCGACCTCAGATCAGA1695361.2885410473925847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1514341.1509586457793546No Hit
TCCTGTACTGAGCTGCCCCGAG1349101.0253696719501084No Hit
TCGTACCGTGAGTAATAATGCG1067130.8110612541902894No Hit
TCCTGTACTGAGCTGCCCCGA971640.7384850552617326No Hit
TGAGGTAGTAGGTTGTATAGTT953010.7243255140947097No Hit
TGAGGTAGTAGATTGTATAGTT941970.7159346748846221No Hit
TAGCTTATCAGACTGATGTTGA900730.6845906342121572No Hit
TATTGCACTTGTCCCGGCCTGT858610.6525777585301925No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC782110.5944347150907267No Hit
TGAGGTAGTAGTTTGTGCTGTT639880.48633425668033164No Hit
CGCGACCTCAGATC631550.48000312528358985No Hit
AAGCTGCCAGTTGAAGAACTGT622620.47321597001673454No Hit
CATTGCACTTGTCTCGGTCTGA507550.3857581921268087No Hit
TGAGGTAGTAGGTTGTATAGT501180.3809167387057708No Hit
CGCGACCTCAGATCAGACGT488620.3713706390247291No Hit
AAACCGTTACCATTACTGAGC473130.3595976227779667No Hit
AAACCGTTACCATTACTGAG464770.3532436901877192No Hit
TCCTGTACTGAGCTGCCCCGAGA429210.32621667548566163No Hit
TAGCTTATCAGACTGATGTTG415030.31543931135531356No Hit
TGAGGTAGTAGATTGTATAGT413200.3140484385514676No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA408670.31060545833211095No Hit
TTCACAGTGGCTAAGTTCCG408250.3102862416230315No Hit
TGAGGGGCAGAGAGCGAGACTTT406810.30919178433475925No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC399000.30325587362544904No Hit
CTAGACTGAAGCTCCTTGAGG395490.30058813398528533No Hit
TGTAAACATCCCCGACTGGAAGCT385860.2932689508699643No Hit
TGGCTCAGTTCAGCAGGAAC380410.28912673404976713No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT378280.2875078493108643No Hit
TGGAGTGTGACAATGGTGTTT346230.2631485742489705No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT345700.26274575316370363No Hit
AAAAGCTGGGTTGAGAGGGCGA343200.26084565370489754No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG340020.2584287271932962No Hit
CCCATAAAGTAGAAAGCACT328860.2499466832091859No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC322450.24507482819680712No Hit
TGAGGTAGTAGGTTGTGTGGTT305760.2323897642098178No Hit
TCCTGTACTGAGCTGCCCCG295480.22457655523520723No Hit
TGAGGTAGTAGTTTGTACAGT294220.223618905107969No Hit
TGTAAACATCCCCGACTGGAAGC287350.21839743179516988No Hit
TGAGAACTGAATTCCATGGGTT285020.21662653909956261No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT282440.2146656364580748No Hit
TGAGGTAGTAGTTTGTACAGTT271780.20656361236572568No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT260480.1979751628119222No Hit
TTCAAGTAATCCAGGATAGGCC258520.19648548483621828No Hit
CAACGGAATCCCAAAAGCAGCTG246460.18731940504693778No Hit
TGAGGTAGTAGGTTGTATAG241060.18321519021591665No Hit
TGAGGTAGTAGGTTGTGTGGT239440.18198392576661032No Hit
TGGCTCAGTTCAGCAGGAACAG239400.18195352417526942No Hit
TCCTGTACTGAGCTGCCCCGAGT232370.17661044449710675No Hit
TACAGTACTGTGATAACTGAAG222660.16923045819910398No Hit
TGTAAACATCCCCGACTGGAAG217350.16519464694859987No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC210370.15988956925961334No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC199360.1515215312430314No Hit
CGCGACCTCAGATCAGAC197830.15035867037424208No Hit
TGAGGGGCAGAGAGCGAGACT195770.14879298842018587No Hit
AAACCGTTACCATTACTG195230.14838256693708377No Hit
CGGTCTGGTGGCCTGAGCGGCGTGCCCA194320.14769093073407835No Hit
CATTGCACTTGTCTCGGTCTG186360.1416410140572398No Hit
CGCGACCTCAGATCAGACG185750.14117738978929115No Hit
AGCTCGGTCTGAGGCCCCTCAGT184440.14018173767287673No Hit
TCGTACCGTGAGTAATAATGC179800.1366551530773327No Hit
TGAGATGAAGCACTGTAGCTC178920.13598631806783296No Hit
TGTAAACATCCTTGACTGGAAGCT176640.1342534273614018No Hit
TCAGTGCACTACAGAACTTTGC173270.1316920932909312No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT166270.1263718148062742No Hit
TTCAAGTAATTCAGGATAGGT158230.12026109494675388No Hit
TCAGTGCATCACAGAACTTTGT157390.11962266152859505No Hit
TAGCTTATCAGACTGATGTTGACA155790.11840659787495916No Hit
CTAGACTGAAGCTCCTTGAGGA154220.11721333541482896No Hit
GTTCTATTTTGTTGGTTTTCGGAAC153830.1169169198992552No Hit
TGAGGTAGTAGATTGTATAG152570.11595926977201695No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC151780.11535883834303423No Hit
TAGCTTATCAGACTGATGTTGACT150260.11420357787208014No Hit
GTTTCCGTAGTGTAGTGGTTATC145870.11086700322241666No Hit
TATTGCACTTGTCCCGGCCTG138570.10531871280270294No Hit
TGGAGAGAAAGGCAGTTCCTGA138220.1050526988784701No Hit
TGAAAAGAACTTTGAAGAGAG136770.10395064119236258No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA136500.10374543045081153No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC135520.10300059146295955No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC134920.10254456759284608No Hit
CAACGGAATCCCAAAAGCAGCT132780.10091808245610807No Hit
TGAGGTAGTAGTTTGTGCTGT132400.10062926733836956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)