FastQCFastQC Report
Wed 8 May 2024
EGAF00002167719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13818125
Total Bases305.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT155044411.220364557420055No Hit
TCAGTGCACTACAGAACTTTGT4180533.0253959925822063No Hit
TGAGGTAGTAGGTTGTATAGT3392532.455130489845764No Hit
AAACCGTTACCATTACTGAGC2627971.9018282147541727No Hit
TGAGGTAGTAGGTTGTATAGTT2374141.718134696277534No Hit
TGAGGTAGTAGATTGTATAGT2112861.529049708263603No Hit
TTCAAGTAATCCAGGATAGGCT1774951.2845085711701116No Hit
TGAGGTAGTAGATTGTATAGTT1694341.2261721470894205No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1195330.8650450042968928No Hit
TGGAGTGTGACAATGGTGTTT1112660.8052177846126012No Hit
TCCTGTACTGAGCTGCCCCGAG1092050.7903025917047356No Hit
AAGCTGCCAGTTGAAGAACTGT1082620.7834782215387399No Hit
TGAGGTAGTAGGTTGTGTGGTT959780.6945804875842416No Hit
TGAGGTAGTAGGTTGTGTGGT948780.6866199285358904No Hit
TATTGCACTTGTCCCGGCCTGT927530.671241575828848No Hit
TGAGGTAGTAGTTTGTACAGT926240.6703080193586323No Hit
TCCTGTACTGAGCTGCCCCGA922340.6674856393323986No Hit
CGCGACCTCAGATCAGA769390.5567976842010042No Hit
CCCATAAAGTAGAAAGCACT768000.555791759012167No Hit
CGCGACCTCAGATCAGACGT764730.553425301913248No Hit
TGAGGTAGTAGTTTGTGCTGTT764670.5533818806820752No Hit
TCAGTGCACTACAGAACTTTGC750340.543011443303632No Hit
TGAGGTAGTAGGTTGTATAG681420.49313492242977974No Hit
TGGCTCAGTTCAGCAGGAAC667870.48332896105658335No Hit
TTCACAGTGGCTAAGTTCCG645220.46693744628884165No Hit
AAAAGCTGGGTTGAGAGGGCGA644890.4666986295173911No Hit
TAGCTTATCAGACTGATGTTGAC641210.4640354606721245No Hit
TGAGGGGCAGAGAGCGAGACTTT622810.45071961644579134No Hit
TAGCTTATCAGACTGATGTTGA608210.44015378352707046No Hit
TGAGGTAGTAGGTTGTATAGC592020.42843728798226965No Hit
AAACCGTTACCATTACTGAG556590.4027970509747162No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT553780.4007634899814555No Hit
TCCTGTACTGAGCTGCCCCGAGT511160.3699199421050251No Hit
TAGCTTATCAGACTGATGTTG498210.3605481930435569No Hit
TGAGGTAGTAGTTTGTACAGTT460970.33359808222895654No Hit
TGGAGAGAAAGGCAGTTCCTGA453790.3284020082319417No Hit
TGAGGTAGTAGGTTGTATAGTC449830.3255362069745353No Hit
TCGTACCGTGAGTAATAATGCG439590.3181256501877064No Hit
TTCAAGTAATTCAGGATAGGT427190.30915192907865574No Hit
TCCTGTACTGAGCTGCCCCGAGA426990.309007191641413No Hit
TGAGGGGCAGAGAGCGAGACT412680.29865122800669414No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT412360.2984196481071057No Hit
TTCAAGTAATCCAGGATAGGCC372150.26932018634945043No Hit
TGAGGTAGTAGATTGTATAG367430.2659043828305215No Hit
ACGGCCCTGGCGGAGC363370.2629662128544936No Hit
TGAGGTAGTAGATTGTATAGC360270.260722782577231No Hit
AGAGGTAGTAGGTTGCATAGT359120.25989054231308517No Hit
AAACCGTTACCATTACTG337020.2438970554977611No Hit
TGAGGTAGTAGGTTGTAT329110.23817268985481024No Hit
TGAGGTAGTAGATTGTATAGTC316620.22913383689900038No Hit
CATTGCACTTGTCTCGGTCTGA310600.22477724003799357No Hit
TACAGTATAGATGATGTACT301300.2180469492062056No Hit
TGAGGTAGTAGTTTGTGCTGT294730.2132923243927812No Hit
TGAGATGAAGCACTGTAGCT286540.20736532633769053No Hit
TCCTGTACTGAGCTGCCCCG286350.20722782577230991No Hit
AGAGGTAGTAGGTTGCATAGTT285260.20643900673933693No Hit
TGAGAACTGAATTCCATGGGTT283960.20549821339725904No Hit
AAACCGTTACCATTACTGAGA281840.20396399656248584No Hit
TGAGGTAGTAGGTTGTGTGG271350.19637251797910352No Hit
TGAGGGGCAGAGAGCGAGACTT239260.173149396173504No Hit
TGAGGTAGTAGATTGTAT235300.1702835949160975No Hit
AAGCTGCCAGTTGAAGAACT226460.16388620018996788No Hit
CAACGGAATCCCAAAAGCAGCT221690.16043421231172825No Hit
AAACCGTTACCATTACTGAGG204860.1482545569677507No Hit
AAGCTGCCAGTTGAAGAACTGC200050.1447736216020625No Hit
TGTAAACATCCCCGACTGGAAGCT199190.1441512506219187No Hit
TATTGCACTTGTCCCGGCCTGC197840.1431742729205301No Hit
CGCGACCTCAGATC191380.13849925369758923No Hit
TGAGGTAGTAGGTTGTGTGGTC189970.13747885476502783No Hit
TGGCTCAGTTCAGCAGGAACAG188350.13630648152336153No Hit
TGGAGTGTGACAATGGTGTTC185960.13457686914831066No Hit
TGAGGTAGTAGGTTGTGTGGC180530.13064724772716993No Hit
ACGGCCCTGGCGGA176690.12786828893210908No Hit
AAAAGCTGGGTTGAGAGGGCGAT173500.1255597268080872No Hit
ACCAATATTACTGTGCTGCTT170220.12318603283730607No Hit
AAAAGCTGGGTTGAGAGGGCGT167970.1215577366683251No Hit
GGCTGGTCCGATGGTAGT167890.12149984169342802No Hit
AAAAGCTGGGTTGAGAGGGCG167660.12133339364059886No Hit
TGTAAACATCCCCGACTGGAAG167090.120920891944457No Hit
TTCAAGTAATCCAGGATAGGCA164290.11889456782305849No Hit
AGCTCGGTCTGAGGCCCCTCAGT163810.11854719797367587No Hit
TGTAAACATCCTTGACTGGAAGCT161920.11717942919173188No Hit
TGAGGTAGTAGTTTGTACAGC161050.11654982133972591No Hit
GGCTGGTCCGATGGTAGTGGGT158700.11484915645212357No Hit
AGAAGCCGGCGGGAGC158010.11434981229363607No Hit
AAATGGATTTTTGGAAATAGGAGA153500.11108598308381201No Hit
TGAGATGAAGCACTGTAGCTC152600.11043466461621965No Hit
TTCAAGTAATTCAGGATAGGTT148950.10779320638653943No Hit
CGCGACCTCAGATCAGACGC147860.10700438735356643No Hit
TGGAGAGAAAGGCAGTTCCTG147570.10679451806956443No Hit
AAATGGATTTTTGGAGCAGGGAGAT146680.10615043647383418No Hit
CATTGCACTTGTCTCGGTCTG144660.1046885883576824No Hit
CCCATAAAGTAGAAAGCACC143590.10391424306843368No Hit
TGAGGTAGTAGTTTGTGCTGTC142890.10340766203808403No Hit
AAACCGTTACCATTACTGAGTT141950.1027273960830431No Hit
AAATGGATTTTTGGAGCAGGGAGA141650.10251028992717898No Hit
TACAGTACTGTGATAACTGAAG141320.10227147315572845No Hit
AAATGGATTTTTGGAGCAGGGA139250.10077344068026596No Hit
CTAGACTGAAGCTCCTTGAGG138440.10018725405943282No Hit
TTCACAGTGGCTAAGTTCCA138340.10011488534081142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)