FastQCFastQC Report
Fri 10 May 2024
EGAF00002167720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18105208
Total Bases407 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT207209911.444767715455132No Hit
TCAGTGCACTACAGAACTTTGT4504812.4881293824406767No Hit
AAACCGTTACCATTACTGAGC3230671.784387122202628No Hit
TGAGGTAGTAGGTTGTATAGT3093271.7084973561198524No Hit
TCCTGTACTGAGCTGCCCCGAG2344031.2946716767904571No Hit
TATTGCACTTGTCCCGGCCTGT2176871.202344651329054No Hit
TTCAAGTAATCCAGGATAGGCT2030101.1212795787819725No Hit
TGAGGTAGTAGATTGTATAGT1993051.1008158536482984No Hit
TGAGGTAGTAGGTTGTATAGTT1956261.0804957336032814No Hit
TGAGGTAGTAGGTTGTGTGGT1885561.04144619603376No Hit
AAACCGTTACCATTACTGAGG1756090.9699363851550338No Hit
TGGAGTGTGACAATGGTGTTT1754400.9690029520787611No Hit
CGCGACCTCAGATCAGA1751970.9676607968270787No Hit
TGAGGTAGTAGGTTGTGTGGTT1674360.9247946778628557No Hit
TCCTGTACTGAGCTGCCCCGA1660150.9169461074404669No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1502810.8300429357121994No Hit
TGAGGTAGTAGATTGTATAGTT1378460.7613610404254952No Hit
AAGCTGCCAGTTGAAGAACTGT1378070.7611456327925092No Hit
TGAGGTAGTAGTTTGTACAGT971630.5366577395852067No Hit
TGAGGTAGTAGTTTGTGCTGTT962160.5314272003944942No Hit
TGAGGGGCAGAGAGCGAGACTTT946770.5229268838005064No Hit
TCGTACCGTGAGTAATAATGCG936330.5171605871636492No Hit
TGAGGGGCAGAGAGCGAGACT915710.5057715989785923No Hit
TCCTGTACTGAGCTGCCCCGAGT887490.4901849235866277No Hit
CATTGCACTTGTCTCGGTCTGA875840.4837503109602496No Hit
TCCTGTACTGAGCTGCCCCGAGA815440.45038974421061606No Hit
CCCATAAAGTAGAAAGCACT783290.43263242267086904No Hit
TAGCTTATCAGACTGATGTTGAC753400.416123360747913No Hit
TAGCTTATCAGACTGATGTTG748600.41347218988039247No Hit
TAGCTTATCAGACTGATGTTGA746660.41240067498810284No Hit
TCAGTGCACTACAGAACTTTGC745380.4116936960900974No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT732960.40483379147038795No Hit
TGAGGTAGTAGGTTGTATAG728900.40259134277827685No Hit
TCCTGTACTGAGCTGCCCCG680230.3757095748361466No Hit
TGAGGTAGTAGGTTGTGTGG667050.36842990149574645No Hit
TGGCTCAGTTCAGCAGGAAC662160.3657290211744599No Hit
AAACCGTTACCATTACTGAG661690.36546942736034843No Hit
AAAAGCTGGGTTGAGAGGGCGA661460.3653423920896131No Hit
TTCACAGTGGCTAAGTTCCG627200.3464196600226852No Hit
CGCGACCTCAGATCAGACGT539720.29810207096212316No Hit
AAACCGTTACCATTACTG489970.2706237895748008No Hit
CTAGACTGAAGCTCCTTGAGG487090.26903308705428847No Hit
TGAGGTAGTAGGTTGTATAGC480800.2655589485633084No Hit
TTCAAGTAATTCAGGATAGGT443410.24490743215985147No Hit
TGAGGTAGTAGTTTGTACAGTT432760.23902514679754022No Hit
TATTGCACTTGTCCCGGCCTGC425990.2352858912198081No Hit
TGGCTCAGTTCAGCAGGAACAG421860.23300477961921232No Hit
CATTGCACTTGTCTCGGTCTG405370.2238969030347511No Hit
AAACCGTTACCATTACTGAGA404090.22318992413674563No Hit
TCAGTGCACTACAGAACTTTGG398980.22036753181736438No Hit
TTCAAGTAATCCAGGATAGGCC390360.21560647080110873No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT389440.21509832971816728No Hit
TGAGGTAGTAGATTGTATAG374290.20673057166755554No Hit
TGAGGTAGTAGGTTGTATAGTC333340.18411277020402086No Hit
TGAGGTAGTAGGTTGTGTGGC328470.18142293642801563No Hit
TGAGAACTGAATTCCATGGGTT319640.17654588668630594No Hit
TGAGGTAGTAGATTGTATAGC305470.16871940935447965No Hit
TGTAAACATCCCCGACTGGAAG305370.16866417662807298No Hit
TGAGGTAGTAGGTTGTGTGGTC300220.16581969121812906No Hit
TGAGGTAGTAGGTTGTAT293100.1618871210979736No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC292900.16177665564516022No Hit
TTCAAGTAATCCAGGATAGGCG288180.15916967095876502No Hit
TGAGGGGCAGAGAGCGAGACTT287830.15897635641634164No Hit
AAGCTGCCAGTTGAAGAACT283450.15655716299972913No Hit
TGTAAACATCCCCGACTGGAAGCT277670.15336471141342314No Hit
TGAGGTAGTAGTTTGTGCTGT269460.14883010457543486No Hit
AAAAGCTGGGTTGAGAGGGCG261020.14416846246671125No Hit
TGAGATGAAGCACTGTAGCT256090.14144548905486198No Hit
TGGAGTGTGACAATGGTGTTC255490.14111409269642194No Hit
AGAGGTAGTAGGTTGCATAGT250380.13829170037704067No Hit
AAACCGTTACCATTACTGAGTT249020.13754053529790985No Hit
TGAGGTAGTAGATTGTAT240310.1327297648278882No Hit
TCCTGTACTGAGCTGCCCCGAA238390.13166929648088No Hit
AAGCTGCCAGTTGAAGAACTGC236780.13078004958573247No Hit
AAAAGCTGGGTTGAGAGGGCGT236520.1306364444970751No Hit
TGAGGTAGTAGGTTGTATAGG235040.12981900014625625No Hit
TGAGGTAGTAGATTGTATAGTC227440.12562131293934872No Hit
TATTGCACTTGTCCCGGCCTGG221080.122108511539884No Hit
TACAGTATAGATGATGTACT215770.11917565376768939No Hit
CGTGTATTTGACAAGCTGAGTTG213040.11766780033678706No Hit
TATTGCACTTGTCCCGGCCTG210870.11646925017376217No Hit
TGGAGAGAAAGGCAGTTCCTGA201320.1111945248019244No Hit
TGGCTCAGTTCAGCAGGAACA200610.11080237244443698No Hit
TAGCAGCACGTAAATATTGGCG199970.11044888299543425No Hit
ACCAATATTACTGTGCTGCTT198380.10957068264556807No Hit
AACCCGTAGATCCGATCTTGT198160.10944917064747336No Hit
CAACGGAATCCCAAAAGCAGCT196990.10880294774851522No Hit
TGTAAACATCCTTGACTGGAAGCT195850.1081732946674791No Hit
TACAGTACTGTGATAACTGAAG193900.10709625650254888No Hit
AGAGGTAGTAGGTTGCATAGTT192480.10631195178757405No Hit
TTCAAGTAATCCAGGATAGGCA191060.10552764707259922No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG184450.1018767638571178No Hit
TCGTACCGTGAGTAATAATGCA183010.10108141259686163No Hit
TGAGATGAAGCACTGTAGCTC182320.10070030678465554No Hit
TGAGGTAGTAGGTTGTGTGGG181110.10003199079513475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)