FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002167722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23831936
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT15802766.630917437844748No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT6562502.753657948728966No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5130992.15298916546268No Hit
TCAGTGCACTACAGAACTTTGT4104811.7223988852605177No Hit
TTCAAGTAATCCAGGATAGGCT3780741.5864174861832459No Hit
TATTGCACTTGTCCCGGCCTGT3492521.4654789271001734No Hit
AAACCGTTACCATTACTGAGC2963511.243503675068614No Hit
CGCGACCTCAGATCAGA2905791.2192840732704218No Hit
TCCTGTACTGAGCTGCCCCGAG2712411.1381408543561045No Hit
TCCTGTACTGAGCTGCCCCGA2577601.081573901507624No Hit
TAGCTTATCAGACTGATGTTGAC2181260.9152676475801211No Hit
TGAGGTAGTAGGTTGTATAGTT1860070.7804947109626343No Hit
AAGCTGCCAGTTGAAGAACTGT1843080.773365621659944No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1732020.7267642880544828No Hit
TGAGGTAGTAGATTGTATAGTT1476580.6195803815518807No Hit
TGAGGGGCAGAGAGCGAGACTTT1448200.6076719910627487No Hit
CGCGACCTCAGATCAGACGT1386650.5818453020350508No Hit
TGAGGGGCAGAGAGCGAGACT1372550.5759288712423531No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1346730.5650946696063636No Hit
TGAGGTAGTAGGTTGTATAGT1320860.554239487719336No Hit
TCGTACCGTGAGTAATAATGCG1230390.5162778214912964No Hit
TGAGGTAGTAGGTTGTGTGGT1186310.49778163217625293No Hit
TGAGGTAGTAGGTTGTGTGGTT1164260.48852934146852356No Hit
TGAGGTAGTAGTTTGTGCTGTT1116760.46859810298248533No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1097770.4606298036382776No Hit
CATTGCACTTGTCTCGGTCTGA1074910.4510376328637338No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1057850.4438791712095903No Hit
TGGAGTGTGACAATGGTGTTT925790.38846613216819653No Hit
TAGCTTATCAGACTGATGTTGA900890.37801796715130487No Hit
TGAGGTAGTAGATTGTATAGT893100.3747492440395946No Hit
TCCTGTACTGAGCTGCCCCGAGA828740.3477434649035647No Hit
TGTAAACATCCCCGACTGGAAGCT822480.34511673747361526No Hit
TCCTGTACTGAGCTGCCCCGAGT810570.34011924167637914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT789450.3312571836379554No Hit
TCCTGTACTGAGCTGCCCCG784830.3293186084420502No Hit
TGAGGTAGTAGTTTGTACAGT734310.30812016279332066No Hit
TTCAAGTAATCCAGGATAGGCC721520.3027534145778169No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT721170.3026065528205514No Hit
TATTGCACTTGTCCCGGCCTGC718370.30143165876242706No Hit
CTAGACTGAAGCTCCTTGAGG686890.2882224927089432No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT674560.28304876280298835No Hit
CCCATAAAGTAGAAAGCACT663340.2783407944700758No Hit
TCAGTGCACTACAGAACTTTGC646080.2710984118117806No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT638920.26809403986314834No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT621900.26095236240983527No Hit
TGAGGTAGTAGTTTGTACAGTT555780.23320807843727007No Hit
AAAAGCTGGGTTGAGAGGGCGA538700.22604122468271146No Hit
CGCGACCTCAGATC537920.22571393276651966No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC516960.21691901153141732No Hit
TAGCTTATCAGACTGATGTTG514880.21604623308823923No Hit
TGGCTCAGTTCAGCAGGAAC474410.19906481789813468No Hit
TGAGAACTGAATTCCATGGGTT473060.1984983511201104No Hit
AAACCGTTACCATTACTGAG469020.19680314683624528No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG462840.1942099878079565No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT449050.18842363457169403No Hit
TGGCTCAGTTCAGCAGGAACAG430990.18084556789679193No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC406640.1706281856413176No Hit
TTCAAGTAATTCAGGATAGGT406330.17049810808488242No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT397650.1668559365046969No Hit
TATTGCACTTGTCCCGGCCTG395170.1658153160532153No Hit
TGAGGTAGTAGGTTGTATAG382570.1605282927916557No Hit
AGCTCGGTCTGAGGCCCCTCAGT376970.15817850467540698No Hit
AAGCTGCCAGTTGAAGAACT369400.15500209466826362No Hit
TAGCTTATCAGACTGATGTTGAT361790.15180890046028991No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA355940.14935421108885152No Hit
TGAGGGGCAGAGAGCGAGACTT353400.14828841433612444No Hit
TGAGGTAGTAGGTTGTGTGG342420.1436811512081939No Hit
TGTAAACATCCTTGACTGGAAGCT330800.13880534086697782No Hit
CATTGCACTTGTCTCGGTCTG321610.13494917072620538No Hit
TTCACAGTGGCTAAGTTCCG319790.13418548958842455No Hit
AAACCGTTACCATTACTG317290.13313647703652778No Hit
TGTAAACATCCCCGACTGGAAGC314730.13206228818338553No Hit
AAGCTGCCAGTTGAAGAACTGC305450.12816835359074477No Hit
TGAGGTAGTAGGTTGTATAGTC294750.12367857986862671No Hit
CAACGGAATCCCAAAAGCAGCT292380.12268411596942858No Hit
TGAGGTAGTAGTTTGTGCTGT286640.12027558315027365No Hit
TCCTGTACTGAGCTGCCCCGAA276520.1160291803401956No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC276080.11584455413106177No Hit
TGTAAACATCCCCGACTGGAAG275150.11545432146175619No Hit
TAGCTTATCAGACTGATGTTGACT271840.11406542884304488No Hit
CAACGGAATCCCAAAAGCAGCTG265600.1114470935135106No Hit
TCGTACCGTGAGTAATAATGCA262570.11017569030061175No Hit
GGCTGGTCCGATGGTAGTGGGTT258610.10851405441840731No Hit
AACCCGTAGATCCGATCTTGT258600.10850985836819971No Hit
TCAGTGCATCACAGAACTTTGT255710.10729719985820708No Hit
CTAGACTGAAGCTCCTTGAGGA255450.1071881025528098No Hit
TTCAAGTAATCCAGGATAGGCA247310.103772517683834No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT242900.10192205954228813No Hit
AGAGGTAGTAGGTTGCATAGTT240450.10089402724142932No Hit
CGCGACCTCAGATCAGAC239070.1003149723127823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)