FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002167723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24008464
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT14827086.175772011070762No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC10559184.3981072675036605No Hit
AAACCGTTACCATTACTGAGT7158872.9818109146840883No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC5406902.252080766183126No Hit
TTCAAGTAATCCAGGATAGGCT3409321.42004919598355No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC3106181.2937853916851991No Hit
TCAGTGCACTACAGAACTTTGT2817501.1735444633192693No Hit
TATTGCACTTGTCCCGGCCTGT2798771.1657430479517557No Hit
TAGCTTATCAGACTGATGTTGAC2433391.0135550529180042No Hit
TCCTGTACTGAGCTGCCCCGAG2381750.992045971787283No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC2120030.883034416529104No Hit
TCCTGTACTGAGCTGCCCCGA2076780.8650199363024641No Hit
TGAGGTAGTAGGTTGTATAGTT2035650.8478884779967598No Hit
CGCGACCTCAGATCAGA1967910.8196734285042142No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1899210.7910585200286032No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1842010.7672335889542955No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT1656440.6899400144882238No Hit
TGAGGTAGTAGATTGTATAGTT1574230.6556979238655167No Hit
TCGTACCGTGAGTAATAATGCG1417500.5904167796823653No Hit
TGAGGGGCAGAGAGCGAGACTTT1416230.5898877995693519No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT1369040.5702322314330479No Hit
AAGCTGCCAGTTGAAGAACTGT1361050.5669042384385773No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1285130.5352820571944961No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1227330.5112072142557724No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT1101660.4588631742538798No Hit
AAACCGTTACCATTACTGAGC1084520.451724025327068No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT1003400.4179359412580496No Hit
TGAGGTAGTAGTTTGTGCTGTT945710.393906915494469No Hit
TGAGGGGCAGAGAGCGAGACT933630.3888753566242305No Hit
TGAGGTAGTAGGTTGTATAGT908370.37835406713232467No Hit
CTAGACTGAAGCTCCTTGAGG905120.37700037786673896No Hit
TGTAAACATCCCCGACTGGAAGCT887890.3698237421602648No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG866220.3607977586571136No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA852240.35497481221622507No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC763050.317825413570814No Hit
TGAGGTAGTAGGTTGTGTGGTT755200.3145557333447071No Hit
TGGAGTGTGACAATGGTGTTT749910.3123523437401077No Hit
TAGCTTATCAGACTGATGTTGA744330.31002816340104056No Hit
TCCTGTACTGAGCTGCCCCG717110.29869049515204305No Hit
TGAGGTAGTAGGTTGTGTGGT638140.2657979285971814No Hit
TCCTGTACTGAGCTGCCCCGAGT624810.26024572000941No Hit
CGCGACCTCAGATCAGACGT621030.2586712752635904No Hit
TCCTGTACTGAGCTGCCCCGAGA611350.2546393638510152No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT603210.2512488928904406No Hit
TTCAAGTAATCCAGGATAGGCC596360.24839573243836008No Hit
TGAGGTAGTAGATTGTATAGT578130.2408025769578595No Hit
TGGCTCAGTTCAGCAGGAACAG546140.22747810938675625No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC536080.22328792046005108No Hit
CATTGCACTTGTCTCGGTCTGA533840.22235491616623201No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC530300.22088043616617872No Hit
TATTGCACTTGTCCCGGCCTGC510490.21262917944271653No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG500380.20841816452731002No Hit
TGAGGTAGTAGTTTGTACAGTT479220.19960460610891226No Hit
AAAAGCTGGGTTGAGAGGGCGA478990.1995088065608862No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT466540.194323135374258No Hit
TGAGAACTGAATTCCATGGGTT440530.1834894560518324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG430830.17944921424377672No Hit
TCAGTGCACTACAGAACTTTGC415630.17311811367857602No Hit
AAACCGTTACCATTACTGAGG415180.17293067978026416No Hit
TGAGGTAGTAGTTTGTACAGT414870.172801558650316No Hit
TAGCTTATCAGACTGATGTTG410450.17096054124911947No Hit
TGGCTCAGTTCAGCAGGAAC409100.1703982395541839No Hit
AGCTCGGTCTGAGGCCCCTCAGT393610.1639463482545156No Hit
TGTAAACATCCCCGACTGGAAGC377990.15744030938422382No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAACT358620.14937232136133322No Hit
TATTGCACTTGTCCCGGCCTG354490.14765209469460439No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC331020.1378763755982057No Hit
CCCATAAAGTAGAAAGCACT329390.13719744836654274No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT313000.1303706892702507No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCC306760.1277716058803262No Hit
TTCAAGTAATCCAGGATAGGCG306590.12770079751874172No Hit
CAACGGAATCCCAAAAGCAGCTG303530.12642624701022107No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA294820.12279835977845145No Hit
CTAGACTGAAGCTCCTTGAGGA293450.12222772768803536No Hit
TAGCTTATCAGACTGATGTTGAT289800.12070743051283914No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA289510.12058663977837149No Hit
TGAGGTAGTAGGTTGTATAG289030.12038671028683885No Hit
TGAGGTAGTAGGTTGTGTGG288420.12013263322468276No Hit
TAGCTTATCAGACTGATGTTGACT287420.1197161134506564No Hit
TGAGGTAGTAGGTTGTATAGTC270660.11273524203797461No Hit
TGAGGGGCAGAGAGCGAGACTT269740.11235204384587036No Hit
GGCTGGTCCGATGGTAGTGGGTT266440.11097752859158337No Hit
TTCACAGTGGCTAAGTTCCG265670.11065680836558307No Hit
TGTAAACATCCTTGACTGGAAGCT265240.11047770486275173No Hit
AGAGGTAGTAGGTTGCATAGTT264370.11011533265934881No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGT255790.10654159299820264No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC254160.10586266576653967No Hit
TGCTGGTCCGATGGTAGTGGGTTATCAGAAC253160.10544614599251331No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG251150.10460894124672032No Hit
TGTAAACATCCCCGACTGGAAG249280.10383004926929104No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC242110.10084360248952204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)