FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344629
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6550515.774106848271548No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2972852.6204911592966154No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2861672.522488835906401No Hit
TTCAAGTAATCCAGGATAGGCT1934161.7049125185142677No Hit
TATTGCACTTGTCCCGGCCTGT1905501.6796494623138405No Hit
TCAGTGCACTACAGAACTTTGT1746421.539424515336729No Hit
CGCGACCTCAGATCAGA1580451.3931262097685169No Hit
TCCTGTACTGAGCTGCCCCGAG1312001.1564944080586506No Hit
TAGCTTATCAGACTGATGTTGAC1302771.1483583993800062No Hit
TCCTGTACTGAGCTGCCCCGA1287501.1348982853471894No Hit
AAACCGTTACCATTACTGAGC1241241.0941212797703654No Hit
TGAGGTAGTAGGTTGTATAGTT1085640.9569638636926778No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC955020.8418256780367167No Hit
TGAGGGGCAGAGAGCGAGACTTT950620.8379471906926176No Hit
TGAGGTAGTAGATTGTATAGTT905730.7983778050388426No Hit
AAGCTGCCAGTTGAAGAACTGT819760.7225974511815239No Hit
TGAGGTAGTAGGTTGTATAGT769670.6784443986665407No Hit
TAGCTTATCAGACTGATGTTGA601940.530594698160689No Hit
TGAGGTAGTAGTTTGTGCTGTT565970.4988880641226786No Hit
TGAGGTAGTAGGTTGTGTGGTT559930.49356395876850623No Hit
TCGTACCGTGAGTAATAATGCG536200.47264657134226246No Hit
TGAGGTAGTAGGTTGTGTGGT535030.47161524629849066No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC529070.4663616588960291No Hit
TGAGGTAGTAGATTGTATAGT522650.46070259327123No Hit
TGAGGGGCAGAGAGCGAGACT518100.45669188476767286No Hit
TGTAAACATCCCCGACTGGAAGCT511540.45090941272737967No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC496670.4378018884531173No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT419280.36958458491679186No Hit
TATTGCACTTGTCCCGGCCTGC406690.35848682226629003No Hit
TTCAAGTAATCCAGGATAGGCC405720.35763179210179546No Hit
TCCTGTACTGAGCTGCCCCGAGA395270.34842038465956005No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT394500.3477416493743427No Hit
TCCTGTACTGAGCTGCCCCGAGT384670.3390767560578667No Hit
TCCTGTACTGAGCTGCCCCG369340.32556375356126677No Hit
TGAGGTAGTAGTTTGTACAGT358900.31636116086299515No Hit
TGGAGTGTGACAATGGTGTTT357340.31498606080463276No Hit
CTAGACTGAAGCTCCTTGAGG314210.2769680700884974No Hit
TCAGTGCACTACAGAACTTTGC310020.27327469236763935No Hit
TAGCTTATCAGACTGATGTTG302810.26691926196969507No Hit
CCCATAAAGTAGAAAGCACT298470.2630936630893791No Hit
TGAGAACTGAATTCCATGGGTT297970.26265292589118605No Hit
CGCGACCTCAGATCAGACGT297810.26251188998776426No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC289450.2551427640339759No Hit
TGGCTCAGTTCAGCAGGAAC283700.2500742862547555No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT271180.23903822681200065No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT270140.23812149343975902No Hit
CATTGCACTTGTCTCGGTCTGA268620.23678165235725204No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT264100.2327973880855866No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT261910.23086695915750088No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT257180.22669758526259431No Hit
AAACCGTTACCATTACTGAG250530.2208357805266263No Hit
CGCGACCTCAGATC249830.22021874844915598No Hit
TAGCTTATCAGACTGATGTTGAT230980.20360295607727677No Hit
GGCTGGTCCGATGGTAGTGGGTT214810.18934951508771244No Hit
TGTAAACATCCCCGACTGGAAGC214170.18878537147402527No Hit
AGCTCGGTCTGAGGCCCCTCAGT203170.17908915311377746No Hit
AAAAGCTGGGTTGAGAGGGCGA202370.17838397359666852No Hit
TGGCTCAGTTCAGCAGGAACAG201430.17755538766406553No Hit
TGAGGTAGTAGTTTGTACAGTT201330.17746724022442692No Hit
TGAGGTAGTAGGTTGTATAG199960.17625962030137785No Hit
TATTGCACTTGTCCCGGCCTG196510.17321853363384557No Hit
TGAGGTAGTAGGTTGTATAGTC192560.16973670976812022No Hit
TTCACAGTGGCTAAGTTCCG185560.16356638899341708No Hit
TGAGGTAGTAGGTTGTGTGG179830.15851554070212434No Hit
TGAGGGGCAGAGAGCGAGACTT178930.1577222137453768No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG175130.15437261103910935No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT172960.15245981159895136No Hit
GGCTGGTCCGATGGTAGTGGGT172050.15165766989823995No Hit
TGTAAACATCCTTGACTGGAAGCT170930.15067041857428745No Hit
TTCAAGTAATTCAGGATAGGT166890.14710926201288735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA163610.1442180259927407No Hit
TGAGGTAGTAGATTGTATAGTC161120.14202315474573915No Hit
TACCCTGTAGAACCGAATTTGT157040.13842673920848358No Hit
AAACCGTTACCATTACTG150400.13257374921647944No Hit
TGAGGTAGTAGTTTGTGCTGT147540.130052732442815No Hit
TAGCTTATCAGACTGATGTTGACT146510.12914481381453724No Hit
AAGCTGCCAGTTGAAGAACT146420.12906548111886249No Hit
AAGCTGCCAGTTGAAGAACTGC145080.12788430542770504No Hit
AACCCGTAGATCCGATCTTGT143400.1264034284417763No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC143280.12629765151420994No Hit
TCAGTGCATCACAGAACTTTGT140430.12378544948450937No Hit
TTCAAGTAATCCAGGATAGGCA138390.12198724171588159No Hit
TCCTGTACTGAGCTGCCCCGAA136780.12056806793769986No Hit
CGCGACCTCAGATCAGAC134420.11848778836222851No Hit
TGAGGTAGTAGGTTGTATAGC131590.11599321582045564No Hit
TGTAAACATCCCCGACTGGAAG130890.11537618374298535No Hit
TGAGGGGCAGAGAGCGAGACTTC129520.11416856381993629No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT127740.11259953939436891No Hit
TCACAGTGAACCGGTCTCTTT127700.11256428041851348No Hit
TGAGGTAGTAGGTTGTGTGGTTT122940.1083684622917153No Hit
TGAGGTAGTAGGTTGTAT121830.10739002571172668No Hit
CTAGACTGAAGCTCCTTGAGGA121530.10712558339281081No Hit
AGAGGTAGTAGGTTGCATAGTT120960.1066231429868707No Hit
GGCTGGTCCGATGGTAGT120240.10598848142147266No Hit
GCATTGGTGGTTCAGTGGTAGAATT119680.10549485575949641No Hit
AGTTGGTCCGAGTGTTGT117870.10389938710203746No Hit
AAACCGTTACCATTACTGAGG114900.10128140814477052No Hit
TGTAAACATCCCCGACTGGAAGCC114450.10088474466639676No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)