FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13568206
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11908948.777092564779752No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5159963.802978816801573No Hit
TAGCTTATCAGACTGATGTTGAC3147632.3198571719798475No Hit
TTCAAGTAATCCAGGATAGGCT2963532.1841723216761304No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1987951.4651531676332155No Hit
TCAGTGCACTACAGAACTTTGT1843061.3583667582877206No Hit
CGCGACCTCAGATCAGA1749181.2891755918210557No Hit
TAGCTTATCAGACTGATGTTGA1698571.251875155786992No Hit
TGGAGTGTGACAATGGTGTTT1675881.2351522375176203No Hit
TCCTGTACTGAGCTGCCCCGAG1534631.1310485704594992No Hit
TAGCTTATCAGACTGATGTTG1422121.0481267751978414No Hit
TCGTACCGTGAGTAATAATGCG1368691.0087479509081745No Hit
AAACCGTTACCATTACTGAGC1360511.0027191509327025No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1205230.8882751337944015No Hit
AAGCTGCCAGTTGAAGAACTGT1187900.8755026272448988No Hit
AAACCGTTACCATTACTGAG1139300.8396835956057861No Hit
TGAGGTAGTAGTTTGTGCTGTT1111350.8190839673277366No Hit
TTCACAGTGGCTAAGTTCCG982490.7241119422862536No Hit
TCCTGTACTGAGCTGCCCCGA955310.7040798171843794No Hit
TGAGGTAGTAGGTTGTATAGT811710.598244159913256No Hit
TATTGCACTTGTCCCGGCCTGT774020.5704659849651458No Hit
TGAGGGGCAGAGAGCGAGACTTT772800.5695668240886083No Hit
TGGCTCAGTTCAGCAGGAAC767150.5654026774062835No Hit
TGAGGTAGTAGGTTGTATAGTT734570.5413906599000634No Hit
TGAGGTAGTAGGTTGTGTGGT722840.5327454491772899No Hit
TGAGGTAGTAGATTGTATAGTT706520.5207173299108224No Hit
TGAGGTAGTAGATTGTATAGT613520.45217473850264356No Hit
TGAGGTAGTAGGTTGTGTGGTT583540.43007896548740493No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT578270.4261948853076081No Hit
TGAGAACTGAATTCCATGGGTT521390.38427335198183166No Hit
CTAGACTGAAGCTCCTTGAGG518940.38246766005763766No Hit
AAAAGCTGGGTTGAGAGGGCGA501650.36972463419261176No Hit
CCCATAAAGTAGAAAGCACT497470.3666439026648033No Hit
CGCGACCTCAGATCAGACGT495580.36525094032328226No Hit
TGAGGGGCAGAGAGCGAGACT486760.358750449396184No Hit
TGAGGTAGTAGTTTGTACAGT474980.3500683878178147No Hit
TACAGTACTGTGATAACTGAAG436760.32189959380038896No Hit
TTCAAGTAATCCAGGATAGGCC425500.3136007811202159No Hit
AAACCGTTACCATTACTG422700.3115371332068514No Hit
TCCTGTACTGAGCTGCCCCG397470.2929421914732132No Hit
TGAGGTAGTAGGTTGTATAG393210.28980249857645146No Hit
CATTGCACTTGTCTCGGTCTGA389440.2870239440645285No Hit
TGAGGTAGTAGGTTGTGTGG386960.28519614162697704No Hit
TCCTGTACTGAGCTGCCCCGAGA385260.28394321253672006No Hit
TACAGTATAGATGATGTACT371660.27391977981466376No Hit
TGAGATGAAGCACTGTAGCTC337150.24848531928244605No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC331820.2445570180759343No Hit
TGTAAACATCCCCGACTGGAAGCT322510.23769538876399726No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC304030.2240753125357914No Hit
TACAGTACTGTGATAACTGA299190.22050814971411845No Hit
TGTAAACATCCCCGACTGGAAG282920.20851688130324675No Hit
TGGCTCAGTTCAGCAGGAACAG282620.20829577616967193No Hit
AAAAGCTGGGTTGAGAGGGCG273290.2014194065154966No Hit
AGGAAACTCTGGTG266680.19654772340573248No Hit
TACAGTACTGTGATAACTGAA263580.1942629703587932No Hit
TGTAAACATCCCCGACTGGAAGC260850.19225091364326277No Hit
TGAGGTAGTAGATTGTATAG248470.18312664179774393No Hit
CAACGGAATCCCAAAAGCAGCTG247800.18263284033276028No Hit
CATTGCACTTGTCTCGGTCTG246970.18202111612987007No Hit
TTCAAGTAATTCAGGATAGGT240720.1774147591803957No Hit
TGGAGTGTGACAATGGTGTTTG239320.17638293522371343No Hit
CGCGACCTCAGATC238260.17560169708508258No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC238010.1754174428071036No Hit
TGAGGTAGTAGTTTGTGCTGT231530.17064157192188856No Hit
TGTAAACATCCTTGACTGGAAGCT229850.16940338317386985No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT227570.1677229841587016No Hit
TCCTGTACTGAGCTGCCCCGAGT227060.16734710543162448No Hit
TCAGTGCACTACAGAACTTTGC227010.1673102545760287No Hit
CGCGACCTCAGATCAGAC224950.16579199932548194No Hit
TCGTACCGTGAGTAATAATGC222550.1640231582568838No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT213990.15771429177888366No Hit
AACATTCAACGCTGTCGGTGA212530.15663824679548646No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA212060.15629184875288596No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA210030.1547957040156967No Hit
TCGTACCGTGAGTAATAATG205460.15142753581424104No Hit
TGGAGAGAAAGGCAGTTCCTG190170.1401585441730469No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA189290.13950996911456093No Hit
TCAGTGCATCACAGAACTTTGT186560.1374979123990305No Hit
TATTGCACTTGTCCCGGCCTG181090.1334664287968505No Hit
AACCCGTAGATCCGATCTTGT179720.13245671535352574No Hit
TGAGGTAGTAGTTTGTACAGTT176230.12988452563293926No Hit
AAGCTGCCAGTTGAAGAACT174700.1287568894517079No Hit
TGAGGTAGTAGTTTGTGCTG172260.12695856769863312No Hit
TGAGATGAAGCACTGTAGCT171580.12645739606253031No Hit
TAGCTTATCAGACTGATGTTGACA169830.12516761611667748No Hit
CTAGACTGAAGCTCCTTGAGGA168940.12451167088707232No Hit
TACAGTATAGATGATG168150.12392942736865876No Hit
TAGCTTATCAGACTGATGTTGACT165460.12194685133760498No Hit
TGGAGAGAAAGGCAGTTCCTGA163640.12060548019391805No Hit
AGCTCGGTCTGAGGCCCCTCAGT161840.11927884939246942No Hit
AAACCGTTACCATTACTGAGTT159480.11753948900834789No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT158690.11695724548993433No Hit
TTCACAGTGGCTAAGTTCCGA155280.11444401713830112No Hit
AAGCTGCCAGTTGAAGAACTGC153320.11299946359894594No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG150520.11093581568558143No Hit
GTACAGTACTGTGATAACTGA149220.10997769344009076No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT147370.10861421178304632No Hit
TGAGAACTGAATTCCATGGGT145190.10700751447906967No Hit
TACCCTGTAGAACCGAATTTGT140520.10356564456642241No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)