FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11286766
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT154310013.671763904735865No Hit
TTCAAGTAATCCAGGATAGGCT3156172.796345738008567No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2736532.42454747444928No Hit
TAGCTTATCAGACTGATGTTGAC2221971.9686507189038915No Hit
AAACCGTTACCATTACTGAGC1924231.7048550488244374No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1690031.4973553983488272No Hit
TCAGTGCACTACAGAACTTTGT1547271.3708709828838481No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1356821.2021335429475546No Hit
TCGTACCGTGAGTAATAATGCG1331351.1795672914632942No Hit
TCCTGTACTGAGCTGCCCCGAG1282381.1361801954607724No Hit
TAGCTTATCAGACTGATGTTGA1200081.0632629399776694No Hit
AAGCTGCCAGTTGAAGAACTGT1177711.043443267983052No Hit
AAACCGTTACCATTACTGAG1048070.9285830857129491No Hit
TGGAGTGTGACAATGGTGTTT1032940.9151780058167238No Hit
TAGCTTATCAGACTGATGTTG947140.8391597734904754No Hit
TGAGGTAGTAGGTTGTGTGGT878340.7782034286880759No Hit
TGAGGTAGTAGTTTGTGCTGTT796890.7060392675811654No Hit
TCCTGTACTGAGCTGCCCCGA765860.6785468928832227No Hit
TATTGCACTTGTCCCGGCCTGT694450.6152781053492204No Hit
TTCACAGTGGCTAAGTTCCG645470.571882149412861No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC628020.5564215648663222No Hit
TGAGGGGCAGAGAGCGAGACTTT601470.5328984405276055No Hit
TGAGGTAGTAGGTTGTATAGT596370.528379874270451No Hit
TGAGGTAGTAGGTTGTGTGGTT572760.5074615704799763No Hit
TGAGGTAGTAGATTGTATAGTT532190.4715168189010032No Hit
TGAGGGGCAGAGAGCGAGACT525000.46514652647179894No Hit
TTCAAGTAATCCAGGATAGGCC504180.446700144222003No Hit
TGAGGTAGTAGGTTGTATAGTT493630.4373529140233793No Hit
CTAGACTGAAGCTCCTTGAGG467400.41411330756746445No Hit
CCCATAAAGTAGAAAGCACT464380.41143760754852193No Hit
TGGCTCAGTTCAGCAGGAAC462020.40934666316285817No Hit
TGAGGTAGTAGATTGTATAGT455150.4032598886164558No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT433370.38396295271825426No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC408030.3615118803738821No Hit
TGAGGTAGTAGTTTGTACAGT392850.34806250080846896No Hit
TGTAAACATCCCCGACTGGAAGCT388600.3442970289275068No Hit
TGAGGTAGTAGGTTGTGTGG375140.33237155798215356No Hit
TCCTGTACTGAGCTGCCCCGAGA359630.31862980060010104No Hit
TGAGAACTGAATTCCATGGGTT353460.3131632214223277No Hit
CGCGACCTCAGATCAGA351080.3110545571689889No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT331220.2934587285675986No Hit
TACAGTACTGTGATAACTGAAG326690.2894451785391848No Hit
AGGAAACTCTGGTG322660.28587462520264884No Hit
AAACCGTTACCATTACTG304120.26944830786781615No Hit
CATTGCACTTGTCTCGGTCTGA292580.25922394421927414No Hit
TCCTGTACTGAGCTGCCCCG285680.25311058987135904No Hit
TGGCTCAGTTCAGCAGGAACAG273300.2421419917804622No Hit
TACAGTATAGATGATGTACT272950.24183189409614764No Hit
TGAGATGAAGCACTGTAGCTC271850.24085730137401623No Hit
CGCGACCTCAGATCAGACGT263030.23304283972929002No Hit
TTCAAGTAATTCAGGATAGGT256880.22759398041919182No Hit
TGTAAACATCCCCGACTGGAAGC247660.21942512142096326No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC242470.21482681575927062No Hit
TGAGGTAGTAGGTTGTATAG237180.2101399107592024No Hit
TCCTGTACTGAGCTGCCCCGAGT229330.20318486269671931No Hit
TGTAAACATCCTTGACTGGAAGCT221440.196194374898886No Hit
TCAGTGCACTACAGAACTTTGC211550.18743190033354107No Hit
TGGAGTGTGACAATGGTGTTTG210660.1866433662219984No Hit
AAAAGCTGGGTTGAGAGGGCGA208790.18498655859437504No Hit
TGTAAACATCCCCGACTGGAAG199500.1767556800592836No Hit
AACATTCAACGCTGTCGGTGA199380.1766493608532329No Hit
TGAGGTAGTAGTTTGTGCTGT190000.16833874291360343No Hit
AAGCTGCCAGTTGAAGAACT189260.16768310780962412No Hit
TCAGTGCATCACAGAACTTTGT184690.1636341180458601No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC184660.1636075382443474No Hit
CTAGACTGAAGCTCCTTGAGGA181780.16105587729913068No Hit
TACAGTACTGTGATAACTGAA181310.16063946040876545No Hit
TACAGTACTGTGATAACTGA177340.15712206667525488No Hit
TCGTACCGTGAGTAATAATGCA176530.1564044120344127No Hit
CATTGCACTTGTCTCGGTCTG174640.1547298845391142No Hit
AGCTCGGTCTGAGGCCCCTCAGT173570.1537818716184955No Hit
TCGTACCGTGAGTAATAATGC170240.15083151365058867No Hit
TGGAGAGAAAGGCAGTTCCTG167800.14866968979422449No Hit
CAACGGAATCCCAAAAGCAGCTG166380.14741157918929124No Hit
TATTGCACTTGTCCCGGCCTG164650.1458788106353937No Hit
AAGCTGCCAGTTGAAGAACTGC163530.14488649804558718No Hit
AAACCGTTACTATTACTGAGT151710.13441405624959354No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT151510.1342368575728424No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT151370.13411281849911658No Hit
TACAGTATAGATGATG151170.1339356198223654No Hit
AAACCGTTACCATTACTGAGTT146120.12946135323439859No Hit
AAAAGCTGGGTTGAGAGGGCG145760.12914239561624652No Hit
TGAGGTAGTAGTTTGTGCT144350.1278931449451508No Hit
TGAGGTAGTAGATTGTATAG141330.12521744492620826No Hit
TAGCTTATCAGACTGATGTTGACT140050.12408337339500083No Hit
CGTACCGTGAGTAATAATGCG138180.12242656576737748No Hit
TACCCTGTAGAACCGAATTTGT138050.12231138662748922No Hit
TGGCTCAGTTCAGCAGGAACA137610.12192154953863668No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG131250.11628663161794973No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT131050.11610943294119856No Hit
ACCAATATTACTGTGCTGCTT130580.11569301605083335No Hit
TAGCTTATCAGACTGATGTTGAT129890.11508168061604183No Hit
AACCCGTAGATCCGATCTTGT128790.11410708789391043No Hit
TTCACAGTGGCTAAGTTCCGA127480.11294643656119033No Hit
TGAGGTAGTAGTTTGTGCTG126570.11214018258197254No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA126180.11179464516230778No Hit
TTCAAGTAATCCAGGATAGGCA125880.11152884714718105No Hit
TCGTACCGTGAGTAATAATG125360.11106813058762803No Hit
TGAGATGAAGCACTGTAGCT123580.10949106236454269No Hit
AGACCGTTACCATTACTGAGT122750.10875568785602535No Hit
TAGCAGCACGTAAATATTGGC119600.10596480869719457No Hit
TGAGGTAGTAGTTTGTACAGTT117530.10413080239282005No Hit
TGAGGTAGTAGGTTGTGTGGC117410.10402448318676935No Hit
TAGCTTATCAGACTGATGTTGACA117280.1039093040468811No Hit
TCACAGTGAACCGGTCTCTTT116550.10326252887673935No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT116160.10291699145707459No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA113270.10035647057802032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)