FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11342269
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11331369.990381994995886No Hit
AAACCGTTACCATTACTGAGC2639052.3267390325516No Hit
TTCAAGTAATCCAGGATAGGCT2523812.2251367870044345No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2164931.9087274336378375No Hit
TCAGTGCACTACAGAACTTTGT2029501.7893245169903835No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1838651.6210601247422363No Hit
TGGAGTGTGACAATGGTGTTT1496791.3196565872313555No Hit
TAGCTTATCAGACTGATGTTGAC1375051.2123235659461082No Hit
CGCGACCTCAGATCAGA1243271.096138700290039No Hit
TGAGGTAGTAGTTTGTGCTGTT1117400.9851644322665949No Hit
TGAGGTAGTAGGTTGTATAGT1058810.9335081014213295No Hit
AAGCTGCCAGTTGAAGAACTGT975410.8599778404127076No Hit
TGAGGTAGTAGGTTGTGTGGT884990.7802583416069572No Hit
TAGCTTATCAGACTGATGTTGA878710.774721530586164No Hit
TCCTGTACTGAGCTGCCCCGAG838700.7394464017737544No Hit
TATTGCACTTGTCCCGGCCTGT820860.7237176265172338No Hit
TGAGGTAGTAGATTGTATAGT815360.7188685085850107No Hit
TGAGGGGCAGAGAGCGAGACTTT726000.6400835670534706No Hit
TGAGGTAGTAGGTTGTATAGTT717170.6322985286277376No Hit
TGAGGTAGTAGATTGTATAGTT714520.6299621354422118No Hit
TTCAAGTAATCCAGGATAGGCC697950.6153530655991318No Hit
TAGCTTATCAGACTGATGTTG659320.5812946245588074No Hit
TGAGGTAGTAGGTTGTGTGGTT630540.5559205129061918No Hit
CCCATAAAGTAGAAAGCACT596420.5258383485702905No Hit
TCCTGTACTGAGCTGCCCCGA565940.49896541864771504No Hit
AAACCGTTACCATTACTGAG557060.4911362973316891No Hit
TGAGGTAGTAGTTTGTACAGT551040.4858287173404193No Hit
CGCGACCTCAGATCAGACGT529450.4667937253119283No Hit
TCAGTGCACTACAGAACTTTGC503270.4437119239545456No Hit
TGAGGGGCAGAGAGCGAGACT503260.443703107376487No Hit
TCGTACCGTGAGTAATAATGCG498980.4399296119674115No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC492690.43438398436855974No Hit
TTCACAGTGGCTAAGTTCCG458830.4045310510621816No Hit
TACAGTATAGATGATGTACT418500.36897379175189726No Hit
TGAGAACTGAATTCCATGGGTT418310.3688062767687841No Hit
TAGCTTATCAGACTGATGTTGAT414920.36581745680692285No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT394140.3474966076011775No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT369550.32581664215511025No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC362360.3194775225309856No Hit
TGGCTCAGTTCAGCAGGAAC357200.3149281682527543No Hit
TGGAGTGTGACAATGGTGTTC350600.3091092267340864No Hit
TGAGATGAAGCACTGTAGCT304170.26817385480806355No Hit
TGAGGTAGTAGTTTGTGCTGTC288540.25439354330249087No Hit
TTCAAGTAATCCAGGATAGGCA285750.2519337180241449No Hit
TGTAAACATCCCCGACTGGAAGCT284020.2504084500200092No Hit
AAACCGTTACCATTACTGAGG278680.2457003973367234No Hit
AAACCGTTACCATTACTGAGA274320.2418563693031791No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT252270.22241581468399313No Hit
TTCAAGTAATTCAGGATAGGT251140.22141954136337272No Hit
CATTGCACTTGTCTCGGTCTGA249320.21981492415670975No Hit
TGAGGTAGTAGGTTGTATAGC248250.21887155030444086No Hit
AAGCTGCCAGTTGAAGAACTGC247690.21837782193315994No Hit
AAAAGCTGGGTTGAGAGGGCGA242250.21358160346928817No Hit
TCCTGTACTGAGCTGCCCCGAGA237310.20922621390834584No Hit
CTAGACTGAAGCTCCTTGAGG236120.2081770411193739No Hit
TATTGCACTTGTCCCGGCCTGC234740.2069603533472888No Hit
TCCTGTACTGAGCTGCCCCGAGT234600.2068369212544686No Hit
TGAGGTAGTAGGTTGTGTGGC232400.2048972740815793No Hit
TGAGGTAGTAGTTTGTGCTGT231760.20433301308582963No Hit
AAACCGTTACCATTACTG228510.20146762521678863No Hit
TGAGGTAGTAGGTTGTATAG225380.19870803628445066No Hit
TGAGGTAGTAGGTTGTGTGG220670.19455542801885584No Hit
TCCTGTACTGAGCTGCCCCG220560.1944584456602114No Hit
TGTAAACATCCTTGACTGGAAGCT210070.18520985527675282No Hit
TACAGTACTGTGATAACTGAAG204430.18023730525170933No Hit
TGAGATGAAGCACTGTAGCTC191280.16864350510466644No Hit
TGAGGTAGTAGATTGTATAGC190020.1675326162692844No Hit
TGAGGTAGTAGTTTGTACAGTT187460.16527557228628592No Hit
TGAGGTAGTAGGTTGTATAGTC186650.16456142946354033No Hit
TGAGGTAGTAGATTGTATAGTC184640.16278929727376418No Hit
AAACCGTTACCATTACTGAGTT184580.16273639780541266No Hit
TGAGGTAGTAGGTTGTGTGGTC174060.15346135768777833No Hit
AAGCTGCCAGTTGAAGAACT173330.15281774748950144No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT171040.15079875111408486No Hit
AACCCGTAGATCCGATCTTGT169020.14901780234625012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT167520.14769531563746197No Hit
CGCGACCTCAGATC167210.14742200171764575No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA161800.14265223298794977No Hit
TCAGTGCATCACAGAACTTTGT160380.1414002789036303No Hit
TGAGGTAGTAGATTGTATAG158600.1398309280092017No Hit
TGAGGTAGTAGATTGTAT156600.13806761239748414No Hit
TACAGTACTGTGATAACTGA155110.13675394226675455No Hit
CCCATAAAGTAGAAAGCACC154550.13626021389547366No Hit
TGAGGGGCAGAGAGCGAGACTT154270.13601334970983317No Hit
TCGTACCGTGAGTAATAATGCA151430.13350944154119426No Hit
TAGCTTATCAGACTGATGTTGACT150370.13257488426698397No Hit
TGAGGGGCAGAGAGCGAGACTTC149830.13209878905182024No Hit
AGCTCGGTCTGAGGCCCCTCAGT149310.13164032699277367No Hit
TACAGTACTGTGATAACTGAA148320.1307674857649735No Hit
TGAGGTAGTAGGTTGTAT148160.1306264205160361No Hit
CAACGGAATCCCAAAAGCAGCT145210.1280255299887527No Hit
TGAGAACTGAATTCCATGGGT144880.1277345829128193No Hit
CGCGACCTCAGATCAGACGC138110.1217657595671554No Hit
TGAGGGGCAGAGAGCGAGACC134210.11832729412430618No Hit
TGAGGTAGTAGTTTGTACAGC133720.11789528179943537No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC132300.11664332771511592No Hit
TAGCTTATCAGACTGATGT130470.11502989393039435No Hit
TACCCTGTAGAACCGAATTTGT127320.11225267184193921No Hit
TCCTGTACTGAGCTGCCCCGAA126970.11194409160988864No Hit
AAAAGCTGGGTTGAGAGGGCGT125200.1103835572935186No Hit
AAAAGCTGGGTTGAGAGGGCG122640.10812651331052014No Hit
TGTAAACATCCCCGACTGGAAG121940.10750935284641899No Hit
TTCACAGTGGCTAAGTTCCA121770.107359471019423No Hit
CATTGCACTTGTCTCGGTCTG121460.1070861570996068No Hit
TGGAGTGTGACAATGGTGTTTG121030.10670704424308752No Hit
TGAGGTAGTAGTTTGTGCT116280.10251916966525834No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT114670.10109970059782569No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC113610.10016514332361541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)