FastQCFastQC Report
Wed 8 May 2024
EGAF00002167728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13563662
Total Bases300.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT178661213.172047489829811No Hit
TCCTGTACTGAGCTGCCCCGA3265012.407174404670361No Hit
AAACCGTTACCATTACTGAGC3235612.3854988424217587No Hit
TCAGTGCACTACAGAACTTTGT2734022.015694581596032No Hit
CGCGACCTCAGATCAGA2678481.9747469378107476No Hit
TCCTGTACTGAGCTGCCCCGAG2238481.6503507681037761No Hit
TATTGCACTTGTCCCGGCCTGT2111091.5564307043333871No Hit
TGAGGTAGTAGGTTGTGTGGT1978391.458595768605853No Hit
TGAGGTAGTAGGTTGTATAGT1962841.4471313130627999No Hit
TGAGGTAGTAGGTTGTGTGGTT1927731.4212459732482274No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1754291.2933749012619158No Hit
TTCAAGTAATCCAGGATAGGCT1647671.21476781122974No Hit
TGGAGTGTGACAATGGTGTTT1603791.1824166659416904No Hit
TGAGGGGCAGAGAGCGAGACTTT1538741.134457641306603No Hit
TGAGGTAGTAGGTTGTATAGTT1496701.1034630618191459No Hit
TGAGGGGCAGAGAGCGAGACT1493431.1010522084670054No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1383891.0202923074904109No Hit
TCCTGTACTGAGCTGCCCCGAGT1236450.9115901000776928No Hit
AAGCTGCCAGTTGAAGAACTGT1146300.8451257484888668No Hit
TGAGGTAGTAGATTGTATAGT1079420.7958175306934071No Hit
TCCTGTACTGAGCTGCCCCGAGA1047420.7722250819874455No Hit
TGAGGTAGTAGATTGTATAGTT978510.7214202182272015No Hit
TGAGGTAGTAGTTTGTGCTGTT834720.6154090244950073No Hit
TCCTGTACTGAGCTGCCCCG808090.595775683587515No Hit
TAGCTTATCAGACTGATGTTGAC703990.5190264988909338No Hit
TGAGGTAGTAGTTTGTACAGT675750.49820616290792263No Hit
TGAGGTAGTAGGTTGTGTGG574450.4235213174731131No Hit
TCGTACCGTGAGTAATAATGCG572730.42225322335516763No Hit
CGCGACCTCAGATCAGACGT540550.39852806712523503No Hit
TCAGTGCACTACAGAACTTTGC517570.3815857398982664No Hit
TATTGCACTTGTCCCGGCCTGC487580.35947519187664806No Hit
CATTGCACTTGTCTCGGTCTGA484470.3571823007680374No Hit
TGTAAACATCCCCGACTGGAAGCT473090.34879223619697985No Hit
CCCATAAAGTAGAAAGCACT418170.30830169610537334No Hit
TGAGGTAGTAGGTTGTGTGGTC412230.3039223478143292No Hit
TAGCTTATCAGACTGATGTTGA405790.29917436751225446No Hit
TGAGGTAGTAGGTTGTGTGGC400800.29549542004216856No Hit
AAGCTGCCAGTTGAAGAACT386510.2849599171669126No Hit
TTCAAGTAATCCAGGATAGGCC363480.2679807267388409No Hit
TGAGGTAGTAGGTTGTATAGC358730.2644787226340497No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT354730.2615296665458045No Hit
AAAAGCTGGGTTGAGAGGGCGA331050.24407125450339298No Hit
TGAGGGGCAGAGAGCGAGACTT330530.2436878772119211No Hit
AAACCGTTACCATTACTG327330.24132863234132496No Hit
TGAGGTAGTAGGTTGTATAG326470.2406945852823522No Hit
TGAGGGGCAGAGAGCGAGACC307300.22656123397943712No Hit
AAACCGTTACCATTACTGAGA303210.2235458241292064No Hit
AAACCGTTACCATTACTGAG296450.21856191934007202No Hit
TGAGGTAGTAGGTTGTATAGTC287940.21228780251233034No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG286950.2115579111304897No Hit
TCCTGTACTGAGCTGCCCCGAA286410.2111597885585766No Hit
TTCAAGTAATTCAGGATAGGT281600.20761354861246173No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC277260.2044138227567157No Hit
TGGAGTGTGACAATGGTGTTC275650.20322682768119704No Hit
TAGCTTATCAGACTGATGTTG274980.20273286078641592No Hit
TATTGCACTTGTCCCGGCCTG269530.19871477186618186No Hit
CTAGACTGAAGCTCCTTGAGG265880.19602375818565812No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT253360.18679321262945067No Hit
TGAGGTAGTAGTTTGTACAGTT248450.1831732462811297No Hit
TGAGGTAGTAGGTTGTAT244570.18031266187553185No Hit
TCCTGTACTGAGCTGCCCCGAGC241040.17771011987765545No Hit
TGAGGGGCAGAGAGCGAGACTTC233390.1720700501088865No Hit
TGGCTCAGTTCAGCAGGAAC227000.16735893300791482No Hit
TGAGGTAGTAGTTTGTGCTGT226420.16693131987511928No Hit
TCCTGTACTGAGCTGCCCCA223910.16508078717974542No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC222730.16421081563371306No Hit
AAGCTGCCAGTTGAAGAACTGC221920.16361363177584343No Hit
CATTGCACTTGTCTCGGTCTG214190.1579145808853096No Hit
CGCGACCTCAGATC210190.15496552479706438No Hit
ACCAATATTACTGTGCTGCTT205970.15185427062396573No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT205360.1514045395705083No Hit
AGCTCGGTCTGAGGCCCCTCAGT201780.14876513437152886No Hit
CGCGACCTCAGATCAGAC194970.14374436638129143No Hit
TGTAAACATCCTTGACTGGAAGCT194140.14313243724298055No Hit
TGAGGTAGTAGATTGTATAGC193090.1423583100198162No Hit
TGAGGTAGTAGATTGTAT189400.13963780577841No Hit
TGAGGTAGTAGATTGTATAGTC186010.13713848074362217No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT178710.13175645338257472No Hit
TGAGGTAGTAGGTTGTGTGGTTT172670.12730337868932445No Hit
TGTAAACATCCCCGACTGGAAGC167760.12368341234100348No Hit
TATTGCACTTGTCCCGGCCTGTA164390.1211988325866569No Hit
TGAGGTAGTAGTTTGTGCTGTC163500.12054266760702236No Hit
TGTAAACATCCCCGACTGGAAG161930.11938516309238613No Hit
AAACCGTTACCATTACTGAGG161920.11937779045216551No Hit
AAAAGCTGGGTTGAGAGGGCGT158850.11711438990443732No Hit
TGAGGTAGTAGGTTGTGT152730.11260233408942216No Hit
GGCTGGTCCGATGGTAGTGGGTT149160.10997030153066331No Hit
TACAGTACTGTGATAACTGAAG148820.10971963176316249No Hit
TCGTACCGTGAGTAATAATGCA147870.10901923094220425No Hit
AAATGGATTTTTGGAGCAGGGAGA147270.10857687252896747No Hit
TGAGAACTGAATTCCATGGGTT147100.10845153764521705No Hit
TACAGTATAGATGATGTACT146190.10778062738514127No Hit
TGAGGTAGTAGTTTGTGCT146040.10767003778183208No Hit
TTCAAGTAATCCAGGATAGGCA145410.10720556144793346No Hit
AAACCGTTACTATTACTGAGT143690.10593746732998802No Hit
TAGCTTATCAGACTGATGTTGAT143580.10585636828756129No Hit
TGAGGTAGTAGATTGTATAG143360.1056941702027078No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT141280.10416066103682028No Hit
CAACGGAATCCCAAAAGCAGCT140850.10384363750733394No Hit
TTCACAGTGGCTAAGTTCCG137920.10168345392269433No Hit
AACCCGTAGATCCGATCTTGT136560.10068077485269097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)