FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002168659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39127771
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG3002400.7673322357156507No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2453070.6269383451462134No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG1781140.45521121047248003No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA1500100.3833849876089287No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC1342060.3429942380310905No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG1328070.3394187724110325No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1128830.28849841714724817No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA1099800.2810791343059128No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA940120.24026924508426506No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC847380.2165674093727445No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA814850.20825362119401078No Hit
TCCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTC757080.1934891716678673No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA698950.1786327158784486No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG687410.1756834039945695No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC685620.1752259284077286No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA678310.1733576901173338No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCC676110.17279542961954056No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC674950.1724989649934314No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC650100.16614797709790316No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA595180.1521119104893555No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATT555840.14205767049699813No Hit
CTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA533550.13636094936253845No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG528690.13511886480832247No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC523900.13389467036085445No Hit
CCAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGT515200.13167118566503572No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG502420.12840496331876405No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA496440.12687663705658062No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA495600.126661955775605No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT495420.12661595264396738No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT489780.12517452118598835No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA484610.1238532090161742No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG480960.12292036773574452No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC473240.12094734453439733No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA473140.12092178723904308No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCC468200.11965925684854371No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA466620.11925545158194674No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC465050.11885420204488521No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTG445530.1138654179917379No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG440830.11266422511008868No Hit
CGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCA439690.11237287194305037No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG430850.1101136070337357No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG429950.10988359137554757No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA421940.10783645201767307No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC418320.10691127792584963No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAAC414560.10595032362053029No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTG412390.1053957303113433No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCAT410090.10480791251819582No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC408780.10447311194905533No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG400330.10231352049162216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)