FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002168680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186989486
Sequences flagged as poor quality0
Sequence length20-95
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCT40890052.1867566393545785No Hit
TTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC40073682.1430980349344346No Hit
ATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGA30256051.6180615630977242No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGC26111641.3964228983441347No Hit
TAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC23963601.2815479903506446No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC23461221.2546812391366218No Hit
AGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTT18261690.9766158724025799No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA15828690.8465016049084171No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT15197060.8127227003554628No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG13685940.7319096005216037No Hit
GGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGC11063590.5916690952345846No Hit
ATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAGAAGAC10826350.5789817508776938No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT10766980.5758067060519113No Hit
CCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC10154300.5430412274623825No Hit
ATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAG8324330.4451763667610702No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC7788400.4165153970207715No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG7481860.4001219619374749No Hit
CCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGG7364720.39385743859416783No Hit
ACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGC7100840.37974541520478855No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA6995500.374111943384881No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG6706000.35862978948452745No Hit
TTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCC6620980.35408300977949103No Hit
CTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGC6464750.3457279945675662No Hit
TCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGAC6443660.34460012366684617No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA6328310.3384313276309022No Hit
TCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCG6021070.3220004572877429No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAAC5850660.3128871106688854No Hit
TGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTT5728330.30634503161316784No Hit
TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGA5601580.2995665756308887No Hit
CTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGC5370060.2871851308260187No Hit
TTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT5241750.28032324769318845No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT4924450.2633543791868598No Hit
TGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCG4760520.2545875761164454No Hit
AGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACC4675850.25005951404134025No Hit
TTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCAC4664620.24945894551525746No Hit
TTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTAT4570690.2444356684311117No Hit
GCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGG4529300.24222217499437373No Hit
CGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGA4476400.23939313892760794No Hit
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG4421000.2364304055041897No Hit
GATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTA4388010.23466613518580395No Hit
AGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGA4373090.2338682293613022No Hit
AACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA4297410.22982094298071923No Hit
TGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCC4240830.22679510440496106No Hit
TGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAG3984330.21307775561241982No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG3959390.21174399078245498No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATG3951660.21133059855568564No Hit
TTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCG3846310.20569659194635145No Hit
GTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCA3825780.20459866925352155No Hit
TATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAA3767430.20147817294925344No Hit
GCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG3722920.1990978252114132No Hit
TACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGG3550080.18985452476188958No Hit
GAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGC3475800.18588210890103202No Hit
TCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCA3461970.18514249512403066No Hit
CTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGG3449460.1844734735513418No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA3188410.17051279556969315No Hit
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT3143340.16810249962396281No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC2964290.15852709494051448No Hit
CGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCG2941570.15731205336325702No Hit
GGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAG2794940.14947043600087762No Hit
CATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGT2784590.14891692894433647No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCC2777890.14855862002850792No Hit
GGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGG2754850.14732646518959894No Hit
TATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC2647770.14159994000946127No Hit
TTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCA2645140.1414592903902629No Hit
TTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCG2623210.14028649717770764No Hit
ATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGGATTA2610370.13959982755394065No Hit
AGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTCAGTCGGTC2601160.13910728649203305No Hit
TAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCC2542710.1359814422934988No Hit
AACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCAT2532080.13541296113301257No Hit
GTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGC2507570.13410219224839198No Hit
GTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTA2497720.1335754246631813No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTC2463460.13174323608761618No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG2433130.13012121975670868No Hit
ATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAA2317830.12395509766789775No Hit
CAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACG2276500.12174481296771948No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC2240630.11982652329447015No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG2238270.11970031298979024No Hit
TTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAAC2206880.11802160897966209No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC2199360.11761944733085154No Hit
AGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGCCCT2142820.11459574791279976No Hit
ATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGA2139230.11440375850864686No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2137370.11430428767529742No Hit
CTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAA2129930.11390640434189973No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC2120350.11339407607120755No Hit
AAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTA2100970.11235765416243777No Hit
ATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTAC2047910.1095200614648462No Hit
TGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATT2045900.10941256879009764No Hit
AAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGC2036300.10889917094055225No Hit
ATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCC2003230.10713062230675366No Hit
CATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGG2001260.10702526878971153No Hit
GTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACAT1960570.10484921061283627No Hit
TCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTC1938690.10367909134741404No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC1930410.10323628570218113No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA1911070.1022020029511178No Hit
TCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCG1910690.10218168095290663No Hit
AAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACT1908140.10204530964912112No Hit
CTCGATGCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGA1886350.10088000348853839No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)