FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002168681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186989486
Sequences flagged as poor quality0
Sequence length20-95
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTC15043380.8045040564473236No Hit
ACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGG8883750.47509355686447524No Hit
CGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATA7084560.37887477801826785No Hit
TACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGC6282200.3359654135848044No Hit
GGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG5376540.2875316743744619No Hit
CGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGA5237080.28007350103096174No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC5085510.2719676976918371No Hit
ATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGC4994470.2670989747519815No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG4876580.2607943422016787No Hit
CGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCG4759740.2545458625411698No Hit
GCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGAC4686790.2506445736740514No Hit
GGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGAC4382830.2343891142628201No Hit
ACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTA4347780.23251467732255277No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC4328760.23149750783314094No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG4288250.22933107586594467No Hit
ACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTC4269280.2283165803236659No Hit
CCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACCCA4252470.22741759929753486No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT4115030.22006745341820985No Hit
ACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGA4045690.21635922353409753No Hit
CCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGG3894120.20825342019497287No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC3790060.20268840142167138No Hit
GCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAA3755070.20081717321796372No Hit
TTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGG3709110.19835928101326508No Hit
GCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGA3612420.193188402047375No Hit
GGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCT3457880.18492376624854726No Hit
CCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACT3455170.184778838313936No Hit
AAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAA3452170.18461840148595304No Hit
CCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACCCAT3441860.18406703358711837No Hit
GTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAG3416420.18270652928582307No Hit
GCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTG3404110.1820482035016664No Hit
TCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCC3371700.18031495097002406No Hit
AACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGT3370720.1802625416062163No Hit
ACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTG3281120.17547082834379255No Hit
AATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTT3250010.17380709843760947No Hit
TTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGT3139730.16790944064095667No Hit
ACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCC3085560.16501248631701143No Hit
CTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAA3053230.16328351210078196No Hit
GTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCCGGATTCG3020670.16154223772774048No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC2960350.1583163879064302No Hit
CGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACC2928650.15662110542407717No Hit
ATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATC2928330.15660399216242565No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCC2923610.15635157155306584No Hit
ATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTC2877220.153870683403023No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG2809620.1502555068791408No Hit
ATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGC2740440.14655583362585423No Hit
GCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTC2725810.14577343669472412No Hit
GAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTC2676700.14314708582064342No Hit
CGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCT2638920.1411266513669116No Hit
TCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGAC2624850.14037420264367162No Hit
GGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAA2609430.13954955734783933No Hit
CTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAG2560260.13691999773719898No Hit
GGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATC2509550.13420808055486072No Hit
TTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCT2390050.12781734690687369No Hit
GTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTT2346720.12550010432137343No Hit
ATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCA2340150.1251487476680908No Hit
AGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTT2339400.12510863846109507No Hit
ACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCC2303040.12316414410594187No Hit
ACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAG2255270.12060945501502689No Hit
CCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATT2208060.11808471413200207No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC2205960.11797240835241399No Hit
CCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCT2202970.11781250631385767No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG2200570.11768415685147132No Hit
TTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCT2177850.11646911527421386No Hit
CGGCGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTT2160990.11556746030094976No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC2126180.11370585830692105No Hit
GCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATG2109720.11282559491072135No Hit
CCCTTCGGAACGGCGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTC2077130.11108271616940002No Hit
ATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATC2075560.11099875422942229No Hit
TCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTG1996030.10674557391959462No Hit
ATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTC1991310.10649315331023478No Hit
CCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGAC1980420.1059107676246567No Hit
ACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCG1963500.10500590391483294No Hit
ATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTG1946450.10409408794246325No Hit
GCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAG1937060.10359192067087666No Hit
GGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGA1934830.10347266262874266No Hit
TTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGG1917520.10254694213128113No Hit
AACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTT1900890.10165758731482902No Hit
CTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTT1897910.10149822006569932No Hit
GGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAG1894870.10133564408000993No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA1892250.10119552925023816No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)