FastQCFastQC Report
Mon 23 Jan 2023
EGAF00002168746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196511307
Sequences flagged as poor quality0
Sequence length20-95
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC10230970.5206300928017338No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGC7382650.37568576143051147No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC5428590.2762482262661863No Hit
TAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCT5260580.267698591002705No Hit
ATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGA4960720.25243941815521076No Hit
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG4409380.22438301730902435No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG3698670.18821665055639777No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG3519150.17908129836009895No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG3281660.1669959886837453No Hit
CGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGA3216070.16365826725685562No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT3188000.1622298507230426No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2991190.1522146509360909No Hit
TAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC2980170.15165386895523522No Hit
AGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGA2944610.1498443038700058No Hit
CCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC2905450.1478515432193426No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG2690140.1368949217766894No Hit
AACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCG2631080.13388949675043382No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA2619170.13328342475479033No Hit
AGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTT2463930.12538362487202837No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2420910.12319443786509446No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG2381970.1212128724989855No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA2288320.11644724341485348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)