FastQCFastQC Report
Tue 14 Feb 2023
EGAF00002168874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115117046
Sequences flagged as poor quality0
Sequence length20-95
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC11889011.0327758062867596No Hit
TAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCT11261060.9782269777839852No Hit
ATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGA9672290.8402135336238561No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGC8345250.7249360794056512No Hit
TAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC6261090.5438890431569969No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG4738110.41159065183100685No Hit
AGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTT4242800.3685640091911323No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT3662210.31812925428958627No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC3511790.30506255346406297No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA3083700.26787518505295904No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG3076720.26726884565818343No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT2230710.19377755749569878No Hit
CCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC2093850.18188878821647317No Hit
ACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGC2063440.17924712904811682No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAAC1977340.17176778493777542No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA1780950.15470775718133004No Hit
TGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTT1551610.13478542526186782No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG1508730.1310605207850799No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC1413430.12278199007990527No Hit
TGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCG1390990.12083266973337728No Hit
GCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGG1359400.11808850619742277No Hit
TTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCG1290960.11214325287672862No Hit
AACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCAT1239660.10768691892945202No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1216980.10571675023697187No Hit
GTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGG1198780.10413575066893221No Hit
CGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGA1190760.10343906844169715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)