FastQCFastQC Report
Tue 14 Feb 2023
EGAF00002168875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115117046
Sequences flagged as poor quality0
Sequence length20-95
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGG2619710.22756925155984284No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC2436810.21168107458212573No Hit
ATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGC2031570.17647864244188477No Hit
CCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGG1976370.17168352287288538No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC1974610.17153063500256946No Hit
TTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGG1831690.15911544498805155No Hit
TCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCC1569530.13634210175962994No Hit
GTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTC1512390.13137845806085052No Hit
CGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGA1505600.13078862360662033No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC1448720.12584756561595578No Hit
GCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTG1427640.12401638589649008No Hit
GGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAG1420010.12335358223142731No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG1407600.12227554900948379No Hit
TTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGT1359660.1181110919055376No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1266630.11002975180582726No Hit
GGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTCTCAGGAC1212400.10531889430171791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)