FastQCFastQC Report
Fri 27 Jan 2023
EGAF00002168930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154155818
Sequences flagged as poor quality0
Sequence length20-125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT7389130.47932864914641105No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA6150650.3989891578402834No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT5877290.3812564505350035No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT5457050.3539957213940508No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAG5149420.33403993873264No Hit
CTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACC5007860.3248570222630196No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT4760360.30880183841001707No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCT4732850.3070172803987197No Hit
CGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCAC4304270.2792155402139931No Hit
CCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCA4283580.2778733917133118No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA4042370.2622262365731795No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG4041520.2621710975579267No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA4007870.25998824124821546No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGC3846840.24954231698215892No Hit
CGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGA3829520.24841877845959728No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT3769710.24453893786869596No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT3495320.22673941505081566No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA3350680.21735670073769128No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT3178170.2061660754185742No Hit
CCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAA3149940.2043348114178863No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA3146370.2041032275538248No Hit
CCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAA3016230.19566111997148236No Hit
CCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGC2998950.19454017622610909No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC2929700.19004796821875383No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG2704180.17541861443075732No Hit
CTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAG2655090.17223417412633754No Hit
CTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC2504050.16243629546307492No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC2327430.15097905678785342No Hit
CTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGT2286580.14832914058423666No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACC2220190.14402245914584944No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG2204070.1429767639389387No Hit
CCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCA2177090.14122658672538715No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG2061200.13370886851639943No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA2060990.13369524593616053No Hit
CGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCC2048310.1328727015674491No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC1992110.1292270396177976No Hit
CCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTA1953080.1266951857762514No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC1952590.12666339975569396No Hit
GTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGT1947260.12631764569534443No Hit
CCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGT1927700.1250487996502344No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT1920450.12457849628484342No Hit
TGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTC1891520.12270182368335912No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC1869610.12128053447843273No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC1867370.12113522695588433No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA1852350.12016088812165364No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAAC1848060.11988259826820159No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1842810.11954203376222881No Hit
CTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGAC1838390.11925531088291458No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGA1836470.11913076157787311No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC1807000.1172190594843459No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCG1806900.11721257254137499No Hit
CAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC1760930.11423052485764759No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC1753280.11373427372037298No Hit
CCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTT1734930.11254391968521096No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTA1717150.11139054122498315No Hit
CAGAAAACTACGATAGCCCTTATGAAACTTAAGGGTCGAAGGTGGATTTA1716510.11134902478996933No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG1709600.11090077703067944No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGA1699530.1102475418735088No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA1675680.10870040597494672No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGT1662630.10785385991724296No Hit
CCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCT1648520.10693855226404754No Hit
GTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTT1579940.10248980677459738No Hit
CTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC1547120.10036079209154466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)