FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002168948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138053291
Sequences flagged as poor quality0
Sequence length20-95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC5745300.41616537775981016No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC4460030.3230658224583723No Hit
TAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCT4393190.3182242138653544No Hit
TGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTT3147680.228004705805963No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGC3088390.22370998747143234No Hit
ATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGA2713560.19655887812192757No Hit
TAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC2502690.18128434185607353No Hit
AGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTT2461950.17833330753411739No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG2227480.1613492864867669No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA2089380.15134590308317966No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG2010510.14563289186637354No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG1766670.1279701474121323No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT1655840.1199420881607234No Hit
GAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAG1634930.1184274556699992No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT1593650.11543730601829695No Hit
CCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC1566400.11346343058203517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)