FastQCFastQC Report
Thu 26 Jan 2023
EGAF00002168983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002168983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151328303
Sequences flagged as poor quality0
Sequence length20-95
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT4536360.2997694357280938No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTT4131350.2730057707711161No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT3189850.21079004632728882No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC3183770.21038827085769934No Hit
GCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTT2920970.19302205483662896No Hit
CTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGG2815770.18607028190886407No Hit
GTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGA2520930.16658681489344396No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC2246010.14841969119286297No Hit
TGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGG2071740.13690366963277187No Hit
CTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGG1959340.12947610996470368No Hit
GTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGA1775680.11733958319746704No Hit
GCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTCCCAT1638010.10824214423391769No Hit
GCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGC1572860.10393693504908992No Hit
ATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCT1570270.10376578398556416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)