FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002169002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002169002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39346168
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT3582120.9104114027063576No Hit
GTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATT2951300.7500857516798078No Hit
CTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGG2059170.5233470258145596No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAG2041800.5189323646460311No Hit
CTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCC2021210.5136993264502911No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAA1932360.4911177118950948No Hit
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAA1839940.4676287662879902No Hit
GGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGA1812710.4607081431665721No Hit
GTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGT1783890.45338341461867393No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT1676980.42621177238911806No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT1564580.39764482274360236No Hit
CTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACC1420430.36100847228629734No Hit
CCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTT1232950.3133596135715173No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA1226760.3117863981061637No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT1184200.3009695887030219No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGAACAGCAGTTGAACATGGGTC1152420.2928925632605442No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA1092120.2775670555770514No Hit
CAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATA1086480.27613362500765004No Hit
GTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGAC1080490.27461124041355184No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC1068550.27157663739960647No Hit
CCCCGATCAACCTCACCACCTCTTGCTCAGCCTATATACCGCCATCTTCA1066840.27114203345037313No Hit
GTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA1062780.27011016676388916No Hit
GTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA1061120.26968827053246963No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTT954340.24254966836922978No Hit
CTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAG951720.24188378395578447No Hit
CGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCAC923820.2347928774156609No Hit
GCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCG921970.2343226918565488No Hit
CTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTA887000.22543491401754803No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGA878170.22319073105162365No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAGAAGACGGTCGAACTTGACTATCT876540.2227764594508924No Hit
GTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAA876370.22273325321032533No Hit
GAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTA870830.2213252380765517No Hit
GGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGG863780.21953344986480006No Hit
TGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGG850940.21627010793020557No Hit
GCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGG848670.21569317754145714No Hit
CTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGT826700.2101094063340552No Hit
GTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAA821240.20872172354878374No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC811860.20633775568690704No Hit
GGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGC810680.2060378535465004No Hit
GTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCT810200.20591585945548752No Hit
GGCGGAGATGGGCGCCGCGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGG798220.20287109026729108No Hit
CGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGG782050.19876141432629474No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGGG770860.195917427079557No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA767670.19510667468303394No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTA766530.19481693871687836No Hit
CTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCC763900.19414851275987027No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCC763560.19406210027873616No Hit
GTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTCA760910.19338859123460259No Hit
GGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGC758240.19270999910334344No Hit
CTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG747760.19004646144956225No Hit
GGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC733180.18634089093504608No Hit
CCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTA712480.18107989576011568No Hit
GGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTA702950.1786578047447975No Hit
CTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTC699300.17773014134438708No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC698330.17748361161879855No Hit
GGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCA697870.17736670061491122No Hit
GTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGAT694050.17639583097393371No Hit
CCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCTT680270.17289358394443902No Hit
CTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGT677080.17208283154791593No Hit
GGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTT670040.17029358487972704No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA632700.16080346121635022No Hit
CTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCC632160.16066621786396074No Hit
GGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCC624880.1588159741502654No Hit
GTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTG618940.15730629727398104No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA617230.15687169332474765No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG596760.15166915365176095No Hit
GTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTT590250.15001460879239878No Hit
GTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTC588980.14969183275992723No Hit
GTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGT587660.1493563490096418No Hit
GTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTG586430.1490437391514213No Hit
CTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGA576730.14657844189553607No Hit
CCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAA574450.1459989699632249No Hit
ACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGAT573470.1457498986940736No Hit
GCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC569340.14470024120265026No Hit
GTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGC564670.14351334035883748No Hit
GTGCATTTATCAGATCAAAACCAACCCGGTCAGCCCCTCTCCGGCCCCGG560420.14243318434466096No Hit
GGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCT559810.14227815018733211No Hit
CCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTA557550.14170376134214646No Hit
GCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTG536370.13632077207620322No Hit
GTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC529810.1346535194990272No Hit
GTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTA525970.13367756677092418No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG522910.13289985444071706No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA514720.13081833026280984No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA512460.1302439414176242No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA511860.13009144880385812No Hit
CTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC502910.12781676731518046No Hit
CCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAG498410.12667307271193473No Hit
CTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAA497750.12650533083679202No Hit
CCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGA496960.12630454889533332No Hit
CCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTC488840.12424081552236549No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC486720.1237020082870586No Hit
CCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAA482770.12269809857976513No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAA475350.12081227325619105No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC472690.12013622266849468No Hit
GTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGG466800.11863925351002416No Hit
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTC452540.11501501238951656No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG451950.11486506131931323No Hit
GGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAA451820.11483202125299724No Hit
CCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAG431330.10962439849288501No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG428910.10900934495069506No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC426140.10830533738380825No Hit
CGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGCC419010.10649321682355446No Hit
CTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCG418240.1062975179692213No Hit
CTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC415210.10552743026970252No Hit
CCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA410990.10445489888621427No Hit
CTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCAC409140.10398471332710214No Hit
CTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGT406510.10331628737009409No Hit
GGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGACTC405800.10313583777713754No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGG404890.10290455731292562No Hit
GTTTTAAGCAGGAGGTGTCAGAAAAGTTACCACAGGGATAACTGGCTTGT404000.10267835993583924No Hit
GTCCTGAGAGATGGGCGAGCGCCGTTCCGAAGGGACGGGCGATGGCCTCCGTTGCCCTCGGCCGATCGAAAGGG398790.10135421573963697No Hit
CTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGAC396700.10082303313501838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)