FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002169032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002169032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151443822
Sequences flagged as poor quality0
Sequence length20-95
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGC8623070.5693906747810419No Hit
TAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCT5311330.3507128867891356No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG4461440.29459372730305233No Hit
ATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGTGA4061670.26819648014430064No Hit
GTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGG3700230.24433020450315893No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG3459570.22843916340146248No Hit
TAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCC3147410.20782689966712542No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG2805910.1852772838762614No Hit
CTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACC2597960.17154611959014082No Hit
GTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGC2501990.165209116288679No Hit
GCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGG2419450.15975891046912433No Hit
AGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTT2412750.1593165021944573No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2315500.15289497910320832No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG2124020.14025134679973938No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA2069510.1366519923143514No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCA2057500.1358589589742393No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1993230.13161514109172442No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGCTTCCATGT1935350.1277932618472875No Hit
GGCCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCT1845230.12184254039758716No Hit
GAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAA1830380.12086197877388487No Hit
CCAAGCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTC1578830.10425185914814009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)