FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3815
Sequences flagged as poor quality0
Sequence length251-490
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA135835.59633027522936No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA133835.072083879423325No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA41810.956749672346003No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1473.853211009174312No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCATGTA1323.4600262123197902No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA661.7300131061598951No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA270.7077326343381389No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA170.4456094364351245No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGTA170.4456094364351245No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA110.28833551769331583No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA100.2621231979030144No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA90.235910878112713No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA80.20969855832241152No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGCGCACGTA80.20969855832241152No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA70.1834862385321101No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA60.15727391874180865No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGAGCATGTA60.15727391874180865No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA60.15727391874180865No Hit
TACGGAGGGGGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA50.1310615989515072No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA50.1310615989515072No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA40.10484927916120576No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40.10484927916120576No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA40.10484927916120576No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA40.10484927916120576No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA40.10484927916120576No Hit
TACGTAGGGGGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40.10484927916120576No Hit
TACGGGGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA40.10484927916120576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)