FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10772
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4334.019680653546231No Hit
CGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3783.5090976606015594No Hit
TGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2902.6921648718900855No Hit
GAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2612.422948384701077No Hit
TGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2532.3486817675454885No Hit
GCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2402.227998514667657No Hit
CTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2392.2187151875232085No Hit
TTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2342.1722985518009654No Hit
GGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2322.1537318975120683No Hit
TTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2162.005198663200891No Hit
CTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2131.9773486817675454No Hit
AGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2101.9494987003342No Hit
AGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2021.8752320831786111No Hit
GGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1991.8473821017452656No Hit
GTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1931.791682138878574No Hit
CCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1881.745265503156331No Hit
TGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1711.5874489417007056No Hit
GGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1691.5688822874118085No Hit
GTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1651.5317489788340142No Hit
CTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1621.5038989974006682No Hit
TACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1561.4481990345339768No Hit
AGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1561.4481990345339768No Hit
TCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1551.4389157073895285No Hit
GTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1401.2996658002227999No Hit
ACGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1351.253249164500557No Hit
ATCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1301.2068325287783142No Hit
GCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1291.1975492016338656No Hit
CGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1291.1975492016338656No Hit
AACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1261.1696992202005199No Hit
ATGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1231.1418492387671741No Hit
CCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1231.1418492387671741No Hit
TTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1221.1325659116227256No Hit
TCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1181.0954326030449313No Hit
CGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1181.0954326030449313No Hit
TTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1141.058299294467137No Hit
GGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1131.0490159673226884No Hit
CAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1131.0490159673226884No Hit
ATGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1030.9561826958782027No Hit
TAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1000.928332714444857No Hit
ACCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG970.9004827330115114No Hit
TTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG970.9004827330115114No Hit
GTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA960.8911994058670627No Hit
CACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG950.8819160787226141No Hit
ACGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG930.8633494244337171No Hit
CGGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA890.8262161158559227No Hit
TCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA870.8076494615670257No Hit
AGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG860.7983661344225771No Hit
GTGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG820.7612328258447828No Hit
CGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG810.7519494987003341No Hit
GAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG810.7519494987003341No Hit
ATGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA770.7148161901225399No Hit
TTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA750.6962495358336428No Hit
AAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG750.6962495358336428No Hit
GCGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA740.6869662086891942No Hit
CAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG720.6683995544002971No Hit
GCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG720.6683995544002971No Hit
CCGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG710.6591162272558485No Hit
GACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG700.6498329001113999No Hit
ACGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA690.6405495729669514No Hit
TCGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA680.6312662458225028No Hit
GCGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA660.6126995915336056No Hit
TGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG650.6034162643891571No Hit
GCGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG630.5848496101002599No Hit
ATGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG620.5755662829558114No Hit
TGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA620.5755662829558114No Hit
CAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA560.5198663200891199No Hit
TCGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA540.5012996658002228No Hit
CAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA530.4920163386557742No Hit
ATGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA510.47344968436687707No Hit
CTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA490.45488303007798No Hit
CTGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA460.42703304864463426No Hit
CCGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA450.4177497215001857No Hit
CAGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA440.40846639435573706No Hit
AGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA440.40846639435573706No Hit
TAGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.37133308577794283No Hit
CGGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.37133308577794283No Hit
GTGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG400.37133308577794283No Hit
TAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA390.3620497586334942No Hit
GGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA380.3527664314890457No Hit
AGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG380.3527664314890457No Hit
TAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.3434831043445971No Hit
GAGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.3434831043445971No Hit
GAGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG360.33419977720014854No Hit
ACGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA330.3063497957668028No Hit
AGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA330.3063497957668028No Hit
CCGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA320.29706646862235425No Hit
GGGGCTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG320.29706646862235425No Hit
ACGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.2877831414779057No Hit
GAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.2877831414779057No Hit
TGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG300.2784998143334571No Hit
TAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG300.2784998143334571No Hit
AAGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA290.26921648718900854No Hit
GCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA280.25993316004455996No Hit
TGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.2506498329001114No Hit
GACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.2506498329001114No Hit
CTGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.2506498329001114No Hit
GGGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG240.22279985146676567No Hit
GTCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA220.20423319717786853No Hit
CCGCTAGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA220.20423319717786853No Hit
AAGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA210.19494987003342No Hit
AAGCTAGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG160.14853323431117713No Hit
AAGGCTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA150.13924990716672855No Hit
TACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA150.13924990716672855No Hit
TCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA140.12996658002227998No Hit
ACCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA140.12996658002227998No Hit
ATCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA130.12068325287783142No Hit
TTCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA110.10211659858893427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)