FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58559
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA17733.027715637220581No Hit
CGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA17342.9611161392783347No Hit
AGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA16522.821086425656176No Hit
TGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG15262.605918816919688No Hit
TTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG14752.5188271657644425No Hit
GCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG11932.0372615652589694No Hit
TTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA10541.7998941238750663No Hit
TCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA10301.7589098174490683No Hit
ATACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG9271.5830188357041617No Hit
TAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG8901.5198346966307485No Hit
TAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA8851.5112962994586656No Hit
TGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG7831.3371129971481754No Hit
AGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG7691.3132054850663433No Hit
GGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG7651.306374767328677No Hit
GTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG7611.2995440495910109No Hit
TCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG7311.2483136665585137No Hit
GGGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6631.1321914650181868No Hit
CGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG6581.1236530678461039No Hit
AAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA6391.0912071585921892No Hit
AGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG6351.0843764408545227No Hit
AAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG6291.0741303642480233No Hit
GCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA5921.0109462251746102No Hit
GTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA5680.9699619187486125No Hit
GAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5580.9528851244044468No Hit
AGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5540.9460544066667804No Hit
GTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA5510.9409313683635309No Hit
TTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5260.8982393825031165No Hit
CTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5150.8794549087245341No Hit
CTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4900.8367629228641199No Hit
GGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4740.8094400519134548No Hit
TTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4720.8060246930446217No Hit
ACGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4640.792363257569289No Hit
ATGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4600.7855325398316229No Hit
CGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4570.7804095015283732No Hit
TGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4440.7582096688809578No Hit
CTGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4390.7496712717088748No Hit
GGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4270.729179118495876No Hit
GCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4250.7257637596270428No Hit
CAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4250.7257637596270428No Hit
TGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4240.7240560801926262No Hit
TTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4190.7155176830205434No Hit
GGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4030.6881948120698782No Hit
AGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3950.6745333765945456No Hit
ATCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3920.669410338291296No Hit
TACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3830.6540412233815468No Hit
ATACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3830.6540412233815468No Hit
ACACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3820.6523335439471302No Hit
TGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3790.6472105056438806No Hit
CGCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3770.6437951467750473No Hit
GTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3710.633549070168548No Hit
CTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3680.6284260318652982No Hit
TACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3660.6250106729964652No Hit
GGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3650.6233029935620484No Hit
GCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3590.6130569169555491No Hit
CCGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3590.6130569169555491No Hit
GCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3590.6130569169555491No Hit
TCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3550.6062261992178828No Hit
CTCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3530.6028108403490497No Hit
TGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3470.5925647637425502No Hit
CAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3450.5891494048737171No Hit
ATCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3430.5857340460048839No Hit
TCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3400.5806110077016343No Hit
CGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3160.5396267012756365No Hit
CGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3130.5345036629723868No Hit
ATCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3110.5310883041035537No Hit
GCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3090.5276729452347205No Hit
AACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3070.5242575863658874No Hit
GAGAATGGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3060.5225499069314709No Hit
CTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3060.5225499069314709No Hit
TATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3060.5225499069314709No Hit
TCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2960.5054731125873051No Hit
TTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2920.49864239484963885No Hit
ACGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2900.49522703598080564No Hit
GTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2820.4815656005054731No Hit
ACCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2810.4798579210710565No Hit
CCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2800.47815024163664No Hit
TCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2750.4696118444645571No Hit
ATTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2730.4661964855957239No Hit
TTCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2660.454242729554808No Hit
CCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2650.4525350501203914No Hit
CACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2590.44228897351389196No Hit
AACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2510.42862753803855935No Hit
CCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2460.42008914086647653No Hit
CTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2450.41838146143205995No Hit
GACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2420.4132584231288102No Hit
CGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2410.4115507436943937No Hit
TTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2400.40984306425997713No Hit
CGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2370.4047200259567274No Hit
CACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2360.4030123465223108No Hit
ATTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2240.382520193309312No Hit
GCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2210.37739715500606225No Hit
GTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2210.37739715500606225No Hit
GAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2120.3620280400963131No Hit
CATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2050.35007428405539714No Hit
CCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2010.34324356631773084No Hit
GGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2010.34324356631773084No Hit
ACACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1920.3278744514079817No Hit
GGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1860.31762837480148226No Hit
TTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1830.3125053364982326No Hit
ACCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1820.31079765706381596No Hit
GTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1800.3073822981949828No Hit
TAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1800.3073822981949828No Hit
AGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1780.30396693932614965No Hit
CGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1780.30396693932614965No Hit
AATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1730.29542854215406683No Hit
GACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1730.29542854215406683No Hit
GGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1710.2920131832852337No Hit
TCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1680.286890144981984No Hit
GGCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1680.286890144981984No Hit
ACTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1670.2851824655475674No Hit
TGCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1660.28347478611315086No Hit
CCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1630.27835174780990113No Hit
CTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1590.2715210300722348No Hit
GTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1580.26981335063781825No Hit
AGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1540.26298263290015195No Hit
TGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1530.2612749534657354No Hit
AGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1390.23736744138390342No Hit
CCCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1370.2339520825150703No Hit
TGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1300.22199832647415424No Hit
AGCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1290.2202906470397377No Hit
GTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1220.20833689099882172No Hit
GTCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1220.20833689099882172No Hit
ATGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1180.2015061732611554No Hit
GATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1170.19979849382673887No Hit
TTGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1170.19979849382673887No Hit
AGGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1150.19638313495790571No Hit
CAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1130.19296777608907256No Hit
GCCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1090.1861370583514063No Hit
CAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1050.17930634061373998No Hit
GGCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1020.17418330231049026No Hit
TATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1010.1724756228760737No Hit
GGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA970.16564490513840743No Hit
AAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG970.16564490513840743No Hit
AGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA940.1605218668351577No Hit
TGCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG920.15710650796632455No Hit
TAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG920.15710650796632455No Hit
TGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA890.15198346966307483No Hit
ATGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA820.14002971362215885No Hit
CCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA810.13832203418774228No Hit
TCCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG810.13832203418774228No Hit
CACAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG780.13319899588449255No Hit
TCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA750.12807595758124285No Hit
CGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG750.12807595758124285No Hit
GCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA740.12636827814682627No Hit
TGGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG730.12466059871240971No Hit
TTCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA700.11953756040916No Hit
CTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA680.11612220154032685No Hit
AGCAGGAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG680.11612220154032685No Hit
GACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA680.11612220154032685No Hit
ACTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA670.11441452210591027No Hit
GCGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA630.107583804368244No Hit
TTGACGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG630.107583804368244No Hit
CGCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA620.10587612493382743No Hit
GTCAGGAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.10416844549941086No Hit
GAGACGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.10416844549941086No Hit
CGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.10416844549941086No Hit
AAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA600.10246076606499428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)