FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11779
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA3923.327956532812633No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA3613.064776296799389No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA3322.8185754308515154No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2882.4450292894133625No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2622.224297478563545No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2492.1139315731386366No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2402.0375244078444688No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2372.012055352746413No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA2031.7234060616351132No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1901.6130401562102044No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1881.5960607861448342No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1871.5875711011121487No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1821.5451226759487224No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1761.4941845657526105No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1761.4941845657526105No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1631.3838186603277018No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1631.3838186603277018No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1611.3668392902623312No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1601.358349605229646No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1491.2649630698701078No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1471.2479836998047373No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1461.239494014772052No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1461.239494014772052No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1401.1885559045759402No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1311.1121487392817726No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1301.1036590542490874No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA1241.0527209440529757No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG1231.0442312590202902No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1211.0272518889549198No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT1191.0102725188895492No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1110.9423550386280668No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT1090.9253756685626964No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG1090.9253756685626964No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1060.8999066134646405No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1040.8829272433992699No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1010.857458188301214No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG1000.8489685032685287No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT990.8404788182358434No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG950.8065200781051022No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG940.7980303930724171No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG930.7895407080397318No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT920.7810510230070464No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT900.7640716529416758No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT900.7640716529416758No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT890.7555819679089906No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG890.7555819679089906No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG880.7470922828763052No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG850.7216232277782495No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG830.7046438577128789No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT800.679174802614823No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG800.679174802614823No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG790.6706851175821377No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG780.6621954325494523No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT750.6367263774513966No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG740.6282366924187113No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG720.6112573223533406No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT710.6027676373206554No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG680.5772985822225996No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT680.5772985822225996No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT670.5688088971899142No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG660.5603192121572289No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG640.5433398420918584No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT630.5348501570591732No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT630.5348501570591732No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG620.5263604720264878No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG620.5263604720264878No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT600.5093811019611172No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG560.47542236183037606No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT560.47542236183037606No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT550.46693267679769085No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT550.46693267679769085No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT550.46693267679769085No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT540.4584429917650055No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT520.44146362169963493No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG520.44146362169963493No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT510.43297393666694967No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT470.39901519653620854No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT470.39901519653620854No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG450.3820358264708379No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT440.3735461414381526No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG430.36505645640546736No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT420.35656677137278203No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG370.31411834620935564No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT360.3056286611766703No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG320.2716699210459292No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT310.2631802360132439No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT310.2631802360132439No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG300.2546905509805586No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG290.24620086594787333No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG290.24620086594787333No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT250.21224212581713217No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG230.1952627557517616No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG230.1952627557517616No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG230.1952627557517616No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT220.1867730707190763No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT220.1867730707190763No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC210.17828338568639102No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG210.17828338568639102No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT210.17828338568639102No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT200.16979370065370575No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT200.16979370065370575No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT200.16979370065370575No Hit
AGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG200.16979370065370575No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT190.16130401562102045No Hit
CCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT190.16130401562102045No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG190.16130401562102045No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG190.16130401562102045No Hit
CGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG180.15281433058833516No Hit
CATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT170.1443246455556499No Hit
TTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG170.1443246455556499No Hit
AATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG170.1443246455556499No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG160.1358349605229646No Hit
CTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC160.1358349605229646No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACATCGCTGAAGACAG150.1273452754902793No Hit
ACTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT150.1273452754902793No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC150.1273452754902793No Hit
TATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC140.11885559045759402No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC140.11885559045759402No Hit
TCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG140.11885559045759402No Hit
TGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC130.11036590542490873No Hit
GATCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG130.11036590542490873No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACTGGATACAGTGTCT130.11036590542490873No Hit
ATTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG120.10187622039222344No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG120.10187622039222344No Hit
GGTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG120.10187622039222344No Hit
GTTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC120.10187622039222344No Hit
GCTCACGGACGAGGCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT120.10187622039222344No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)