FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11779
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA7035.968248577977757No Hit
TTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG5474.6438577128788525No Hit
TGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4293.6420748790219886No Hit
GTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA4233.591136768825877No Hit
CTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3693.132693777060871No Hit
ATTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3532.9968588165379066No Hit
TAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA3452.928941336276424No Hit
TCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3242.7506579505900333No Hit
TATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3092.6233126750997537No Hit
TCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2952.50445708464216No Hit
GTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2842.4110705492826217No Hit
TGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2772.3516427540538247No Hit
CCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2311.9611172425503014No Hit
TTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2301.952627557517616No Hit
GGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2291.9441378724849308No Hit
CATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2261.918668817386875No Hit
GAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2261.918668817386875No Hit
ACTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2161.833771967060022No Hit
AGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2151.8252822820273369No Hit
AATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2101.7828338568639104No Hit
CTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2091.7743441718312252No Hit
ATTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2061.748875116733169No Hit
CAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2051.7403854317004839No Hit
GGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2001.6979370065370574No Hit
TGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1981.6809576364716867No Hit
ATGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1861.5790814160794635No Hit
ACGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1791.5196536208506666No Hit
CTGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1761.4941845657526105No Hit
CGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1731.4687155106545546No Hit
GCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1721.4602258256218694No Hit
GGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1621.3753289752950166No Hit
CGGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1581.3413702351642753No Hit
GCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1561.3243908650989047No Hit
AGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1541.3074114950335343No Hit
GTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1511.2819424399354784No Hit
CCGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1411.1970455896086256No Hit
AGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1381.1715765345105698No Hit
TTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1371.1630868494778843No Hit
TATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1261.0697003141183463No Hit
CGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1241.0527209440529757No Hit
AGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1110.9423550386280668No Hit
TGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1090.9253756685626964No Hit
GGGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1080.916885983530011No Hit
GCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1020.8659478733338993No Hit
GTGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1000.8489685032685287No Hit
ATGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG940.7980303930724171No Hit
GATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG930.7895407080397318No Hit
TAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG850.7216232277782495No Hit
TCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG770.6537057475167671No Hit
AAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG730.619747007386026No Hit
CAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG710.6027676373206554No Hit
TCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA710.6027676373206554No Hit
GATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA710.6027676373206554No Hit
CCGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG700.5942779522879701No Hit
GCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA630.5348501570591732No Hit
ACTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA590.500891416928432No Hit
GAGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG400.3395874013074115No Hit
CATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.3395874013074115No Hit
AATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA390.3310977162747262No Hit
AAGTTCAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.31411834620935564No Hit
ACGTTCAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG350.29713897614398505No Hit
CTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA350.29713897614398505No Hit
CGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA260.22073181084981747No Hit
CCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA260.22073181084981747No Hit
TTTTATAGAGATCTCTGCTTCTGATGGCTCACACACAGCGACCTCGGGTG130.11036590542490873No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)