FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44842
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT13853.088622273761206No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT9272.067258373845948No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT8911.9869764952499889No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT8711.9423754515855671No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA7741.7260603898131215No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7181.6011774675527408No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7051.5721867891708665No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT7021.5654966326212032No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT5921.3201908924668837No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT5711.273359796619241No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT5401.2042281789393872No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5331.1886178136568397No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4681.0436644217474689No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4641.0347442130145845No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4621.0302841086481422No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4601.0258240042817002No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4541.0124436911823738No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA4491.0012934302662682No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT4400.9812229606172784No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG4320.9633825431515096No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT4260.9500022300521832No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA4170.9299317604031934No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT4090.9120913429374248No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3960.8831006645555506No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3890.867490299273003No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3840.8563400383568975No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG3830.8541099861736765No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3820.8518799339904553No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3700.8251193077918023No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3640.8117389946924758No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3630.8095089425092548No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3570.7961286294099281No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3460.7715980553944962No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA3420.7626778466616119No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA3380.7537576379287275No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3370.7515275857455065No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3340.7448374291958432No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA3280.7314571160965166No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT3240.7225369073636323No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3220.7180768029971901No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGAAGATGGAAGGTT3170.7069265420810847No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT3030.6757058115159894No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2940.6556353418669997No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2940.6556353418669997No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA2770.6177244547522412No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2720.6065741938361358No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT2680.5976539851032514No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2670.5954239329200304No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2660.5931938807368092No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2640.5887337763703671No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2610.5820436198207039No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA2600.5798135676374827No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2580.5753534632710405No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA2550.5686633067213773No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2520.561973150171714No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG2510.559743097988493No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2500.5575130458052718No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA2370.5285223674233978No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2360.5262923152401766No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2300.5129120021408501No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2270.5062218455911869No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT2260.5039917934079657No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2250.5017617412247446No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2240.4995316890415236No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2220.4950715846750814No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2220.4950715846750814No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2190.4883814281254182No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG2160.4816912715757548No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2140.47723116720931275No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT2110.4705410106596494No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2100.46831095847642834No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA2090.4660809062932073No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT2010.4482404888274385No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1970.4393202800945542No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1960.43709022791133306No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1960.43709022791133306No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1960.43709022791133306No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1940.432630123544891No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1940.432630123544891No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1910.4259399669952276No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1890.42147986262878556No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1870.41701975826234333No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT1800.4014093929797957No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1790.3991793407965747No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1790.3991793407965747No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT1770.39471923643013246No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT1760.3924891842469113No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCACCTAAATCT1720.383568975514027No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1710.38133892333080593No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1640.3657285580482583No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1590.3545782971321529No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1560.3478881405824896No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1560.3478881405824896No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1530.34119798403282636No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1510.3367378796663842No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1500.3345078274831631No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1470.32781767093349984No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1460.32558761875027875No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1450.32335756656705766No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1410.3144373578341733No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCCACCAGTTCCTT1410.3144373578341733No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1410.3144373578341733No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1380.30774720128451005No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1380.30774720128451005No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT1350.3010570447348468No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1340.2988269925516257No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1340.2988269925516257No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1330.2965969403684046No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1320.29436688818518353No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1300.28990678381874135No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1240.27652647071941483No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1220.27206636635297266No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1200.2676062619865305No Hit
GCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1200.2676062619865305No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1200.2676062619865305No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1180.2631461576200883No Hit
AGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1160.25868605325364613No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG1130.25199589670398287No Hit
CAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1120.2497658445207618No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA1090.24307568797109852No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC1080.2408456357878774No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCCACATTAACAAA1080.2408456357878774No Hit
ACATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1060.23638553142143526No Hit
GAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1040.23192542705499308No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG1000.22300521832210873No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG970.2163150617724455No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGAAG950.2118549574060033No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT920.20516480085634006No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT910.20293474867311895No Hit
CGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA910.20293474867311895No Hit
CCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA900.20070469648989786No Hit
GGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA860.1917844877570135No Hit
GTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC780.1739440702912448No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT750.16725391374158155No Hit
TGATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT680.15164354845903394No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA670.14941349627581285No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT640.1427233397261496No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG630.1404932875429285No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC610.13603318317648633No Hit
TAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC610.13603318317648633No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC560.1248829222603809No Hit
AAATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT550.1226528700771598No Hit
CTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT530.11819276571071763No Hit
TTATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGCTGAGAGACTC470.1048124526113911No Hit
ATATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCAGAAGACAGAAAG450.10035234824494893No Hit
TCATGTCGATAAGCCCAGGGTTTTCCCAGTCACGACCCCTGATGGCTACA450.10035234824494893No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)