FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7759
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA5026.469905915710787No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3174.085578038407011No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA3124.021136744425828No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2753.5442711689650728No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG2543.273617734244104No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT2062.6549813120247454No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA2052.642093053228509No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA1722.2167805129527003No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1652.1265627013790436No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG1551.9976801134166773No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1461.8816857842505479No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG1291.6625853847145249No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG1281.6496971259182887No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1281.6496971259182887No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1251.6110323495295786No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT1221.5723675731408686No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG1141.4692615027709757No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA1121.4434849851785023No Hit
CATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1091.4048202087897925No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG1061.3661554324010827No Hit
CTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1041.3403789148086094No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA1041.3403789148086094No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG971.250161103234953No Hit
TGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA961.2372728444387164No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG951.2243845856424798No Hit
ACTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA941.211496326846243No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA881.1341667740688233No Hit
AATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA871.1212785152725866No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT871.1212785152725866No Hit
CCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA871.1212785152725866No Hit
TCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA871.1212785152725866No Hit
ATTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA811.0439489624951668No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG760.9795076685139837No Hit
TATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA680.8764015981440908No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG640.8248485629591443No Hit
TGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG620.799072045366671No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA590.7604072689779611No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG590.7604072689779611No Hit
TTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA580.7475190101817244No Hit
CATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG570.7346307513854878No Hit
CCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG510.6573011986080681No Hit
CTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG510.6573011986080681No Hit
TGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT500.6444129398118315No Hit
CCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT490.6315246810155948No Hit
CATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT470.6057481634231215No Hit
ACTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG470.6057481634231215No Hit
CATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG450.5799716458306483No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA450.5799716458306483No Hit
TTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG430.5541951282381751No Hit
CTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT410.5284186106457017No Hit
CCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA390.5026420930532285No Hit
CCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG380.48975383425699187No Hit
CATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA370.47686557546075525No Hit
AGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT370.47686557546075525No Hit
TCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG360.46397731666451864No Hit
AATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT350.45108905786828196No Hit
TCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT340.4382007990720454No Hit
AATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG340.4382007990720454No Hit
TGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG320.41242428147957216No Hit
ACTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG320.41242428147957216No Hit
TATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG320.41242428147957216No Hit
CTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA320.41242428147957216No Hit
ATTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTGTGAACTTCCAG320.41242428147957216No Hit
AATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG310.3995360226833355No Hit
TTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT300.3866477638870988No Hit
ACTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT290.3737595050908622No Hit
ATTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA290.3737595050908622No Hit
ATTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT290.3737595050908622No Hit
CTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG280.3608712462946256No Hit
TATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTCTGAGATGAAT270.34798298749838896No Hit
ACTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA260.33509472870215234No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG250.3222064699059157No Hit
TGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA250.3222064699059157No Hit
TCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG250.3222064699059157No Hit
AATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA230.29642995231344244No Hit
ATTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG220.2835416935172058No Hit
TTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA200.2577651759247326No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC200.2577651759247326No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC180.23198865833225932No Hit
TTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG170.2191003995360227No Hit
TATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCGATTCTCAGGGCG160.20621214073978608No Hit
TCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA150.1933238819435494No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC130.16754736435107617No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG130.16754736435107617No Hit
TATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGGACAAGTTTCTCA130.16754736435107617No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG130.16754736435107617No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG120.15465910555483955No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGTTCCCTAACT120.15465910555483955No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCTCTGAGCTGAATG110.1417708467586029No Hit
GTTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC110.1417708467586029No Hit
CGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCAGACCCCAGGACC110.1417708467586029No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCAGAATGCCCCAA100.1288825879623663No Hit
GATATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG90.11599432916612966No Hit
GGTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACGCTGAATGCCCCAA90.11599432916612966No Hit
GCTATGAGACTCAGCCAGGGTTTTCCCAGTCACGACCCTTCAATCTGAGG80.10310607036989304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)