FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7759
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG4285.516174764789277No Hit
TGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG3374.343343214331743No Hit
TTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2803.6087124629462557No Hit
AGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2673.4411650985951803No Hit
CTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2573.3122825106328135No Hit
GGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2513.2349529578553935No Hit
TGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2493.20917644026292No Hit
CGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2383.0674055935043176No Hit
TTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2162.7838638999871117No Hit
TCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2072.667869570820982No Hit
TAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA2042.6292047944322725No Hit
GCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1822.3456631009150666No Hit
TACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1772.281221806933883No Hit
ACGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1742.242557030545173No Hit
GGGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1702.191003995360227No Hit
GTCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1672.152339218971517No Hit
GGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1652.1265627013790436No Hit
ATCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1632.1007861837865702No Hit
GCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1592.049233148601624No Hit
AAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1531.9719035958242042No Hit
CTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1461.8816857842505479No Hit
AACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1381.778579713880655No Hit
TCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1311.6883619023069982No Hit
CACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1091.4048202087897925No Hit
TGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1081.3919319499935559No Hit
CGCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1071.3790436911973192No Hit
CCCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1061.3661554324010827No Hit
TCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1041.3403789148086094No Hit
CCGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1041.3403789148086094No Hit
TTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1031.3274906560123727No Hit
ACCCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1021.3146023972161363No Hit
TCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1011.3017141384198996No Hit
TGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG931.1986080680500064No Hit
GTGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG931.1986080680500064No Hit
GACCAACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG851.0955019976801135No Hit
CGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA821.0568372212914035No Hit
CCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA811.0439489624951668No Hit
ACGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA791.0181724449026937No Hit
ATGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG760.9795076685139837No Hit
GCGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA760.9795076685139837No Hit
CTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA710.9150663745328006No Hit
AGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG680.8764015981440908No Hit
CAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG650.8377368217553808No Hit
CGGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG630.8119603041629077No Hit
TTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG610.7861837865704343No Hit
AAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG570.7346307513854878No Hit
CAGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA500.6444129398118315No Hit
ATGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA490.6315246810155948No Hit
GTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG460.5928599046268849No Hit
CCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG460.5928599046268849No Hit
GGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA460.5928599046268849No Hit
TAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG450.5799716458306483No Hit
CTGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG420.5413068694419384No Hit
AGGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.5155303518494652No Hit
ATGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG360.46397731666451864No Hit
ACGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG360.46397731666451864No Hit
TAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG330.4253125402758087No Hit
AAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG310.3995360226833355No Hit
GTGAGACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA230.29642995231344244No Hit
GCGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG220.2835416935172058No Hit
GAGAATGGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG220.2835416935172058No Hit
GAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG170.2191003995360227No Hit
GGCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA150.1933238819435494No Hit
CAGAGACGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG140.1804356231473128No Hit
GCCCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA90.11599432916612966No Hit
GACCAACGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA80.10310607036989304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)