FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG24210.163796724065518No Hit
CTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1486.21587568248635No Hit
ATTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1415.921881562368752No Hit
TGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1385.795884082318354No Hit
GGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1305.459890802183956No Hit
TCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1184.955900881982361No Hit
TATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1164.871902561948762No Hit
ATTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1004.199916001679966No Hit
CCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG953.989920201595968No Hit
TTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA923.863922721545569No Hit
AATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG913.821923561528769No Hit
CATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG853.5699286014279714No Hit
CGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG833.485930281394372No Hit
GTTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG783.275934481310374No Hit
ACTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG733.0659386812263754No Hit
GGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG692.897942041159177No Hit
GTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA632.645947081058379No Hit
GCTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG632.645947081058379No Hit
AGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA572.393952120957581No Hit
AGTTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG532.2259554808903825No Hit
TGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA492.0579588408231837No Hit
TATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA361.5119697606047877No Hit
GATTATAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG341.4279714405711887No Hit
GCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA261.0919781604367913No Hit
ACTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA261.0919781604367913No Hit
TCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA241.007979840403192No Hit
CGTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA220.9239815203695927No Hit
GATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA210.881982360352793No Hit
CTTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA140.5879882402351954No Hit
AATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA110.46199076018479635No Hit
CATTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA70.2939941201175977No Hit
ATTTATAGAGATCTCTGCTTCTGATGGCTCACACACAGCGACCTCGGGTG30.125997480050399No Hit
CCTTATAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA30.125997480050399No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)