FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499
Sequences flagged as poor quality0
Sequence length251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA1041.3868515802106949No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA971.2935058007734364No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA951.266835578077077No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA831.10681424189892No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT791.0534737965062009No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA771.0268035738098413No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT761.0134684624616614No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA751.0001333511134818No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT740.9867982397653021No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA730.9734631284171223No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA730.9734631284171223No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA730.9734631284171223No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA730.9734631284171223No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA720.9601280170689425No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA710.9467929057207627No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT710.9467929057207627No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT710.9467929057207627No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA690.9201226830244034No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA690.9201226830244034No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT680.9067875716762234No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA680.9067875716762234No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT680.9067875716762234No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA660.8801173489798639No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT650.8667822376316843No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT650.8667822376316843No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT650.8667822376316843No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT640.8534471262835045No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA630.8401120149353246No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA630.8401120149353246No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT630.8401120149353246No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT620.826776903587145No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA620.826776903587145No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA620.826776903587145No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA620.826776903587145No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT620.826776903587145No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA610.8134417922389652No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA610.8134417922389652No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA600.8001066808907855No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA600.8001066808907855No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA590.7867715695426056No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT590.7867715695426056No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT580.7734364581944259No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT570.7601013468462462No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT560.7467662354980664No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA550.7334311241498866No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA550.7334311241498866No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT550.7334311241498866No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA550.7334311241498866No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA540.720096012801707No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT540.720096012801707No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT530.7067609014535271No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT520.6934257901053474No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA520.6934257901053474No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT520.6934257901053474No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCTGAATGCCCCAA510.6800906787571676No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA500.6667555674089879No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA500.6667555674089879No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA500.6667555674089879No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT500.6667555674089879No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT490.6534204560608081No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA490.6534204560608081No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT490.6534204560608081No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA480.6400853447126283No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA480.6400853447126283No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA460.6134151220162688No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA460.6134151220162688No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA450.600080010668089No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT440.5867448993199094No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT440.5867448993199094No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA420.5600746766235498No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT420.5600746766235498No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA420.5600746766235498No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA420.5600746766235498No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT410.54673956527537No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT410.54673956527537No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT410.54673956527537No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT410.54673956527537No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCATCAACGGTTTT400.5334044539271903No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA390.5200693425790106No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA380.5067342312308307No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT370.49339911988265106No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA370.49339911988265106No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT370.49339911988265106No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT370.49339911988265106No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT360.48006400853447123No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT350.4667288971862915No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT350.4667288971862915No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA330.44005867448993197No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT330.44005867448993197No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT330.44005867448993197No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT320.42672356314175225No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA320.42672356314175225No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCAGAGAGGCCTGA310.4133884517935725No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGGGTACAGTGTCT310.4133884517935725No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT310.4133884517935725No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT310.4133884517935725No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT300.40005334044539276No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT300.40005334044539276No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCCTCGCTGGATAA300.40005334044539276No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA290.38671822909721293No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA290.38671822909721293No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT290.38671822909721293No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA290.38671822909721293No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT290.38671822909721293No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT280.3733831177490332No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA260.3467128950526737No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT250.33337778370449395No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA250.33337778370449395No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT240.3200426723563142No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA240.3200426723563142No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA230.3067075610081344No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT230.3067075610081344No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA230.3067075610081344No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT220.2933724496599547No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT220.2933724496599547No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT220.2933724496599547No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT220.2933724496599547No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA210.2800373383117749No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT210.2800373383117749No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT210.2800373383117749No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT190.25336711561541536No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCGGTTCTTTGCAGT190.25336711561541536No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT180.24003200426723562No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT180.24003200426723562No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT180.24003200426723562No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA180.24003200426723562No Hit
GATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT170.22669689291905584No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT170.22669689291905584No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT170.22669689291905584No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA170.22669689291905584No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA150.20002667022269638No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT150.20002667022269638No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT150.20002667022269638No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTGCAGAGAGGCCT140.1866915588745166No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC140.1866915588745166No Hit
ATTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGCTGACCTTAACAA140.1866915588745166No Hit
TTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC140.1866915588745166No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT140.1866915588745166No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT130.17335644752633686No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT130.17335644752633686No Hit
AATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT130.17335644752633686No Hit
ACTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC120.1600213361781571No Hit
TCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC120.1600213361781571No Hit
TGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC120.1600213361781571No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT100.13335111348179757No Hit
GTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT100.13335111348179757No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTGGATACAGTGTCT100.13335111348179757No Hit
CGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC90.12001600213361781No Hit
GCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACTCTCCACTCTGACG90.12001600213361781No Hit
CATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC90.12001600213361781No Hit
CTTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT90.12001600213361781No Hit
CCTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC80.10668089078543806No Hit
TATATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCTCAGCAGAGATGC80.10668089078543806No Hit
AGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACGGTACAGCGTCTCT80.10668089078543806No Hit
GGTATGAGAGGAATCCAGGGTTTTCCCAGTCACGACCCAGTGATCGCTTC80.10668089078543806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)