FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002195139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002195139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499
Sequences flagged as poor quality0
Sequence length251
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG2022.693692492332311No Hit
TACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1872.4936658221096146No Hit
TTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1852.4669955994132553No Hit
TGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1842.453660488065075No Hit
GTACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1702.2669689291905586No Hit
GCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1622.1602880384051204No Hit
TCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1612.1469529270569407No Hit
TGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1602.133617815708761No Hit
GGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1552.0669422589678623No Hit
CTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1542.0536071476196827No Hit
AGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1532.0402720362715026No Hit
ATCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1481.9735964795306042No Hit
GCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1461.9469262568342447No Hit
GTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1461.9469262568342447No Hit
CTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1441.920256034137885No Hit
AACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1441.920256034137885No Hit
GTCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1381.8402453660488067No Hit
TTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1331.7735698093079078No Hit
TGACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1321.7602346979597279No Hit
TGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1291.7202293639151889No Hit
CGCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1241.65355380717429No Hit
CACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1241.65355380717429No Hit
ACCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1231.6402186958261102No Hit
TTACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA1191.5868782504333914No Hit
CCCCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1141.5202026936924924No Hit
TAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1071.426856914255234No Hit
GACCTGTGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1061.4135218029070542No Hit
TCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1051.4001866915588745No Hit
ATACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG1031.373516468862515No Hit
ATACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG991.320176023469796No Hit
CAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG941.2535004667288971No Hit
CCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG931.2401653553807175No Hit
GGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA931.2401653553807175No Hit
ATACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA931.2401653553807175No Hit
GCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG881.1734897986398187No Hit
GCACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA881.1734897986398187No Hit
ACACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG831.10681424189892No Hit
TCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG821.09347913055074No Hit
CGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG791.0534737965062009No Hit
GTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG791.0534737965062009No Hit
TAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG720.9601280170689425No Hit
GGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG710.9467929057207627No Hit
GTACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG710.9467929057207627No Hit
GGACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA700.933457794372583No Hit
CTACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG690.9201226830244034No Hit
GCACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG680.9067875716762234No Hit
AAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG670.8934524603280437No Hit
AGACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG640.8534471262835045No Hit
TGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG630.8401120149353246No Hit
GCCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.8134417922389652No Hit
ACACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.8134417922389652No Hit
TTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA610.8134417922389652No Hit
CGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG570.7601013468462462No Hit
CGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA560.7467662354980664No Hit
ACACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG540.720096012801707No Hit
GAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA540.720096012801707No Hit
TCACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA520.6934257901053474No Hit
AAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG490.6534204560608081No Hit
GAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG490.6534204560608081No Hit
CAACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG470.6267502333644486No Hit
CTCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA420.5600746766235498No Hit
GAACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG420.5600746766235498No Hit
CGCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.5334044539271903No Hit
GGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG400.5334044539271903No Hit
GACCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA400.5334044539271903No Hit
TAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA380.5067342312308307No Hit
GGCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.49339911988265106No Hit
CCACTTAGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG370.49339911988265106No Hit
CTACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA370.49339911988265106No Hit
TCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA340.4533937858381117No Hit
CCCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA330.44005867448993197No Hit
ATACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA330.44005867448993197No Hit
TTCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA310.4133884517935725No Hit
CTACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA300.40005334044539276No Hit
GTCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA290.38671822909721293No Hit
AGACCGCGAGATCTCTGCTTCTGATGGCTCAAACACAGCGACCTCGGGTG290.38671822909721293No Hit
GAACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.3600480064008535No Hit
CGACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.3600480064008535No Hit
AGACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA270.3600480064008535No Hit
TAACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA260.3467128950526737No Hit
CACCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA250.33337778370449395No Hit
AAACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA240.3200426723563142No Hit
CCACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA240.3200426723563142No Hit
TGCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA240.3200426723563142No Hit
CAACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA230.3067075610081344No Hit
TCCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA200.26670222696359513No Hit
ACACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA190.25336711561541536No Hit
CAACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA180.24003200426723562No Hit
AGACCGCGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA180.24003200426723562No Hit
CCACTTAGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA160.21336178157087612No Hit
AGCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA120.1600213361781571No Hit
ACCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA100.13335111348179757No Hit
TACCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA90.12001600213361781No Hit
ATCCTGTGTCACTGGATTTAGAGTCTCTCAGCTGGTACACGGCAGGGTCA90.12001600213361781No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)