FastQCFastQC Report
Thu 9 Feb 2023
EGAF00002207412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences231655409
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC2428830010.484667767891404No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG26601211.1483094702960293No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA25967711.120962817665095Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC22704440.9800953967796193TruSeq Adapter, Index 9 (100% over 50bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA21054490.9088710723780251Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC19684780.8497440264820235No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT19617220.8468276257689281Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG18127010.7824988882517309Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT17821550.7693129237487393No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC16281990.7028538668829443No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC15278300.6595270132457818No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC13275550.5730731717988937No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG13145800.5674721801984774Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA12965220.5596769812527882No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC12542550.5414313464185073No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA12442990.5371335836151359Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC12049760.5201588019039088No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT11519660.4972756755271792Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT11061440.4774954337457322Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC9443150.4076377944622049No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC9342720.403302475877004No Hit
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG8362090.3609710662961468Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG8238690.3556441887355197Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG8190840.3535786207349037No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA7530920.32509148102818525No Hit
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG7169930.3095084216229115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT7007150.30248160533993834Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA6900780.29788987141673Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGCAGCGAGGGAGCNGCTCTGCTACGTAGATCGGAAGAGCACACGTC6781370.29273523244173416No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT6740780.2909830609653496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC6604160.2850855081911772No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT6521770.2815289324843695No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT6506280.28086026689754523Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG6426700.2774249920492899Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT6322840.2729416087150376No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC6286900.2713901664173963No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC6224960.26871636742140564No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG6133610.26477301032932066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG6102650.26343654250697857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACTC5996800.2588672557177372Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT5975540.25794951327901006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG5458140.23561461498185868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT5385490.23247849136127877Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT5341400.23057523340627029No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT5329440.23005894932502957No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT4811210.20768822194866168Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4510260.19469694316526837No Hit
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC4468750.1929050575287884No Hit
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA4414830.19057746240667317Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG4390240.18951597197542666Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT4354830.18798740848740553Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT4330880.1869535452979645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT4316120.1863163920338247No Hit
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC4294930.18540167132467No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT4269070.18428535808546564Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG4213570.18188955821014308Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT4138240.17863774551450254Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC4122340.17795138122589663No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT4055170.17505181586327648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG4033930.17413493677585573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG4005150.17289257424591367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC3893600.1680772323343419No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA3861560.16669414354145298Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT3745930.161702677963371No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC3665530.15823200571155235No Hit
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG3662770.15811286323126605Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG3658080.15791040734990996Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA3602550.15551331244762776Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC3549950.15324269851173647No Hit
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC3518250.1518742866910567No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG3513530.151670535782741No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT3498430.151018705546392Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3432470.1481713729378104No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA3401970.14685476219551602Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG3325430.14355071674583691Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT3296540.14230360578370954No Hit
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG3240480.13988363207180712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG3231040.1394761302551757No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC3220810.13903452606194056No Hit
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA3189590.13768683467261497Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA3176420.13711831783733572Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG3130270.1351261346977657No Hit
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC3119940.13468021374799843No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT3115430.13448552802840016Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACAC3098760.13376592471449697No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC3028170.13071872627847858No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2990560.12909519414675097No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC2989960.12906929360755828No Hit
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT2983460.12878870443297094Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC2982770.12875891881289936No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG2944210.1270943774941167No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA2869550.12387148706724133Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAGGGCAGGGACTTAATCAACGCAAGCTTAGATCGGAAGAGCACACGTCT2847690.12292784408932149Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAGCACACGTC2822570.12184347484845476No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG2822530.12184174814584192No Hit
ACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGATCGGAAGAGCACACGT2803440.1210176793238616No Hit
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC2801250.12092314235580832Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGCTACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCT2793280.12057909686019894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTTGGAGACCTGCTGCGGATATGGGTAGATCGGAAGAGCACACGTCTGA2765740.11939026211125509Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC2745000.1184949668064949No Hit
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAG2727760.11775075798035867Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT2641660.11403403060620959Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC2610640.1126949727299482No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG2582230.11146858219917499Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACACG2557980.11042176874013765No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTAGATCGGAAGAGCACACGTC2520090.10878614969012013No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTCAGATCGGAAGAGCACACG2501580.10798711805602605No Hit
ACGACGGTCTAAACCCAGCTCACGTTCCCTAGATCGGAAGAGCACACGTCT2498310.10784596011742596Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACCAGATCGGAAGAGCACACGT2473980.10679569325316293No Hit
ACGGATCCGGCTTGCCGACTTCCCTTACCTACAGATCGGAAGAGCACACGT2427940.10480825854577822No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTG2416220.10430233468021462Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACACCTGCGTTACCGTTTGACAGGTGTAGATCGGAAGAGCACACGTCT2415900.10428852105931186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2411330.10409124528579429Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2391020.1032145120341222Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCAGATCGGAAGAGCACACGTC2378840.10268873108851087No Hit
ACGGAATCGAGAAAGAGCTATCAATCTGTCAGATCGGAAGAGCACACGTCT2376000.10256613520299887Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2371410.10236799607817489No Hit
CGAAGAAGCGGGGCGCGCCGACCGGGGTCGCAGATCGGAAGAGCACACGTC2351970.10152881860833217No Hit
AAGACCTCTAATCATTCGCTTTACCGGATAGATCGGAAGAGCACACGTCTG2329840.10057352038777563Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)