FastQCFastQC Report
Wed 22 Feb 2023
EGAF00002207415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203871586
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC90306584.429581471936948No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA38303821.8788209162212532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG38223541.8748831433527968No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC20402711.0007628036993836No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATAGATCGGAAGAGCACACGTCT20401561.0007063956425983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG17306900.8489118243284771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC15238480.7474548218798868No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT14775970.7247684824505166Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT14713960.7217268619276842Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCT14315480.7021812249991521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT14033480.68834898846571No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC11762220.5769425858098735No Hit
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG10639990.5218966609697145No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC10176350.4991548944932424No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA9594120.47059623110010046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC9300860.45621168611500384No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGATCGGAAGAGCACACGTC9266890.4545454411680498No Hit
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC9197280.45113103696559265No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC8989360.4409324602988079No Hit
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA8594640.4215712531907217Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT8265960.40544934005663746Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC8116360.3981113876261305No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT7910380.3880079688986184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTGAAC7711880.37827144779263155Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA7362880.3611528288203929Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT6182220.3032408841906984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG5494150.26949071755394105No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG5433020.2664922614571704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT5401270.26493490858505414Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC5349710.26240586562170565No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC5200300.25507723278318933No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC5096380.2499799064691634No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCAGATCGGAAGAGCA4844230.23761182688793128No Hit
AGTGAAAAAACGATCAGAGTAGTGGTAGATCGGAAGAGCACACGTCTGAAC4781720.2345456811230183Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA4741420.23256894661132424Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG4718540.2314466715337173Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT4666510.2288945748428131No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA4411230.21637296724615662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC4394490.2155518621412991Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACAAAGGGCAGGGACTTAATCAACGCAGATCGGAAGAGCACACGTCTGAAC4345930.213169970630434Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA4333020.21253672887991365Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG4318540.2118264778692603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA4175380.20480441055675114No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT3988760.19565060920259872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA3986770.19555299873911808No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT3939960.19325694557553497No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC3916150.19208905354765818No Hit
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT3906670.19162405495781054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTGCCAGACTAGAGTCAAGCTCAACAGATCGGAAGAGCACACGTCTGA3902060.19139793222582768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC3888380.19072692160250326No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGTCTGA3856650.1891705497400702Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACCTGTCTCACGACGGTCTAAACCCAGCTCAGATCGGAAGAGCACACGTC3847980.1887452820423931No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG3795440.18616816960456667Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCAGATCGGAAGAGCACACGTCTGA3780840.185452032535814Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT3612190.1771796683820373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC3582150.1757061918378366No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG3494840.17142359406572721Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT3459280.16967935884895702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGTGGCCCACTAGGCACTCGCATTCCAGATCGGAAGAGCACACGTCTG3451540.16929970810154976Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC3427330.16811219588000853No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT3400620.1668020574480644Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG3387290.16614821449419637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG3349450.16429214417353874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC3301840.16195685062262674No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC3279400.1608561577580507No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC3258700.15984081273591505No Hit
ACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGGGGCAGATCGGAAGAGC3231600.15851154461514808No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAACT3226030.1582383334183705Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCAGATCGGAAGAGCACACGTC3105860.15234393673672603No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT3042650.1492434556329002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT3041400.1491821425276988Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACT3009250.14760516946191804Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACA2928940.1436659250789367No Hit
ACTGAGCAGGATTACCATGGCAACAACAGATCGGAAGAGCACACGTCTGAA2927050.14357321966387215Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGTTCCGCCTGGGCGGGATTCTGACTTAGATCGGAAGAGCACACGTCTG2918920.14317443922764206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG2897330.142115439274603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC2811920.1379260374223998No Hit
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT2781630.13644029825715878No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT2780830.1364010578698299Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGATCGGAAGAGCACACG2779900.13635544091956003No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCAC2778470.13628529872720957No Hit
CTTGCGCCGGTCCAAGAATTTCACCTCTAGATCGGAAGAGCACACGTCTGA2749990.13488834093830024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG2742400.1345160477635172Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2736200.1342119347617181Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTCAGATCGGAAGAGCACAC2728300.13382443593684507No Hit
TAGCAATAAACGAAAGTTTAACTAAGCTAGATCGGAAGAGCACACGTCTGA2727890.133804325238339Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACA2719700.13340260177305924No Hit
AAGGGCATCACAGACCTGTTATTGCTCAGATCGGAAGAGCACACGTCTGAA2672800.13110213406590165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2660110.13047968342189675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA2646990.12983614106970257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTCTGAA2631180.1290606529151149Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTCACGTTCCCTATTAGTGGGTGAACAGATCGGAAGAGCACACGTCTGA2603340.1276950874360687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT2534080.12429785090306797Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT2512320.1232305123677215Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2507160.12297741186945Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2496210.1224403090678855Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG2404180.11792619301053556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT2379090.1166955163629325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGAAGGATCAAAAAGCGACGTCGCTAGATCGGAAGAGCACACGTCTGAA2377440.11661458306406662Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGGGAATTCCTCGTTCATGGGGAATAATTGCAGATCGGAAGAGCACACG2370330.1162658341216809No Hit
ATGCGCGTCACTAATTAGATGACGAGGCAGATCGGAAGAGCACACGTCTGA2364020.11595632556662408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGATCGGAAGAGCACACGTCTGA2342090.11488064844897022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAAC2297600.112698392408641Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCCAACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTG2280560.11186257215853512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT2274590.1115697407680931No Hit
ACCGTTTGACAGGTGTACCGCCCCAGTCAGATCGGAAGAGCACACGTCTGA2263850.11104293856820244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCTCTCAGGACCGACTGACCCATGTTCAGATCGGAAGAGCACACGTCTGA2260630.11088499600920357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2252770.11049945920369698No Hit
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC2245370.11013648562090451No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACGTC2190850.10746225322443904No Hit
ACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGTCTGAA2161500.1060226215143095Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGGTCTGTATTCGTACTGAAAATCAAGATCAGATCGGAAGAGCACACGTC2143630.1051460893623499No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGATCGGAAGAGCACACGTCTGAA2113130.10365004959543504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT2084130.10222758555476191Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGACGCCTCGCGGCGCCCATCTCCGCCACTAGATCGGAAGAGCACACG2066970.1013858792465567No Hit
ACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTGAACT2061860.10113523127249326Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)