FastQCFastQC Report
Tue 23 May 2023
EGAF00002207416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105271943
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC26834522.549066658720263No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC21955212.0855708913817614No Hit
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA8594600.81641886290633Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT7959350.7560751491021687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT7840300.7447663429181696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG6928610.6581630207015368No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC6454240.613101631457491No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAGATCGGAAGAGCACA6401340.6080765508431815No Hit
AGACGTGGCGACCCGCTGAATTTAAGCATAGATCGGAAGAGCACACGTCTG6283240.5968579871276812Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTGCGAGAATTAATGTGAATTGCAGGAGATCGGAAGAGCACACGTCTGA6079680.5775214009301605Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5807690.5516845072385527No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC5329140.5062260511331116No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC5259700.4996298016461993No Hit
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG4857380.4614125911972576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT4855190.4612045585593495No Hit
ATCTAAGGCTAAATACCGGCACGAGACCGATAGATCGGAAGAGCACACGTC4622080.439060956631151No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG4018770.38175128960999605No Hit
AGTACTTGGATGGGAGACCGCCTGGGAATAGATCGGAAGAGCACACGTCTG3996030.3795911698903477Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGCACACGTCTGA3839210.36469451314297485Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC3834970.3642917467572533No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGAGCACACGT3726620.35399935574476854No Hit
AGGATTCAACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTC3567950.33892696366400304No Hit
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC3545740.33681718974256986No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACGT3517340.334119414894812No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT3425680.32541244156574556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT3296790.3131689133922417No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG3254870.3091868457296357No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG3178160.30190000387852634Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC3095480.29404605935695516No Hit
TCTTCAGCAAACCCTGATGAAGGCTACAAAGTAGATCGGAAGAGCACACGT2896300.2751255384352505No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG2891920.27470947315943434Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT2825700.2684190981446975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT2712280.25764509732664476Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2686010.2551496555924687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT2677430.25433462361381515No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG2667800.25341984995945216Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT2623280.2491908029093754No Hit
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2568870.24402228426618858No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT2511030.2385279428156845Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC2497070.23720185348911058No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2470500.23467791413330333No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC2436820.23147858114483552No Hit
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC2422970.23016294094619305No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACG2422070.2300774480812993No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAGATCGGAAGAGCACACGTC2396850.22768174802283264No Hit
AAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGTC2381500.2262236197160339No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA2380360.22611532875383522No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA2358890.22407584896575908Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTG2358310.22402075356393866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGATTAAGAGGGACGGCCGGGGGCATTCGTAGATCGGAAGAGCACACGTC2354340.22364363503768522No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG2338210.22211141291464528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC2269730.21560635581695306No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCAGATCGGAAGAGCACA2269500.2155845076403691No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC2200670.20904620331744042No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG2198280.2088191722651115Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAAGATCGGAAGAGCACACG2198040.20879637416780653No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2139770.20326118612629768Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCTGCGAGAATTAATGTGAATTGCAGAGATCGGAAGAGCACACGTCTGAA2108690.20030883252530068Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAATTTAAGCATATTAGATCGGAAGAGCACACGT2063290.19599619245177224No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT2046180.19437087809807024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT1976130.18771668344717451No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT1975590.1876653877282383No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAGATCGGAAGAGCACACGTCT1939940.1842789203577253Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC1932760.18359687728001753No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA1926780.1830288246888347No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC1869130.17755253173203045No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC1791370.17016594820521172No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGT1769060.16804667507656812No Hit
ATAGGCGATAGAAATTGAAACCTGGCGCAGATCGGAAGAGCACACGTCTGA1730640.16439707966632666Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATAGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTC1725030.1638641741418224No Hit
ATCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA1721300.1635098537128739Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAGATCGGAAGAGCAC1703740.16184179292672501No Hit
AGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCGGAAGAGCACAC1693830.1609004214921729No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCAGATCGGAAGAGCACACGT1618520.15374656854200933No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT1603890.15235683452712562No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG1538710.1461652512673771Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCAGATCGGAAGAGCAC1537590.14605886014662045No Hit
ACGTGTTAGGACCCGAAAGATGGTGAACTAGATCGGAAGAGCACACGTCTG1491790.14170822324425036Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC1472190.13984637863100902Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA1467680.1394179643858193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGCAGATCGGAAGAGCACACGTC1447490.1375000744500365No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT1440370.13682373089665495No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG1428170.13566482761698434No Hit
AGCGCCGAATCCCCGCCCCGCGGCGGGGCGCGGGACAGATCGGAAGAGCAC1420960.1349799347771134No Hit
TTTATCCGGTAAAGCGAATGATTAGAGGTAGATCGGAAGAGCACACGTCTG1414080.13432638932103685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG1399520.13294330475120042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACG1392130.13224131333835076No Hit
ACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCAGATCGGAAGAGCACAC1377900.1308895761523087No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCAGATCGGAAGAGCACAC1355750.12878550175520176No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC1347260.12797901906303752No Hit
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC1318640.12526034595941676No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAGAAGATCGGAAGAGCACACGTC1269090.12055348878665609No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGATAAGATCGGAAGAGCACACGTCTG1202570.11423461615028802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1201990.1141795207484676No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT1196900.1136960111014575No Hit
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1160830.1102696470606608Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTGA1146320.10889131209442958Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCTAAATACCGGCACGAGACCGATAGTCAGATCGGAAGAGCACACGTC1128920.10723845003981734No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA1126280.1069876709694624Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCAACAAGTACCGTAAGGGAAAGTTGAAGATCGGAAGAGCACACGTCTG1121030.1064889625909156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTGAA1120370.10642626782332687Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCAGATCGGAAGAGCACACGTCT1113550.10577842189157657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT1105510.10501468563185919No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA1084250.10299515417892496Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1075710.10218392188315552No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCGGAAGAGCACACGT1064160.10108676345035258No Hit
AAAGGACCTGGCGGTGCTTCATATCCCTCTAGAGATCGGAAGAGCACACGT1063460.10102026899987968No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1060300.10072009405203056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAGCAATAAACGAAAGTTTAACTAAGCTAGATCGGAAGAGCACACGTCTG1056320.10034202560505603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTTAACACAAAGCACCCAACTTACACTTAGATCGGAAGAGCACACGTCTG1055060.10022233559420482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)