FastQCFastQC Report
Tue 23 May 2023
EGAF00002207417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99098335
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC78101867.881248458917095No Hit
AGGAGAGGAGCGAGCGACCAAAGGAACCAGATCGGAAGAGCACACGTCTGA10363131.0457420904195818Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG9214270.9298107783546514No Hit
ATGGCCTCAGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTG8499200.8576531583502387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCAGATCGGAAGAGCACACGTC7544350.7612993699641876No Hit
CGGGAGGGAGGAAGACGAACGGAAGGACAGATCGGAAGAGCACACGTCTGA7279170.73454009091071Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTAGATCGGAAGAGCACACGTC6188630.6244938424041131No Hit
ACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACTCCAG6097660.6153140716239077Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACA5974200.6028557392008655No Hit
ACCGGGTCAGTGAAAAAACGATCAGAGTAGATCGGAAGAGCACACGTCTGA5567180.5617834043326762Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCCAG5514840.5565017817907839Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATGATAGGAAGAGCCGACATCGAAGGATCAGATCGGAAGAGCACACGTCT5007940.5053505692098661Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAA4885330.4929780101754485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGAACTC4548950.45903394845130346Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACAC4523320.4564476285096011No Hit
ACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGCACACGTCTGAACTC4008680.40451537354285516Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGAGC3867320.3902507544652491No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTCTG3849110.38841318575130446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT3551250.35835617217988575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT3483250.35149430108992247No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATCGGAAGAGCACAC3391690.34225499348702476No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGTCT3338780.3369158523198195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCACA3314620.3344778698855031No Hit
ACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAACT2957250.2984157100116768Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTG2955050.2981937082999427Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGACTACCATCGAAAGTTGATAGGGCAGATCGGAAGAGCACACGTCT2827430.28531559082198504Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCAGATCGGAAGAGCACACG2571750.25951495552372295No Hit
ACTTCGGCCTTCAAAGTTCTCGTTTGAATAGATCGGAAGAGCACACGTCTG2557780.2581052446542114Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAGATCGGAAGAGCACACGTC2524910.2547883372611659No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTC2479730.2502292293810991No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGCAC2387630.24093543044895757No Hit
AAACGCTTCGGGCCCCGCGGGACAGATCGGAAGAGCACACGTCTGAACTCC2379210.2400857693522298Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTCTTTGGACACTAGGAAAAAACCTTGTAGATCGGAAGAGCACACGTCT2376860.23984863116015015Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGAGAGGAGCGAGCGACCAAAGGAACCATAGATCGGAAGAGCACACGTCT2343050.23643686849027282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTAGATCGGAAGAGCACACGT2340680.23619771210081378No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCAGATCGGAAGAGCACACGT2305720.23266990308162092No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGATCGGAAGAGCACACGTCTGAA2254430.22749423590214707Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTAGCGACGGGCGGTGTGTACAAAGGGCAGATCGGAAGAGCACACGTCTG2206800.22268789884310364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGACGAAGGGCACTCCGCACCGGACCCCGGTAGATCGGAAGAGCACACGT2102240.21213676294359537No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCAGATCGGAAGAGCACACGTC2093970.21130223832721307No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2067680.20864931787199048No Hit
AACCGAGGCTCCGCGGCGCTGCCGTAGATCGGAAGAGCACACGTCTGAACT2035340.2053858927094991Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGAAGGACCCCACACCCCCGCCGCAGATCGGAAGAGCACACGTCTGAACTC1995070.20132225228607523Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGAGCGGCGCCGGGGAGCGGGTCTTCCGTAGATCGGAAGAGCACACGTCT1977160.1995149565328217Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACACG1961400.1979246169978537No Hit
AGAGCACTGGGCAGAAATCACATCGCGTCAGATCGGAAGAGCACACGTCTG1935940.19535545173387628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGAACGGCCATGCACCACCACCCACGGAATAGATCGGAAGAGCACACGTC1917730.19351788301993167No Hit
CCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCAGATCGGAAGAGCACACGT1877310.1894391061161623No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACCAGATCGGAAGAGCACAC1859200.18761162838911472No Hit
AGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCTGAAC1855170.1872049616171654Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTGAA1841780.18585377847165646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGTAGCGCGCGTGCAGCCCCGGACATCTAGATCGGAAGAGCACACGTCT1839850.18565902242454427Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGTTTGACAGGTGTACCGCCCCAGATCGGAAGAGCACACGTCTGAACTC1835880.18525841024473316Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGAGAGGAGCGAGCGACCAAAGGAACAGATCGGAAGAGCACACGTCTGAA1834990.18516860046134984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGTGTTAGACTCCTTGGTCCGTGTTTCAGATCGGAAGAGCACACGTCTGA1832460.18491329849285562Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCCGGACATCTAAGGGCATCACAGACCTGTTAGATCGGAAGAGCACAC1783540.1799767877028408No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCT1763680.17797271770509565Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1709890.17254477585319672TruSeq Adapter, Index 1 (100% over 50bp)
AGAGGCGTTCAGTCATAATCCCACAGATGGTAGATCGGAAGAGCACACGTC1709680.172523584780713No Hit
CTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAACTC1703940.17194436213282493Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGCTTGGTGAATTCTGCTTCACAGATCGGAAGAGCACACGTCTGAACTC1673110.16883331087247833Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCACCACCCACGGAATCGAGAAAGAGCTAGATCGGAAGAGCACACGTCTG1663470.16786053973560708Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACGTC1645400.1660370984033183No Hit
ACGAATGCCCCCGGCCGTCCCTCTTAATCAGATCGGAAGAGCACACGTCTG1643600.1658554606391722Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGAGGGAGCTGCTCTGCTACGTACGAAACAGATCGGAAGAGCACACGTC1635720.1650602908716882No Hit
ACCGGGTCAGTGAAAAAACGATCAGATCGGAAGAGCACACGTCTGAACTCC1621730.16364856180479723Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTAGGGACAGTGGGAATCTCGTTCATCCAGATCGGAAGAGCACACGTCT1608490.16231251513963377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC1570450.15847390372401313No Hit
CCTGAGGGAAACTTCGGAGGGAACCAGATCGGAAGAGCACACGTCTGAACT1548740.15628315046867336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCACAC1544430.15584822893341246No Hit
AATCGGTAGTAGCGACGGGCGGTGTGTACAGATCGGAAGAGCACACGTCTG1542710.15567466395878396Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCACCCACGGAATCGAGAAAGAGCTATCAGATCGGAAGAGCACACGTCTG1535480.15494508560613052Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGGAGGAAGGACGCGCGAGGGCCGGGACCCCAGATCGGAAGAGCACACGT1516360.1530156889114232No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACAGATCGGAAGAGCACACGTCT1502010.15156763229170298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTACAGATCGGAAGAGCACACG1475940.1489369120076538No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCAGATCGGAAGAGCACACGTCT1455720.1468965144570794Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCAGATCGGAAGAGCAC1438760.14518508307934738No Hit
CTGACACCTCCTGCTTAAAACCCAAAAGGTCAGATCGGAAGAGCACACGTC1426670.1439650827634995No Hit
AATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCAGATCGGAAGAGCACAC1415690.14285709240220837No Hit
ACTGAGCAGGATTACCATGGCAACAACACAGATCGGAAGAGCACACGTCTG1409020.14218402357617813Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGTCTCTTCACCGTGCCAGACTAGAGTCAGATCGGAAGAGCACACGTCTG1407790.14205990443734498Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCTTGGTGAATTCTGCTTCACAATGATAGATCGGAAGAGCACACGTCT1407610.14204174066093037Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACGGGGAGGAGGCGGGAACCGAAGAAGCAGATCGGAAGAGCACACGTCT1399140.14118703407075406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACTTGAGGAGGGTGACGGGCGGTGTGTAGATCGGAAGAGCACACGTCTG1371360.1383837579107661Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGATAGGAAGAGCCGACATCGAAGGATAGATCGGAAGAGCACACGTCTG1360650.13730301321409688Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCCTGAGGGAAACTTCGGAGGGAACAGATCGGAAGAGCACACGTCTGAAC1351280.13635748774184753Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGTAGGCACACGCTGAGCCAGTCAGTGTAGATCGGAAGAGCACACGTCT1342070.13542810784863338Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGGCAGGATCAACCAGGTAGGTAGGCAGATCGGAAGAGCACACGTCTGA1335520.13476714820687957Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGACCGACTGACCCATGTTCAACTGCTGTTCAGATCGGAAGAGCACACGT1332890.13450175525148833No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTAGATCGGAAGAGCAC1304320.13161876029501404No Hit
AGGCGGCTCGGGCCTGCTTTGAACAGATCGGAAGAGCACACGTCTGAACTC1287020.1298730195618322Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGTCT1286430.12981348273913987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCGACTACCATCGAAAGTTGATAGATCGGAAGAGCACACGTCTGAACT1270730.1282291977963101Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGACGAAGGGCACTCCGCACCGGACCCCAGATCGGAAGAGCACACGTCTG1267160.12786894956408704Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGCTACGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCT1264390.12758942922703997Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGATAGAAATTGAAACCTGGCGCAATAGATCGGAAGAGCACACGTCTG1251380.12627659183173967Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGTCCACGGGCTGGGCCTCGATCAGATCGGAAGAGCACACGTCTGAACT1218340.12294252976096924Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTAGATCGGAAGAGCACACGTCTGAA1212370.12234009784321805Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCAGATCGGAAGAGCACACGT1209080.12200810437430658No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAGATCGGAAGAGCACACGTCT1193340.12041978303671802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACGTC1184860.11956406734785201No Hit
AGCTCTAGAATTACCACAGTTATCCAAGTAGATCGGAAGAGCACACGTCTG1180670.1191412549968675Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTCTGAAC1169360.11799996437881624Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTATCCTGAGGGAAACTTCGGAGGGAACAGATCGGAAGAGCACACGTCT1164110.11747018756672349Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACAC1160720.11712810311091504No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACACG1157730.1168263826026946No Hit
AGGATTACCATGGCAACAACACATCATCAGTAGATCGGAAGAGCACACGTC1132490.11427941750989055No Hit
AGAGTAGTGGTATTTCACCGGCGGCCCGCAGATCGGAAGAGCACACGTCTG1128140.11384045957987085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTCCGAAAACCAACAAAATAGATCGGAAGAGCACACGTCTGAACTCCAG1120800.11309978114163069Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATGGGCCCGACGCTCCAGCGCCATCCAGATCGGAAGAGCACACGTCTGAAC1100770.1110785564661606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGCCAGCGAGAGCTCACCGGACGCCGCAGATCGGAAGAGCACACGTCT1100720.1110735109727121Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACACCTTTTCTGGGGTCTGATGAGCGTCGGCAGATCGGAAGAGCACACGT1087670.10975663718265298No Hit
ATTCCACGCCCGGCTCCACGCCAGATCGGAAGAGCACACGTCTGAACTCCA1083930.10937923427270498Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAACCCTTGTGTCGAGGGCTGACTTTCAGATCGGAAGAGCACACGTCTGAA1082320.1092167693836632Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGCACTGGGCAGAAATCACATCGCGTCAACAGATCGGAAGAGCACACGT1075260.10850434570873467No Hit
ACGCGCGCGCGCGCGCGCCGCCCCCGCCGCTCCCGTAGATCGGAAGAGCAC1067770.10774853079014898No Hit
AGCGAGGGAGCTGCTCTGCTACGTACGAAACCAGATCGGAAGAGCACACGT1061070.10707243466804967No Hit
AGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTCTGAACTC1060510.10701592514142644Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCAGATCGGAAGAGCACACGTC1059210.10688474231176537No Hit
AATCATGGCCTCAGTTCCGAAAACCAACAGATCGGAAGAGCACACGTCTGA1054800.10643972978960747Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTATCCAAGTAGGAGAGGAGCGAGCGACCAGATCGGAAGAGCACACGTC1026320.10356581672134046No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTAGATCGGAAGAGCACACGTCT1025670.10350022530650994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTAGATCGGAAGAGCACAC1007990.10171613882311947No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC1003690.10128222638654827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)