FastQCFastQC Report
Tue 23 May 2023
EGAF00002207418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110980871
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT33922593.056615946003884Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA17734411.5979699780874852Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG17718901.5965724399477816Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT15809951.424565319909951Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC14308261.2892546139775747No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC8971040.8083411059190552No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCTG7124620.641968290193001Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG5361490.48310037141445755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTG5333910.48061525846197406Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC5217640.47013867822320476No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT4857170.43765830599761646Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAAC4826020.4348515159878318Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCT4599470.4144380881638602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTG4223470.3805583756862027Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTGA4120490.3712792991145294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTAGATCGGAAGAGCACACGTC3970390.3577544458089539No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3936850.3547323033714522TruSeq Adapter, Index 4 (100% over 50bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTGA3888630.3503874104574292Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG3714590.3347054286499518No Hit
AGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGAA3431810.3092253619094411Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGAA3232690.2912835311952093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT3036500.273605709942572No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT2868480.2584661639572103Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCT2808140.25302919094949256Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2804660.2527156233978376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTATAGGTAGAGGCGACAAACCTACAGATCGGAAGAGCACACGTCTGAAC2801040.2523894410596219Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGA2678290.24132897641432277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG2653730.23911598242908005Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTCT2639800.2378608111662775Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTG2504280.2256496977753941Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCTG2473170.2228465119903411Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC2458900.22156070481731938No Hit
ATCTTCAGCAAACCCTGATGAAGGCTACAGATCGGAAGAGCACACGTCTGA2409500.21710948727371224Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACGTC2344180.21122378828690216No Hit
AAAGCACCCAACTTACACTTAGGAGATTAGATCGGAAGAGCACACGTCTGA2308590.2080169293319026Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC2282420.20565886530121033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCAGATCGGAAGAGCACACGTC2269830.20452443556691854No Hit
TTTATAGGTAGAGGCGACAAACCTAAGATCGGAAGAGCACACGTCTGAACT2266610.20423429547601946Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGAA2230850.20101211856591034Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT2131370.19204841165825776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCTGA2073800.1868610312132079Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTATTACCCTCACTGTCAACCCAACACAGATCGGAAGAGCACACGTCTGA1979150.17833253444190397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1968750.17739543601167088No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGA1944270.17518965047589147Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC1941930.17497880332908902Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG1939310.17474272660916493No Hit
TAACCCAAGTCAATAGAAGCCGGCGTAGATCGGAAGAGCACACGTCTGAAC1867630.16828395589002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGCCTGGTGATAGCTGGTTGTCCAAGAAGATCGGAAGAGCACACGTCTGA1805930.16272443924142566Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1793870.16163776548482844No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAACAGATCGGAAGAGCACACGTCTG1724820.15541597254179054Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTTCAGCAAACCCTGATGAAGGCTACAAGATCGGAAGAGCACACGTCTGA1720700.155044737394429Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTTTGCAAGGAGAGCCAAAGCTAAGACAGATCGGAAGAGCACACGTCTG1712050.15426532379620628Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACCAGTGAAATTGACCTGCCCGTGAAGATCGGAAGAGCACACGTCTGAA1673220.1507665226379418Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT1657970.14939241195899428No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTCT1598470.14403112767064155Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTATTACCCTCACTGTCAACCCAACAGATCGGAAGAGCACACGTCTGAAC1581170.14247230047419612Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGCATGCTCATAAGGAAAGGTTAAAAAAGATCGGAAGAGCACACGTCTGA1580480.1424101275975749Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1578290.14221279629351619Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCTCTAGCATCACCAGTATTAGAGGCAGATCGGAAGAGCACACGTCTGA1563480.140878332086617Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA1544130.1391347883726737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA1527540.13763993616521536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTCT1504780.13558913229289757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGATCAAAAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGT1504020.13552065202299593No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGTC1435560.12935202139475008No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACACG1345800.12126414109689228No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1328640.1197179286870077Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTTTGCAAGGAGAGCCAAAGCTAAGACCCAGATCGGAAGAGCACACGT1322990.11920883194365992No Hit
TTAACCAGTGAAATTGACCTGCCCGTGAGATCGGAAGAGCACACGTCTGAA1264190.11391062158811135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTGAA1239870.1117192529512586Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTTGCAAGGAGAGCCAAAGCTAAGAAGATCGGAAGAGCACACGTCTGAA1221830.11009374759727737Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATGCTAAGACTTCACCAGTCAAAGCGAAAGATCGGAAGAGCACACGTCTG1210610.10908276256004514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC1203900.10847815386130823No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTGA1198780.10801681309565501Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC1178750.10621199756127343No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1172360.10563622266038983No Hit
ATGGAGCTTTAATTTATTAATGCAAACAGAGATCGGAAGAGCACACGTCTG1165340.1050036812199825Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGACAAGCATCAAGCACGCAGCAGATCGGAAGAGCACACGTCTGAACT1164420.10492078405115418Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATTAAAGTCCTACGTGATCTGAGTAGATCGGAAGAGCACACGTCTGAA1161290.10463875346590135Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTGGCTTTAACATATCTGAACACACAATAGATCGGAAGAGCACACGTCTG1154490.10402603526151817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGATTAAGCCATGCATGTCTAAGTACGAGATCGGAAGAGCACACGTCTGA1151110.10372147827169244Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGTCT1124070.10128502235308642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAACT1121310.10103633084660149Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCTGA1114380.1004118989118404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)