FastQCFastQC Report
Thu 16 Mar 2023
EGAF00002207420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002207420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181839357
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC167603289.21710694346549No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT152922058.409733323023135Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT119923896.595045867875567Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC74807344.113924577944916No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT50532272.7789512036164976Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG48902672.6893336407915256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGT40052212.202615025744949No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTC37693312.072890633901659No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGTC32813781.804547736054742No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTCT31534001.7341680327213214Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCTG30465761.6754216745278085Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTC20219551.1119457489062723No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACG19457681.0700477784905498No Hit
GGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCT18728871.0299678963339054Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTG13133870.7222787308910249Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACCTGCATTAAAAATTTCGGTTGGGAGATCGGAAGAGCACACGTCTGAA12106300.6657689622164689Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTG10902220.5995522740437319Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTCTG10575570.5815886161542025Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC10564720.580991935645703No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTCT9966960.548118964147019Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT9629770.5295756737635186Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT8964010.49296313778760226No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGTCT7630240.4196143302464494Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTC7491300.4119735201219393No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTC7030430.38662862187749597No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGAGATCGGAAGAGCACACGTCT6816290.3748522933899288Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCTGA6298830.3463953075900945Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTCT6240280.34317543258800676Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGT5758820.31669821621729555No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT5742300.3157897220237091No Hit
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG5681910.3124686588063551No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT5345820.29398586137763344No Hit
CTAAACCATTCGTAGACGACCTGCTTCAGATCGGAAGAGCACACGTCTGAA5050090.2777226054533398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4999000.2749129826718426No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGTCT4517390.24842751726184337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC4342400.23880418802844755No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACACGT3920930.21562603743698894No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGAGATCGGAAGAGCACACGTCTG3817350.20992980084063978Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCT3720430.2045998215886784Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTAGATCGGAAGAGCACACGTC3406700.1873466809498232No Hit
CAACTTCTTAGAGGGACAAGTGGCGTTAGATCGGAAGAGCACACGTCTGAA3347380.18408446087939037Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCCGGGTTAAGGCGCCCGATGCCGACGCTCAGATCGGAAGAGCACACGT3335980.1834575338935014No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCAGATCGGAAGAGCACACGTCTGA3281820.18047908077457622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATAGATCGGAAGAGCACACGT3097120.1703217637312697No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAAGATCGGAAGAGCACACGTCTG3005910.16530579790820532Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCAGATCGGAAGAGCACACGTCTGA2964160.16300981530637507Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTGAA2931100.16119172704729703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACAC2771390.15240869994937345No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC2711170.14909698564321255No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGT2633770.1448404813705979No Hit
GTGTGGGGCCCAAGTCCTTCTGATCGAGGCAGATCGGAAGAGCACACGTCT2432180.13375432250346111Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT2272240.12495864687862926No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGATCGGAAGAGCACACGTCTGAAC2255370.12403090492670407Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGCCGCGACCGGCTCCGGGACGGCTGGGAAGATCGGAAGAGCACACGTC2220530.12211492806807495No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAAGAGATCGGAAGAGCACACGT2155000.11851119777111839No Hit
CTAAACCATTCGTAGACGACCTGCTTAGATCGGAAGAGCACACGTCTGAAC2120610.11661996803035331Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGT2076630.11420134971110792No Hit
CAACTTCTTAGAGGGACAAGTGGCGTAGATCGGAAGAGCACACGTCTGAAC2044580.11243880498323583Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGTC2009510.11051017959769842No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCTGAA1937680.10655998965064532Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACGTC1931190.10620308121745063No Hit
CCGGGGAGGTAGTGACGAAAAATAACAAAGATCGGAAGAGCACACGTCTGA1856060.10207141240606125Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTGCGCCGCGACCGGCTCCGGGACGGCTGGAGATCGGAAGAGCACACGT1839010.10113377160699046No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)